Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis
The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had d...
Gespeichert in:
| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2016-01, Vol.2016 (2016), p.1-11 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 2016 |
| container_start_page | 1 |
| container_title | Evidence-based complementary and alternative medicine |
| container_volume | 2016 |
| creator | Cuesta-Rubio, Osmany Monteiro, Marta Chagas de Campos, Mônica Soares Torres, Yohandra Reyes Ferreira, Antonio Gilberto Sawaya, Alexandra Christine Helena Frankland Ferreira, Roseane Guimarães Cardoso, Magda Vieira de Oliveira, Fátima de Cassia Evangelista Lira, Tatiana Onofre de Mokochinski, João Benhur Machado, Christiane Schineider Pessoa, Claudia |
| description | The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing 1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated. |
| doi_str_mv | 10.1155/2016/6057650 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4972909</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A610316799</galeid><sourcerecordid>A610316799</sourcerecordid><originalsourceid>FETCH-LOGICAL-c569t-cd3a6e78b0072f33b4c69f8e6139fbb402f5753c5dcea57db830ca3779dbfcc73</originalsourceid><addsrcrecordid>eNqNkd1rFDEUxQdRbK2--SwDvgju2nxMvl6E7aJVKCh-gD6FTCbZTckkazLTpf3rm-muW_XJpxu4v3NuDqeqnkPwBkJCThGA9JQCwigBD6pjyBo4bxDnDw9v9uOoepLzJQBIMMYeV0eIEUQAwsfVuIz9RiU1uCtTfx3G7rqOtl6uTe-08vW0jdkNLoZaha4-c9HH1d1qoYvGDXf8T-N93M7q82RMmNVnKW7LmARfTBEldeO8U6H-nOImepefVo-s8tk828-T6vv7d9-WH-YXn84_LhcXc02oGOa6w4oaxlsAGLIYt42mwnJDIRa2bRuALGEEa9JpowjrWo6BVpgx0bVWa4ZPqrc7383Y9qZQYUjKy01yvUrXMion_94Et5areCUbwZAAohi82huk-Gs0eZC9y7qkVcHEMUvIIeQN5hwU9OU_6GUcUyjxCgWYgIDR5p5aKW-kCzaWu3oylQsKAYaUiensbEfpFHNOxh6-DIGcWpdT63LfesFf_BnzAP-uuQCvd8DahU5t3X_amcIYq-5pCBpEOL4FlOe-hg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1807910764</pqid></control><display><type>article</type><title>Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis</title><source>Wiley Online Library Open Access</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Cuesta-Rubio, Osmany ; Monteiro, Marta Chagas ; de Campos, Mônica Soares ; Torres, Yohandra Reyes ; Ferreira, Antonio Gilberto ; Sawaya, Alexandra Christine Helena Frankland ; Ferreira, Roseane Guimarães ; Cardoso, Magda Vieira ; de Oliveira, Fátima de Cassia Evangelista ; Lira, Tatiana Onofre de ; Mokochinski, João Benhur ; Machado, Christiane Schineider ; Pessoa, Claudia</creator><contributor>Prieto, Jose M.</contributor><creatorcontrib>Cuesta-Rubio, Osmany ; Monteiro, Marta Chagas ; de Campos, Mônica Soares ; Torres, Yohandra Reyes ; Ferreira, Antonio Gilberto ; Sawaya, Alexandra Christine Helena Frankland ; Ferreira, Roseane Guimarães ; Cardoso, Magda Vieira ; de Oliveira, Fátima de Cassia Evangelista ; Lira, Tatiana Onofre de ; Mokochinski, João Benhur ; Machado, Christiane Schineider ; Pessoa, Claudia ; Prieto, Jose M.</creatorcontrib><description>The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing 1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2016/6057650</identifier><identifier>PMID: 27525023</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Bacteria ; Biological activity ; Comparative analysis ; Cytotoxicity ; Ethanol ; Flavonoids ; Methicillin ; Multivariate analysis ; RNA polymerase ; Studies</subject><ispartof>Evidence-based complementary and alternative medicine, 2016-01, Vol.2016 (2016), p.1-11</ispartof><rights>Copyright © 2016 Christiane Schineider Machado et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 Christiane Schineider Machado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 Christiane Schineider Machado et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-cd3a6e78b0072f33b4c69f8e6139fbb402f5753c5dcea57db830ca3779dbfcc73</citedby><cites>FETCH-LOGICAL-c569t-cd3a6e78b0072f33b4c69f8e6139fbb402f5753c5dcea57db830ca3779dbfcc73</cites><orcidid>0000-0003-1566-0223</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972909/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972909/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27525023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Prieto, Jose M.</contributor><creatorcontrib>Cuesta-Rubio, Osmany</creatorcontrib><creatorcontrib>Monteiro, Marta Chagas</creatorcontrib><creatorcontrib>de Campos, Mônica Soares</creatorcontrib><creatorcontrib>Torres, Yohandra Reyes</creatorcontrib><creatorcontrib>Ferreira, Antonio Gilberto</creatorcontrib><creatorcontrib>Sawaya, Alexandra Christine Helena Frankland</creatorcontrib><creatorcontrib>Ferreira, Roseane Guimarães</creatorcontrib><creatorcontrib>Cardoso, Magda Vieira</creatorcontrib><creatorcontrib>de Oliveira, Fátima de Cassia Evangelista</creatorcontrib><creatorcontrib>Lira, Tatiana Onofre de</creatorcontrib><creatorcontrib>Mokochinski, João Benhur</creatorcontrib><creatorcontrib>Machado, Christiane Schineider</creatorcontrib><creatorcontrib>Pessoa, Claudia</creatorcontrib><title>Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing 1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated.</description><subject>Bacteria</subject><subject>Biological activity</subject><subject>Comparative analysis</subject><subject>Cytotoxicity</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Methicillin</subject><subject>Multivariate analysis</subject><subject>RNA polymerase</subject><subject>Studies</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkd1rFDEUxQdRbK2--SwDvgju2nxMvl6E7aJVKCh-gD6FTCbZTckkazLTpf3rm-muW_XJpxu4v3NuDqeqnkPwBkJCThGA9JQCwigBD6pjyBo4bxDnDw9v9uOoepLzJQBIMMYeV0eIEUQAwsfVuIz9RiU1uCtTfx3G7rqOtl6uTe-08vW0jdkNLoZaha4-c9HH1d1qoYvGDXf8T-N93M7q82RMmNVnKW7LmARfTBEldeO8U6H-nOImepefVo-s8tk828-T6vv7d9-WH-YXn84_LhcXc02oGOa6w4oaxlsAGLIYt42mwnJDIRa2bRuALGEEa9JpowjrWo6BVpgx0bVWa4ZPqrc7383Y9qZQYUjKy01yvUrXMion_94Et5areCUbwZAAohi82huk-Gs0eZC9y7qkVcHEMUvIIeQN5hwU9OU_6GUcUyjxCgWYgIDR5p5aKW-kCzaWu3oylQsKAYaUiensbEfpFHNOxh6-DIGcWpdT63LfesFf_BnzAP-uuQCvd8DahU5t3X_amcIYq-5pCBpEOL4FlOe-hg</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Cuesta-Rubio, Osmany</creator><creator>Monteiro, Marta Chagas</creator><creator>de Campos, Mônica Soares</creator><creator>Torres, Yohandra Reyes</creator><creator>Ferreira, Antonio Gilberto</creator><creator>Sawaya, Alexandra Christine Helena Frankland</creator><creator>Ferreira, Roseane Guimarães</creator><creator>Cardoso, Magda Vieira</creator><creator>de Oliveira, Fátima de Cassia Evangelista</creator><creator>Lira, Tatiana Onofre de</creator><creator>Mokochinski, João Benhur</creator><creator>Machado, Christiane Schineider</creator><creator>Pessoa, Claudia</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1566-0223</orcidid></search><sort><creationdate>20160101</creationdate><title>Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis</title><author>Cuesta-Rubio, Osmany ; Monteiro, Marta Chagas ; de Campos, Mônica Soares ; Torres, Yohandra Reyes ; Ferreira, Antonio Gilberto ; Sawaya, Alexandra Christine Helena Frankland ; Ferreira, Roseane Guimarães ; Cardoso, Magda Vieira ; de Oliveira, Fátima de Cassia Evangelista ; Lira, Tatiana Onofre de ; Mokochinski, João Benhur ; Machado, Christiane Schineider ; Pessoa, Claudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-cd3a6e78b0072f33b4c69f8e6139fbb402f5753c5dcea57db830ca3779dbfcc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacteria</topic><topic>Biological activity</topic><topic>Comparative analysis</topic><topic>Cytotoxicity</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Methicillin</topic><topic>Multivariate analysis</topic><topic>RNA polymerase</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cuesta-Rubio, Osmany</creatorcontrib><creatorcontrib>Monteiro, Marta Chagas</creatorcontrib><creatorcontrib>de Campos, Mônica Soares</creatorcontrib><creatorcontrib>Torres, Yohandra Reyes</creatorcontrib><creatorcontrib>Ferreira, Antonio Gilberto</creatorcontrib><creatorcontrib>Sawaya, Alexandra Christine Helena Frankland</creatorcontrib><creatorcontrib>Ferreira, Roseane Guimarães</creatorcontrib><creatorcontrib>Cardoso, Magda Vieira</creatorcontrib><creatorcontrib>de Oliveira, Fátima de Cassia Evangelista</creatorcontrib><creatorcontrib>Lira, Tatiana Onofre de</creatorcontrib><creatorcontrib>Mokochinski, João Benhur</creatorcontrib><creatorcontrib>Machado, Christiane Schineider</creatorcontrib><creatorcontrib>Pessoa, Claudia</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cuesta-Rubio, Osmany</au><au>Monteiro, Marta Chagas</au><au>de Campos, Mônica Soares</au><au>Torres, Yohandra Reyes</au><au>Ferreira, Antonio Gilberto</au><au>Sawaya, Alexandra Christine Helena Frankland</au><au>Ferreira, Roseane Guimarães</au><au>Cardoso, Magda Vieira</au><au>de Oliveira, Fátima de Cassia Evangelista</au><au>Lira, Tatiana Onofre de</au><au>Mokochinski, João Benhur</au><au>Machado, Christiane Schineider</au><au>Pessoa, Claudia</au><au>Prieto, Jose M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>2016</volume><issue>2016</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing 1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>27525023</pmid><doi>10.1155/2016/6057650</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1566-0223</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2016-01, Vol.2016 (2016), p.1-11 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4972909 |
| source | Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Bacteria Biological activity Comparative analysis Cytotoxicity Ethanol Flavonoids Methicillin Multivariate analysis RNA polymerase Studies |
| title | Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T07%3A49%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20Study%20of%20Chemical%20Composition%20and%20Biological%20Activity%20of%20Yellow,%20Green,%20Brown,%20and%20Red%20Brazilian%20Propolis&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Cuesta-Rubio,%20Osmany&rft.date=2016-01-01&rft.volume=2016&rft.issue=2016&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2016/6057650&rft_dat=%3Cgale_pubme%3EA610316799%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1807910764&rft_id=info:pmid/27525023&rft_galeid=A610316799&rfr_iscdi=true |