Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

The CRISPR/Cas9 system enables genome editing and somatic cell genetic screens in mammalian cells. We performed genome-scale loss-of-function screens in 33 cancer cell lines to identify genes essential for proliferation/survival and found a strong correlation between increased gene copy number and d...

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Veröffentlicht in:	Cancer discovery 2016-08, Vol.6 (8), p.914-929
Hauptverfasser:	Aguirre, Andrew J, Meyers, Robin M, Weir, Barbara A, Vazquez, Francisca, Zhang, Cheng-Zhong, Ben-David, Uri, Cook, April, Ha, Gavin, Harrington, William F, Doshi, Mihir B, Kost-Alimova, Maria, Gill, Stanley, Xu, Han, Ali, Levi D, Jiang, Guozhi, Pantel, Sasha, Lee, Yenarae, Goodale, Amy, Cherniack, Andrew D, Oh, Coyin, Kryukov, Gregory, Cowley, Glenn S, Garraway, Levi A, Stegmaier, Kimberly, Roberts, Charles W, Golub, Todd R, Meyerson, Matthew, Root, David E, Tsherniak, Aviad, Hahn, William C
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems DNA Cleavage DNA Copy Number Variations DNA Damage G2 Phase Cell Cycle Checkpoints Gene Amplification Gene Dosage Gene Editing Gene Expression Gene Knockout Techniques Gene Targeting - methods Genes, Essential Genomics - methods High-Throughput Screening Assays Humans RNA, Guide, CRISPR-Cas Systems
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