A simple modification of PCR thermal profile applied to evade persisting contamination

The polymerase chain reaction (PCR), one of the most commonly applied methods of diagnostics and molecular biology has a frustrating downside known as the false positive signal or contamination. Several solutions to avoid and to eliminate PCR contaminations have been worked out to date but the imple...

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Veröffentlicht in:	Journal of applied genetics 2016-08, Vol.57 (3), p.409-415
Hauptverfasser:	Banasik, Michał, Stanisławska-Sachadyn, Anna, Sachadyn, Paweł
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Annealing Biomedical and Life Sciences Denaturation DNA Contamination False Positive Reactions Human Genetics Life Sciences Microbial Genetics and Genomics Microbial Genetics • Original Paper Molecular biology Plant Genetics and Genomics Polymerase Chain Reaction - methods Proteins Temperature
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