Optical coherence contrast imaging using gold nanorods in living mice eyes
Background Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scatterin...
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| Veröffentlicht in: | Clinical & experimental ophthalmology 2015-05, Vol.43 (4), p.358-366 |
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| container_title | Clinical & experimental ophthalmology |
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| creator | de la Zerda, Adam Prabhulkar, Shradha Perez, Victor L Ruggeri, Marco Paranjape, Amit S Habte, Frezghi Gambhir, Sanjiv S Awdeh, Richard M |
| description | Background
Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.
Methods
Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.
Results
A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50‐fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.
Conclusions
The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high‐resolution molecular imaging modality. |
| doi_str_mv | 10.1111/ceo.12299 |
| format | Article |
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Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.
Methods
Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.
Results
A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50‐fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.
Conclusions
The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high‐resolution molecular imaging modality.</description><identifier>ISSN: 1442-6404</identifier><identifier>EISSN: 1442-9071</identifier><identifier>DOI: 10.1111/ceo.12299</identifier><identifier>PMID: 24533647</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Anatomy, Cross-Sectional ; Animals ; Anterior Chamber - anatomy & histology ; contrast agent ; Contrast Media - chemistry ; Cornea - anatomy & histology ; Gold - chemistry ; gold nanorods ; Imaging, Three-Dimensional ; Mice ; Mice, Inbred C57BL ; molecular imaging ; Nanotubes - chemistry ; ophthalmic imaging ; optical coherence tomography ; Phantoms, Imaging ; Tomography, Optical Coherence - methods</subject><ispartof>Clinical & experimental ophthalmology, 2015-05, Vol.43 (4), p.358-366</ispartof><rights>2014 Royal Australian and New Zealand College of Ophthalmologists</rights><rights>2014 Royal Australian and New Zealand College of Ophthalmologists.</rights><rights>Copyright © 2015 Royal Australian and New Zealand College of Ophthalmologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5519-843a2812fc79c17e55c64388ff77ad6419a16245d698d1d17d9c2f305d169b863</citedby><cites>FETCH-LOGICAL-c5519-843a2812fc79c17e55c64388ff77ad6419a16245d698d1d17d9c2f305d169b863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fceo.12299$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fceo.12299$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24533647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de la Zerda, Adam</creatorcontrib><creatorcontrib>Prabhulkar, Shradha</creatorcontrib><creatorcontrib>Perez, Victor L</creatorcontrib><creatorcontrib>Ruggeri, Marco</creatorcontrib><creatorcontrib>Paranjape, Amit S</creatorcontrib><creatorcontrib>Habte, Frezghi</creatorcontrib><creatorcontrib>Gambhir, Sanjiv S</creatorcontrib><creatorcontrib>Awdeh, Richard M</creatorcontrib><title>Optical coherence contrast imaging using gold nanorods in living mice eyes</title><title>Clinical & experimental ophthalmology</title><addtitle>Clin Experiment Ophthalmol</addtitle><description>Background
Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.
Methods
Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.
Results
A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50‐fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.
Conclusions
The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high‐resolution molecular imaging modality.</description><subject>Anatomy, Cross-Sectional</subject><subject>Animals</subject><subject>Anterior Chamber - anatomy & histology</subject><subject>contrast agent</subject><subject>Contrast Media - chemistry</subject><subject>Cornea - anatomy & histology</subject><subject>Gold - chemistry</subject><subject>gold nanorods</subject><subject>Imaging, Three-Dimensional</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>molecular imaging</subject><subject>Nanotubes - chemistry</subject><subject>ophthalmic imaging</subject><subject>optical coherence tomography</subject><subject>Phantoms, Imaging</subject><subject>Tomography, Optical Coherence - methods</subject><issn>1442-6404</issn><issn>1442-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtrGzEUhUVpaB7ton-gDHTVxSS6eo42gWBSJ6mpCbQUuhGKpHGUjEeuNE7jfx-5dkyyqBbS5eq75x4OQh8BH0M5J9bHYyBEqTfoABgjtcIS3m5rwTDbR4c532GMOaHiHdonjFMqmDxAV9PFEKzpKhtvffK99aXqh2TyUIW5mYV-Vi3z-p7FzlW96WOKLlehr7rwsO7PQ5nxK5_fo73WdNl_2L5H6OfX8x-ji3oyHV-Ozia15RxU3TBqSAOktVJZkJ5zKxhtmraV0jjBQBkQxaATqnHgQDplSUsxdyDUTSPoETrd6C6WN3PvrF_b7fQiFb9ppaMJ-vVPH271LD5opgQFjovA561Ain-WPg_6Li5TXzzrsgITzClAob5sKJtizsm3uw2A9Tp2XWLX_2Iv7KeXlnbkc84FONkAf0PnV_9X0qPz6bNkvZkIefCPuwmT7rWQVHL96_tY_55cfLu-Ggt9TZ8Ait6bVQ</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>de la Zerda, Adam</creator><creator>Prabhulkar, Shradha</creator><creator>Perez, Victor L</creator><creator>Ruggeri, Marco</creator><creator>Paranjape, Amit S</creator><creator>Habte, Frezghi</creator><creator>Gambhir, Sanjiv S</creator><creator>Awdeh, Richard M</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>K9.</scope><scope>5PM</scope></search><sort><creationdate>201505</creationdate><title>Optical coherence contrast imaging using gold nanorods in living mice eyes</title><author>de la Zerda, Adam ; Prabhulkar, Shradha ; Perez, Victor L ; Ruggeri, Marco ; Paranjape, Amit S ; Habte, Frezghi ; Gambhir, Sanjiv S ; Awdeh, Richard M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5519-843a2812fc79c17e55c64388ff77ad6419a16245d698d1d17d9c2f305d169b863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anatomy, Cross-Sectional</topic><topic>Animals</topic><topic>Anterior Chamber - anatomy & histology</topic><topic>contrast agent</topic><topic>Contrast Media - chemistry</topic><topic>Cornea - anatomy & histology</topic><topic>Gold - chemistry</topic><topic>gold nanorods</topic><topic>Imaging, Three-Dimensional</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>molecular imaging</topic><topic>Nanotubes - chemistry</topic><topic>ophthalmic imaging</topic><topic>optical coherence tomography</topic><topic>Phantoms, Imaging</topic><topic>Tomography, Optical Coherence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de la Zerda, Adam</creatorcontrib><creatorcontrib>Prabhulkar, Shradha</creatorcontrib><creatorcontrib>Perez, Victor L</creatorcontrib><creatorcontrib>Ruggeri, Marco</creatorcontrib><creatorcontrib>Paranjape, Amit S</creatorcontrib><creatorcontrib>Habte, Frezghi</creatorcontrib><creatorcontrib>Gambhir, Sanjiv S</creatorcontrib><creatorcontrib>Awdeh, Richard M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical & experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de la Zerda, Adam</au><au>Prabhulkar, Shradha</au><au>Perez, Victor L</au><au>Ruggeri, Marco</au><au>Paranjape, Amit S</au><au>Habte, Frezghi</au><au>Gambhir, Sanjiv S</au><au>Awdeh, Richard M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical coherence contrast imaging using gold nanorods in living mice eyes</atitle><jtitle>Clinical & experimental ophthalmology</jtitle><addtitle>Clin Experiment Ophthalmol</addtitle><date>2015-05</date><risdate>2015</risdate><volume>43</volume><issue>4</issue><spage>358</spage><epage>366</epage><pages>358-366</pages><issn>1442-6404</issn><eissn>1442-9071</eissn><abstract>Background
Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.
Methods
Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.
Results
A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50‐fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.
Conclusions
The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high‐resolution molecular imaging modality.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>24533647</pmid><doi>10.1111/ceo.12299</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anatomy, Cross-Sectional Animals Anterior Chamber - anatomy & histology contrast agent Contrast Media - chemistry Cornea - anatomy & histology Gold - chemistry gold nanorods Imaging, Three-Dimensional Mice Mice, Inbred C57BL molecular imaging Nanotubes - chemistry ophthalmic imaging optical coherence tomography Phantoms, Imaging Tomography, Optical Coherence - methods |
| title | Optical coherence contrast imaging using gold nanorods in living mice eyes |
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