Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
This work describes that cytotoxicity of lead chloride and lead acetate to cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT)...
Gespeichert in:
| Veröffentlicht in: | Interdisciplinary toxicology 2015-03, Vol.8 (1), p.35-43 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 43 |
|---|---|
| container_issue | 1 |
| container_start_page | 35 |
| container_title | Interdisciplinary toxicology |
| container_volume | 8 |
| creator | Patnaik, Rajashree Padhy, Rabindra N. |
| description | This work describes that cytotoxicity of lead chloride and lead acetate to
cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl
. Lethal concentration
(LC
) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC
values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC
values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC
values were almost in the range: 295.12 to 371.53 mg/L; LC
values were in the range: 501.18 to 588.84 mg/L; LC
value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC
values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes. |
| doi_str_mv | 10.1515/intox-2015-0007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4961924</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1809047768</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3885-b1b50642d2bb264a5aa865b192b2f6810cf935c0df2de5450f6052b4704318763</originalsourceid><addsrcrecordid>eNptkU1rFTEUhgdRbKldu5OAGzdj8zHJZBAEKX5BQRe6Dicf05uSmVyTzK3zM_zH5vZeSxVXCSfPed-T8zbNc4JfE074hZ9L_NlSTHiLMe4fNaeEsb4duBgeH-9CcnbSnOd8UwnMCJedeNqc0L6TgnF52vz6mqL2BcEMYc0-ozgiE6ctJCh-5xDkDGutzqh6eeOLd3dMcGCR2YSYvK3UbA8VMK5AcRVGfkY7X1JEZgllSc6izTLBjJZJ--ANhOqTLNIhxtq8TttNNGtx-VnzZISQ3fnxPGu-f3j_7fJTe_Xl4-fLd1etYVLyVhPNseiopVpT0QEHkIJrMlBNRyEJNuPAuMF2pNbxjuNRYE511-OOEdkLdta8PehuFz05a9xcEgS1TX6CtKoIXv39MvuNuo471Q2iunRV4NVRIMUfi8tFTT4bFwLMLi5ZEYkH3PW9kBV9-Q96E5dUV76nBKlp9oRW6uJAmRRzTm68H4ZgtU9c3SWu9omrfeK148XDP9zzf_KtwJsDcAuhuGTddVrqktMD__9LS8I4-w37mL5u</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1861151712</pqid></control><display><type>article</type><title>Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes</title><source>De Gruyter Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Patnaik, Rajashree ; Padhy, Rabindra N.</creator><creatorcontrib>Patnaik, Rajashree ; Padhy, Rabindra N.</creatorcontrib><description>This work describes that cytotoxicity of lead chloride and lead acetate to
cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl
. Lethal concentration
(LC
) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC
values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC
values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC
values were almost in the range: 295.12 to 371.53 mg/L; LC
values were in the range: 501.18 to 588.84 mg/L; LC
value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC
values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes.</description><identifier>ISSN: 1337-6853</identifier><identifier>ISSN: 1337-9569</identifier><identifier>EISSN: 1337-9569</identifier><identifier>DOI: 10.1515/intox-2015-0007</identifier><identifier>PMID: 27486358</identifier><language>eng</language><publisher>Slovakia: De Gruyter Open</publisher><subject>AO/EB staining ; Blood ; Cell culture ; Chloride ; comet assay ; Comparative analysis ; cytotoxicity ; genotoxicity ; Lead ; lead acetate ; lead chloride ; Lymphocytes ; MTT assay ; Original ; Toxicity ; Umbilical cord</subject><ispartof>Interdisciplinary toxicology, 2015-03, Vol.8 (1), p.35-43</ispartof><rights>Copyright De Gruyter Open Sp. z o.o. 2015</rights><rights>Copyright © 2015 SETOX & Institute of Experimental Pharmacology and Toxicology, SASc. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3885-b1b50642d2bb264a5aa865b192b2f6810cf935c0df2de5450f6052b4704318763</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961924/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961924/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768,66901,68685</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27486358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Patnaik, Rajashree</creatorcontrib><creatorcontrib>Padhy, Rabindra N.</creatorcontrib><title>Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes</title><title>Interdisciplinary toxicology</title><addtitle>Interdiscip Toxicol</addtitle><description>This work describes that cytotoxicity of lead chloride and lead acetate to
cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl
. Lethal concentration
(LC
) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC
values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC
values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC
values were almost in the range: 295.12 to 371.53 mg/L; LC
values were in the range: 501.18 to 588.84 mg/L; LC
value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC
values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes.</description><subject>AO/EB staining</subject><subject>Blood</subject><subject>Cell culture</subject><subject>Chloride</subject><subject>comet assay</subject><subject>Comparative analysis</subject><subject>cytotoxicity</subject><subject>genotoxicity</subject><subject>Lead</subject><subject>lead acetate</subject><subject>lead chloride</subject><subject>Lymphocytes</subject><subject>MTT assay</subject><subject>Original</subject><subject>Toxicity</subject><subject>Umbilical cord</subject><issn>1337-6853</issn><issn>1337-9569</issn><issn>1337-9569</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkU1rFTEUhgdRbKldu5OAGzdj8zHJZBAEKX5BQRe6Dicf05uSmVyTzK3zM_zH5vZeSxVXCSfPed-T8zbNc4JfE074hZ9L_NlSTHiLMe4fNaeEsb4duBgeH-9CcnbSnOd8UwnMCJedeNqc0L6TgnF52vz6mqL2BcEMYc0-ozgiE6ctJCh-5xDkDGutzqh6eeOLd3dMcGCR2YSYvK3UbA8VMK5AcRVGfkY7X1JEZgllSc6izTLBjJZJ--ANhOqTLNIhxtq8TttNNGtx-VnzZISQ3fnxPGu-f3j_7fJTe_Xl4-fLd1etYVLyVhPNseiopVpT0QEHkIJrMlBNRyEJNuPAuMF2pNbxjuNRYE511-OOEdkLdta8PehuFz05a9xcEgS1TX6CtKoIXv39MvuNuo471Q2iunRV4NVRIMUfi8tFTT4bFwLMLi5ZEYkH3PW9kBV9-Q96E5dUV76nBKlp9oRW6uJAmRRzTm68H4ZgtU9c3SWu9omrfeK148XDP9zzf_KtwJsDcAuhuGTddVrqktMD__9LS8I4-w37mL5u</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Patnaik, Rajashree</creator><creator>Padhy, Rabindra N.</creator><general>De Gruyter Open</general><general>De Gruyter Poland</general><general>Slovak Toxicology Society SETOX</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150301</creationdate><title>Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes</title><author>Patnaik, Rajashree ; Padhy, Rabindra N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3885-b1b50642d2bb264a5aa865b192b2f6810cf935c0df2de5450f6052b4704318763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>AO/EB staining</topic><topic>Blood</topic><topic>Cell culture</topic><topic>Chloride</topic><topic>comet assay</topic><topic>Comparative analysis</topic><topic>cytotoxicity</topic><topic>genotoxicity</topic><topic>Lead</topic><topic>lead acetate</topic><topic>lead chloride</topic><topic>Lymphocytes</topic><topic>MTT assay</topic><topic>Original</topic><topic>Toxicity</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Patnaik, Rajashree</creatorcontrib><creatorcontrib>Padhy, Rabindra N.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Interdisciplinary toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Patnaik, Rajashree</au><au>Padhy, Rabindra N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes</atitle><jtitle>Interdisciplinary toxicology</jtitle><addtitle>Interdiscip Toxicol</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>8</volume><issue>1</issue><spage>35</spage><epage>43</epage><pages>35-43</pages><issn>1337-6853</issn><issn>1337-9569</issn><eissn>1337-9569</eissn><abstract>This work describes that cytotoxicity of lead chloride and lead acetate to
cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl
. Lethal concentration
(LC
) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC
values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC
values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC
values were almost in the range: 295.12 to 371.53 mg/L; LC
values were in the range: 501.18 to 588.84 mg/L; LC
value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC
values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes.</abstract><cop>Slovakia</cop><pub>De Gruyter Open</pub><pmid>27486358</pmid><doi>10.1515/intox-2015-0007</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1337-6853 |
| ispartof | Interdisciplinary toxicology, 2015-03, Vol.8 (1), p.35-43 |
| issn | 1337-6853 1337-9569 1337-9569 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4961924 |
| source | De Gruyter Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | AO/EB staining Blood Cell culture Chloride comet assay Comparative analysis cytotoxicity genotoxicity Lead lead acetate lead chloride Lymphocytes MTT assay Original Toxicity Umbilical cord |
| title | Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T12%3A54%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Probit%20analysis%20of%20comparative%20assays%20on%20toxicities%20of%20lead%20chloride%20and%20lead%20acetate%20to%20in%20vitro%20cultured%20human%20umbilical%20cord%20blood%20lymphocytes&rft.jtitle=Interdisciplinary%20toxicology&rft.au=Patnaik,%20Rajashree&rft.date=2015-03-01&rft.volume=8&rft.issue=1&rft.spage=35&rft.epage=43&rft.pages=35-43&rft.issn=1337-6853&rft.eissn=1337-9569&rft_id=info:doi/10.1515/intox-2015-0007&rft_dat=%3Cproquest_pubme%3E1809047768%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1861151712&rft_id=info:pmid/27486358&rfr_iscdi=true |