High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells

[Display omitted] Non-viral gene-editing of human cells using the CRISPR-Cas9 system requires optimized delivery of multiple components. Both the Cas9 endonuclease and a single guide RNA, that defines the genomic target, need to be present and co-localized within the nucleus for efficient gene-editi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Acta biomaterialia 2016-04, Vol.34, p.143-158
Hauptverfasser:	Steyer, Benjamin, Carlson-Stevermer, Jared, Angenent-Mari, Nicolas, Khalil, Andrew, Harkness, Ty, Saha, Krishanu
Format:	Artikel
Sprache:	eng
Schlagworte:	Cationic lipids CRISPR-Cas Systems - genetics CRISPR-Cas9 Design engineering Design of experiments DNA/RNA Flow Cytometry Fluorescence Gene Editing Gene Transfer Techniques Genes, Reporter Genetic modification Genomes HEK293 Cells High content analysis High-Throughput Screening Assays - methods Human Human embryonic cells Humans Lipids - chemistry Multivariate Analysis Optimization Platforms Reference Standards Ribonucleic acids RNA, Guide, CRISPR-Cas Systems - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Strategy Time Factors Transcription, Genetic Transgenes
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!