Self-assembly Controls Self-cleavage of HHR from ASBVd (−): a Combined SANS and Modeling Study
In the Avocado Sunblotch Viroid (ASBVd: 249-nt) from the Avsunviroidae family, a symmetric rolling-circle replication operates through an autocatalytic mechanism mediated by hammerhead ribozymes (HHR) embedded in both polarity strands. The concatenated multimeric ASBVd (+) and ASBVd (−) RNAs thus ge...
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| Veröffentlicht in: | Scientific reports 2016-07, Vol.6 (1), p.30287-30287, Article 30287 |
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| creator | Leclerc, Fabrice Zaccai, Giuseppe Vergne, Jacques Řìhovà, Martina Martel, Anne Maurel, Marie-Christine |
| description | In the Avocado Sunblotch Viroid (ASBVd: 249-nt) from the Avsunviroidae family, a symmetric rolling-circle replication operates through an autocatalytic mechanism mediated by hammerhead ribozymes (HHR) embedded in both polarity strands. The concatenated multimeric ASBVd (+) and ASBVd (−) RNAs thus generated are processed by cleavage to unit-length where ASBVd (−) self-cleaves with more efficiency. Absolute scale small angle neutron scattering (SANS) revealed a temperature-dependent dimer association in both ASBVd (−) and its derived 79-nt HHR (−). A joint thermodynamic analysis of SANS and catalytic data indicates the rate-determining step corresponds to the dimer/monomer transition. 2D and 3D models of monomeric and dimeric HHR (−) suggest that the inter-molecular contacts stabilizing the dimer (between HI and HII domains) compete with the intra-molecular ones stabilizing the active conformation of the full-length HHR required for an efficient self-cleavage. Similar competing intra- and inter-molecular contacts are proposed in ASBVd (−) though with a remoter region from an extension of the HI domain. |
| doi_str_mv | 10.1038/srep30287 |
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| subjects | 119/118 631/114/2397 631/337/1910 631/45/607/1171 631/535/1267 631/57/2272/951 Cellular Biology Humanities and Social Sciences Life Sciences multidisciplinary Science |
| title | Self-assembly Controls Self-cleavage of HHR from ASBVd (−): a Combined SANS and Modeling Study |
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