Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes
Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRN...
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| Veröffentlicht in: | Applied and environmental microbiology 2016-04, Vol.82 (8), p.2263-2269 |
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| creator | Kreuzer-Redmer, Susanne Bekurtz, Jennifer C Arends, Danny Bortfeldt, Ralf Kutz-Lohroff, Barbara Sharbati, Soroush Einspanier, Ralf Brockmann, Gudrun A |
| description | Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of E. faeciumon immune cell regulation in the small intestine. |
| doi_str_mv | 10.1128/AEM.04044-15 |
| format | Article |
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One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of E. faeciumon immune cell regulation in the small intestine.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.04044-15</identifier><identifier>PMID: 26826223</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Animals ; Animals, Newborn ; Down-Regulation ; Enterococcus faecium - immunology ; Food Microbiology ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genes ; Immunoglobulins ; Immunoglobulins - metabolism ; MicroRNAs - metabolism ; Polymerase chain reaction ; Probiotics ; Real-Time Polymerase Chain Reaction ; Swine ; Tissues</subject><ispartof>Applied and environmental microbiology, 2016-04, Vol.82 (8), p.2263-2269</ispartof><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright American Society for Microbiology Apr 2016</rights><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved. 2016 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-e06f90d67be3f771c869ea2b0c85fa5ef85388d35a413c9911b158472a333de93</citedby><cites>FETCH-LOGICAL-c342t-e06f90d67be3f771c869ea2b0c85fa5ef85388d35a413c9911b158472a333de93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959498/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959498/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,3175,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26826223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Björkroth, J.</contributor><creatorcontrib>Kreuzer-Redmer, Susanne</creatorcontrib><creatorcontrib>Bekurtz, Jennifer C</creatorcontrib><creatorcontrib>Arends, Danny</creatorcontrib><creatorcontrib>Bortfeldt, Ralf</creatorcontrib><creatorcontrib>Kutz-Lohroff, Barbara</creatorcontrib><creatorcontrib>Sharbati, Soroush</creatorcontrib><creatorcontrib>Einspanier, Ralf</creatorcontrib><creatorcontrib>Brockmann, Gudrun A</creatorcontrib><title>Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of E. faeciumon immune cell regulation in the small intestine.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Down-Regulation</subject><subject>Enterococcus faecium - immunology</subject><subject>Food Microbiology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Immunoglobulins</subject><subject>Immunoglobulins - metabolism</subject><subject>MicroRNAs - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Probiotics</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Swine</subject><subject>Tissues</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9v0zAYQC3ExLrBjTOyxIXDPPwzsS9IXdWNSN2QKjhbrvOleErsEicT---XsDHBTj746dnf9xB6z-g5Y1x_Xq6vz6mkUhKmXqEFo0YTJUTxGi0oNYZwLukxOsn5ltKJK_QbdMwLzQvOxQL9vgSoQ9zj1OB1HKBPPnk_Ztw48GHs8M2qur7AjEqm8AZcnfGQcDWReQjRtbgL25slkVwQdSBVrEcPNd7CfmzdEFKcvVXXjRHIFlq4c3HAVxAhv0VHjWszvHs6T9GPy_X31Vey-XZVrZYb4oXkAwFaNIbWRbkD0ZQl87ow4PiOeq0ap6DRSmhdC-UkE94YxnZMaVlyJ4SowYhT9OXRexh3HdQe4tC71h760Ln-3iYX7P83Mfy0-3RnpVFGGj0JPj0J-vRrnMa2Xcge2tZFSGO2rCwN5VyV81sfX6C3aeynLc2UnkJopWfh2SPl-5RzD83zZxi1c1I7JbV_klqmJvzDvwM8w38bigd1T5oX</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Kreuzer-Redmer, Susanne</creator><creator>Bekurtz, Jennifer C</creator><creator>Arends, Danny</creator><creator>Bortfeldt, Ralf</creator><creator>Kutz-Lohroff, Barbara</creator><creator>Sharbati, Soroush</creator><creator>Einspanier, Ralf</creator><creator>Brockmann, Gudrun A</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160401</creationdate><title>Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes</title><author>Kreuzer-Redmer, Susanne ; 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One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from Enterococcus faeciumNCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold;P= 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold;P= 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold;P= 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold;P= 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anti correlated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of E. faeciumon the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of E. faeciumon immune cell regulation in the small intestine.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>26826223</pmid><doi>10.1128/AEM.04044-15</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Newborn Down-Regulation Enterococcus faecium - immunology Food Microbiology Gene expression Gene Expression Profiling Gene Expression Regulation Genes Immunoglobulins Immunoglobulins - metabolism MicroRNAs - metabolism Polymerase chain reaction Probiotics Real-Time Polymerase Chain Reaction Swine Tissues |
| title | Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes |
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