Cell Culture Isolation of Piscine Nodavirus (Betanodavirus) in Fish-Rearing Seawater

Piscine nodavirus (betanodavirus) is the causative agent of viral nervous necrosis (VNN) in a variety of cultured fish species, particularly marine fish. In the present study, we developed a sensitive method for cell culture isolation of the virus from seawater and applied the method to a spontaneou...

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Veröffentlicht in:	Applied and environmental microbiology 2016-04, Vol.82 (8), p.2537-2544
Hauptverfasser:	Nishi, Shinnosuke, Yamashita, Hirofumi, Kawato, Yasuhiko, Nakai, Toshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aquaculture Bass - virology Cell culture Fish Fish Diseases - virology Flocculation Gangrene Methods Nodaviridae - growth & development Nodaviridae - isolation & purification Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA Virus Infections - virology RNA, Viral - genetics Seawater Seawater - virology Sequence Analysis, DNA Virus Cultivation
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