New rapid identification test for Clostridium difficile
AIMS: A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS: The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopep...
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| Veröffentlicht in: | Journal of clinical pathology 1992-11, Vol.45 (11), p.956-958 |
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| creator | Aspinall, S T Dealler, S F |
| description | AIMS: A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS: The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopeptidase, galactosidase, leucine aminopeptidase, acid phosphatase and indole). The strips were inoculated, incubated for 20 minutes, and reagents added. RESULTS: 96.4% (212 of 220) strains of C difficile were immediately differentiated from 51 other Clostridium spp tested. The remaining 3.6% (eight of 220) of C difficile isolates produced a reaction pattern similar to some of the Clostridium sporogenes tested and required additional tests. None of the other Clostridium spp tested produced reaction patterns similar to C difficile. CONCLUSION: The Diffstrip allowed colonies of C difficile to be confirmed within 30 minutes for 96.4% of isolates, with less than 4% requiring any additional tests. No strains of C difficile were misidentified and no strains of other Clostridium spp tested were misidentified as C difficile. |
| doi_str_mv | 10.1136/jcp.45.11.956 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_495023</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3971356491</sourcerecordid><originalsourceid>FETCH-LOGICAL-b511t-823dcbcfb9bdd0ba90bdd19c1c2156ca78283e06ba9f38494fd7aa3a4a0394973</originalsourceid><addsrcrecordid>eNqFkUtP3DAUhS1UBFPKkiVSpEqom0x941e86KIaUUDAVEK0CzaWYzvU0ySe2kkL_75Gg4B2w-pe6Xz3eRA6ADwHIPzjyqznlOV8LhnfQjOgoiopUP4GzTCuoJSC8l30NqUVxkAEkB20A5RVoq5nSCzdnyLqtbeFt24YfeuNHn0YitGlsWhDLBZdSGP01k99YX2bAd-5d2i71V1y-49xD337cny9OC0vvp6cLT5flA0DGMu6ItY0pm1kYy1utMQ5gjRgKmDcaFFXNXGYZ6UlNZW0tUJroqnGRFIpyB76tOm7npreWZNXjLpT6-h7He9V0F79qwz-h7oNvxWVDFck1x891sfwa8onqd4n47pODy5MSQlCMQeGXwWB17WosMzg-__AVZjikJ-gQAhMGeaCZ6rcUCaGlKJrn1YGrB58U9k3RVnOVfYt84cv73ymN0Y99_NpdHdPso4_FRdEMLX8vlA37IqTy9Nztcz8hw3f9KtXRv8FskCwDg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770450676</pqid></control><display><type>article</type><title>New rapid identification test for Clostridium difficile</title><source>PubMed Central (Open Access)</source><source>MEDLINE</source><source>Free E-Journal (出版社公開部分のみ）</source><source>Alma/SFX Local Collection</source><creator>Aspinall, S T ; Dealler, S F</creator><creatorcontrib>Aspinall, S T ; Dealler, S F</creatorcontrib><description>AIMS: A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS: The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopeptidase, galactosidase, leucine aminopeptidase, acid phosphatase and indole). The strips were inoculated, incubated for 20 minutes, and reagents added. RESULTS: 96.4% (212 of 220) strains of C difficile were immediately differentiated from 51 other Clostridium spp tested. The remaining 3.6% (eight of 220) of C difficile isolates produced a reaction pattern similar to some of the Clostridium sporogenes tested and required additional tests. None of the other Clostridium spp tested produced reaction patterns similar to C difficile. CONCLUSION: The Diffstrip allowed colonies of C difficile to be confirmed within 30 minutes for 96.4% of isolates, with less than 4% requiring any additional tests. No strains of C difficile were misidentified and no strains of other Clostridium spp tested were misidentified as C difficile.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.45.11.956</identifier><identifier>PMID: 1452788</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>England: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Bacterial Typing Techniques ; Bacteriological Techniques ; Clostridium difficile ; Clostridium difficile - classification ; Clostridium difficile - isolation & purification ; Humans ; Reagent Strips ; Species Specificity ; Substrate Specificity ; Time Factors</subject><ispartof>Journal of clinical pathology, 1992-11, Vol.45 (11), p.956-958</ispartof><rights>Copyright BMJ Publishing Group LTD Nov 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b511t-823dcbcfb9bdd0ba90bdd19c1c2156ca78283e06ba9f38494fd7aa3a4a0394973</citedby><cites>FETCH-LOGICAL-b511t-823dcbcfb9bdd0ba90bdd19c1c2156ca78283e06ba9f38494fd7aa3a4a0394973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC495023/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC495023/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1452788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aspinall, S T</creatorcontrib><creatorcontrib>Dealler, S F</creatorcontrib><title>New rapid identification test for Clostridium difficile</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>AIMS: A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS: The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopeptidase, galactosidase, leucine aminopeptidase, acid phosphatase and indole). The strips were inoculated, incubated for 20 minutes, and reagents added. RESULTS: 96.4% (212 of 220) strains of C difficile were immediately differentiated from 51 other Clostridium spp tested. The remaining 3.6% (eight of 220) of C difficile isolates produced a reaction pattern similar to some of the Clostridium sporogenes tested and required additional tests. None of the other Clostridium spp tested produced reaction patterns similar to C difficile. CONCLUSION: The Diffstrip allowed colonies of C difficile to be confirmed within 30 minutes for 96.4% of isolates, with less than 4% requiring any additional tests. No strains of C difficile were misidentified and no strains of other Clostridium spp tested were misidentified as C difficile.</description><subject>Bacterial Typing Techniques</subject><subject>Bacteriological Techniques</subject><subject>Clostridium difficile</subject><subject>Clostridium difficile - classification</subject><subject>Clostridium difficile - isolation & purification</subject><subject>Humans</subject><subject>Reagent Strips</subject><subject>Species Specificity</subject><subject>Substrate Specificity</subject><subject>Time Factors</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUtP3DAUhS1UBFPKkiVSpEqom0x941e86KIaUUDAVEK0CzaWYzvU0ySe2kkL_75Gg4B2w-pe6Xz3eRA6ADwHIPzjyqznlOV8LhnfQjOgoiopUP4GzTCuoJSC8l30NqUVxkAEkB20A5RVoq5nSCzdnyLqtbeFt24YfeuNHn0YitGlsWhDLBZdSGP01k99YX2bAd-5d2i71V1y-49xD337cny9OC0vvp6cLT5flA0DGMu6ItY0pm1kYy1utMQ5gjRgKmDcaFFXNXGYZ6UlNZW0tUJroqnGRFIpyB76tOm7npreWZNXjLpT6-h7He9V0F79qwz-h7oNvxWVDFck1x891sfwa8onqd4n47pODy5MSQlCMQeGXwWB17WosMzg-__AVZjikJ-gQAhMGeaCZ6rcUCaGlKJrn1YGrB58U9k3RVnOVfYt84cv73ymN0Y99_NpdHdPso4_FRdEMLX8vlA37IqTy9Nztcz8hw3f9KtXRv8FskCwDg</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Aspinall, S T</creator><creator>Dealler, S F</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19921101</creationdate><title>New rapid identification test for Clostridium difficile</title><author>Aspinall, S T ; Dealler, S F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b511t-823dcbcfb9bdd0ba90bdd19c1c2156ca78283e06ba9f38494fd7aa3a4a0394973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacterial Typing Techniques</topic><topic>Bacteriological Techniques</topic><topic>Clostridium difficile</topic><topic>Clostridium difficile - classification</topic><topic>Clostridium difficile - isolation & purification</topic><topic>Humans</topic><topic>Reagent Strips</topic><topic>Species Specificity</topic><topic>Substrate Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aspinall, S T</creatorcontrib><creatorcontrib>Dealler, S F</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aspinall, S T</au><au>Dealler, S F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New rapid identification test for Clostridium difficile</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>45</volume><issue>11</issue><spage>956</spage><epage>958</epage><pages>956-958</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>AIMS: A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS: The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopeptidase, galactosidase, leucine aminopeptidase, acid phosphatase and indole). The strips were inoculated, incubated for 20 minutes, and reagents added. RESULTS: 96.4% (212 of 220) strains of C difficile were immediately differentiated from 51 other Clostridium spp tested. The remaining 3.6% (eight of 220) of C difficile isolates produced a reaction pattern similar to some of the Clostridium sporogenes tested and required additional tests. None of the other Clostridium spp tested produced reaction patterns similar to C difficile. CONCLUSION: The Diffstrip allowed colonies of C difficile to be confirmed within 30 minutes for 96.4% of isolates, with less than 4% requiring any additional tests. No strains of C difficile were misidentified and no strains of other Clostridium spp tested were misidentified as C difficile.</abstract><cop>England</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>1452788</pmid><doi>10.1136/jcp.45.11.956</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central (Open Access); MEDLINE; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Bacterial Typing Techniques Bacteriological Techniques Clostridium difficile Clostridium difficile - classification Clostridium difficile - isolation & purification Humans Reagent Strips Species Specificity Substrate Specificity Time Factors |
| title | New rapid identification test for Clostridium difficile |
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