Long-term Renewable Human Intestinal Epithelial Stem Cells as Monolayers: A Potential for Clinical Use

Abstract Purpose Current culture schema for human intestinal stem cells (hISCs) frequently rely on a 3D culture system using MatrigelTM , a laminin-rich matrix derived from murine sarcoma that is not suitable for clinical use. We have developed a novel 2D culture system for the in vitro expansion of...

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Veröffentlicht in:	Journal of pediatric surgery 2016-06, Vol.51 (6), p.995-1000
Hauptverfasser:	Scott, Andrew, Rouch, Joshua D, Jabaji, Ziyad, Khalil, Hassan A, Solorzano, Sergio, Lewis, Michael, Martín, Martín G, Stelzner, Matthias G, Dunn, James C.Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Biocompatible Materials Cadaver Cell Culture Techniques Collagen Type I Differentiation Enteroids Epithelial Cells - cytology Human intestinal epithelial stem cells Human laminin Humans In vitro culture In Vitro Techniques Intestinal Mucosa - cytology Laminin Monolayers Pediatrics Spheroids Stem Cells - cytology Surgery Type I collagen
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