Evaluation of the External RNA Controls Consortium (ERCC) reference material using a modified Latin square design

Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appropriate controls. Methods to prototype and evaluate multiple RNA controls were developed as...

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Veröffentlicht in:	BMC biotechnology 2016-06, Vol.16 (1), p.54-54, Article 54
Hauptverfasser:	Pine, P Scott, Munro, Sarah A, Parsons, Jerod R, McDaniel, Jennifer, Lucas, Anne Bergstrom, Lozach, Jean, Myers, Timothy G, Su, Qin, Jacobs-Helber, Sarah M, Salit, Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Consortia Gene expression Gene Expression Profiling - methods Gene Expression Profiling - standards gene expression regulation Genomes High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards Humans Hybridization Latin square design liver medicine messenger RNA Methodology Methods microarray technology mixing Plasmids prototypes Reference materials Reference Values Reproducibility of Results RNA - genetics RNA - standards RNA polymerase RNA sequencing Sensitivity and Specificity Sequence Analysis, RNA - standards Software transcriptomics
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