Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α
Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2 and D3. In Xenopus , only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progen...
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| creator | Lien, Huang-Wei Yuan, Rey-Yue Chou, Chih-Ming Chen, Yi-Chung Hung, Chin-Chun Hu, Chin-Hwa Hwang, Sheng-Ping L. Hwang, Pung-Pung Shen, Chia-Ning Chen, Chih-Lung Cheng, Chia-Hsiung Huang, Chang-Jen |
| description | Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2 and D3. In
Xenopus
, only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progenitors during embryogenesis. It remains unknown whether zebrafish possess cyclin D3 or cyclin Dx. In this study, we identified a zebrafish
ccndx
gene encoding a protein which can form a complex with Cdk4. Through whole-mount
in situ
hybridization, we observed that
zccndx
mRNA is expressed in the motor neurons of hindbrain and spinal cord during development. Analysis of a 4-kb promoter sequence of the
zccndx
gene revealed the presence of HRE sites, which can be regulated by HIF2α. Morpholino knockdown of zebrafish Hif2α and cyclin Dx resulted in the abolishment of
isl1
and
oligo2
expression in the precursors of motor neurons and also disrupted axon growth. Overexpression of cyclin Dx mRNA in Hif2α morphants partially rescued
zccndx
expression. Taken together, our data indicate that zebrafish cyclin Dx plays a role in maintaining the precursors of motor neurons. |
| doi_str_mv | 10.1038/srep28297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4915019</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1798993244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-f84af46a6df87d1c6d84684016ef7d9caeeef6359e4567f32910704170caf4db3</originalsourceid><addsrcrecordid>eNptkc1OHSEYhkmjqUZd9AYaltVkKjDMD5smjT9tExM3dtMNYeDjHMwMjDBjzrmGXk1vxGsSPfZEE9lAvu_h4edF6BMlXykp29MUYWQtE80HtM8IrwpWMrbzar2HjlK6JXlUTHAqPqI91pSsFETso79_oIvKurTEeq175_H5CruEI9zNLoLBNkRs4B76MA7gJxwsHsKUix7mGDweY1iAd7mSsPIGuylhWI0RUnK5_axazL2asqtb4-V6DCunCufNrF3XA7ZKP-nYw79DtGtVn-DoZT5Avy8vbs5-FlfXP36dfb8qNKdkKmzLleW1qo1tG0N1bVpet5zQGmxjhFYAYOuyEsCrurElE5Q0hNOG6LzPdOUB-rbxjnM3gNH5WVH1coxuUHEtg3Lybce7pVyEe8kFrQgVWfDlRRDD3QxpkoNLGvpeeQhzkrQRrRAl4zyjxxtUx5ByVHZ7DCXyKT-5zS-zn1_fa0v-TysDJxsg5ZZfQJS3YY4-_9U7tkdvIam1</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1798993244</pqid></control><display><type>article</type><title>Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Lien, Huang-Wei ; Yuan, Rey-Yue ; Chou, Chih-Ming ; Chen, Yi-Chung ; Hung, Chin-Chun ; Hu, Chin-Hwa ; Hwang, Sheng-Ping L. ; Hwang, Pung-Pung ; Shen, Chia-Ning ; Chen, Chih-Lung ; Cheng, Chia-Hsiung ; Huang, Chang-Jen</creator><creatorcontrib>Lien, Huang-Wei ; Yuan, Rey-Yue ; Chou, Chih-Ming ; Chen, Yi-Chung ; Hung, Chin-Chun ; Hu, Chin-Hwa ; Hwang, Sheng-Ping L. ; Hwang, Pung-Pung ; Shen, Chia-Ning ; Chen, Chih-Lung ; Cheng, Chia-Hsiung ; Huang, Chang-Jen</creatorcontrib><description>Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2 and D3. In
Xenopus
, only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progenitors during embryogenesis. It remains unknown whether zebrafish possess cyclin D3 or cyclin Dx. In this study, we identified a zebrafish
ccndx
gene encoding a protein which can form a complex with Cdk4. Through whole-mount
in situ
hybridization, we observed that
zccndx
mRNA is expressed in the motor neurons of hindbrain and spinal cord during development. Analysis of a 4-kb promoter sequence of the
zccndx
gene revealed the presence of HRE sites, which can be regulated by HIF2α. Morpholino knockdown of zebrafish Hif2α and cyclin Dx resulted in the abolishment of
isl1
and
oligo2
expression in the precursors of motor neurons and also disrupted axon growth. Overexpression of cyclin Dx mRNA in Hif2α morphants partially rescued
zccndx
expression. Taken together, our data indicate that zebrafish cyclin Dx plays a role in maintaining the precursors of motor neurons.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep28297</identifier><identifier>PMID: 27323909</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136/368/2430 ; 631/337/572/2102 ; Animals ; Basic Helix-Loop-Helix Transcription Factors - physiology ; Cell Proliferation ; Cercopithecus aethiops ; COS Cells ; Cyclins - physiology ; Gene Expression ; Gene Expression Regulation, Developmental ; HEK293 Cells ; Humanities and Social Sciences ; Humans ; Mice ; Motor Neurons - physiology ; multidisciplinary ; Neural Stem Cells - physiology ; Neurogenesis ; Science ; Zebrafish - embryology</subject><ispartof>Scientific reports, 2016-06, Vol.6 (1), p.28297-28297, Article 28297</ispartof><rights>The Author(s) 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-f84af46a6df87d1c6d84684016ef7d9caeeef6359e4567f32910704170caf4db3</citedby><cites>FETCH-LOGICAL-c410t-f84af46a6df87d1c6d84684016ef7d9caeeef6359e4567f32910704170caf4db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915019/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915019/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27323909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lien, Huang-Wei</creatorcontrib><creatorcontrib>Yuan, Rey-Yue</creatorcontrib><creatorcontrib>Chou, Chih-Ming</creatorcontrib><creatorcontrib>Chen, Yi-Chung</creatorcontrib><creatorcontrib>Hung, Chin-Chun</creatorcontrib><creatorcontrib>Hu, Chin-Hwa</creatorcontrib><creatorcontrib>Hwang, Sheng-Ping L.</creatorcontrib><creatorcontrib>Hwang, Pung-Pung</creatorcontrib><creatorcontrib>Shen, Chia-Ning</creatorcontrib><creatorcontrib>Chen, Chih-Lung</creatorcontrib><creatorcontrib>Cheng, Chia-Hsiung</creatorcontrib><creatorcontrib>Huang, Chang-Jen</creatorcontrib><title>Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2 and D3. In
Xenopus
, only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progenitors during embryogenesis. It remains unknown whether zebrafish possess cyclin D3 or cyclin Dx. In this study, we identified a zebrafish
ccndx
gene encoding a protein which can form a complex with Cdk4. Through whole-mount
in situ
hybridization, we observed that
zccndx
mRNA is expressed in the motor neurons of hindbrain and spinal cord during development. Analysis of a 4-kb promoter sequence of the
zccndx
gene revealed the presence of HRE sites, which can be regulated by HIF2α. Morpholino knockdown of zebrafish Hif2α and cyclin Dx resulted in the abolishment of
isl1
and
oligo2
expression in the precursors of motor neurons and also disrupted axon growth. Overexpression of cyclin Dx mRNA in Hif2α morphants partially rescued
zccndx
expression. Taken together, our data indicate that zebrafish cyclin Dx plays a role in maintaining the precursors of motor neurons.</description><subject>631/136/368/2430</subject><subject>631/337/572/2102</subject><subject>Animals</subject><subject>Basic Helix-Loop-Helix Transcription Factors - physiology</subject><subject>Cell Proliferation</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Cyclins - physiology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>HEK293 Cells</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Mice</subject><subject>Motor Neurons - physiology</subject><subject>multidisciplinary</subject><subject>Neural Stem Cells - physiology</subject><subject>Neurogenesis</subject><subject>Science</subject><subject>Zebrafish - embryology</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNptkc1OHSEYhkmjqUZd9AYaltVkKjDMD5smjT9tExM3dtMNYeDjHMwMjDBjzrmGXk1vxGsSPfZEE9lAvu_h4edF6BMlXykp29MUYWQtE80HtM8IrwpWMrbzar2HjlK6JXlUTHAqPqI91pSsFETso79_oIvKurTEeq175_H5CruEI9zNLoLBNkRs4B76MA7gJxwsHsKUix7mGDweY1iAd7mSsPIGuylhWI0RUnK5_axazL2asqtb4-V6DCunCufNrF3XA7ZKP-nYw79DtGtVn-DoZT5Avy8vbs5-FlfXP36dfb8qNKdkKmzLleW1qo1tG0N1bVpet5zQGmxjhFYAYOuyEsCrurElE5Q0hNOG6LzPdOUB-rbxjnM3gNH5WVH1coxuUHEtg3Lybce7pVyEe8kFrQgVWfDlRRDD3QxpkoNLGvpeeQhzkrQRrRAl4zyjxxtUx5ByVHZ7DCXyKT-5zS-zn1_fa0v-TysDJxsg5ZZfQJS3YY4-_9U7tkdvIam1</recordid><startdate>20160621</startdate><enddate>20160621</enddate><creator>Lien, Huang-Wei</creator><creator>Yuan, Rey-Yue</creator><creator>Chou, Chih-Ming</creator><creator>Chen, Yi-Chung</creator><creator>Hung, Chin-Chun</creator><creator>Hu, Chin-Hwa</creator><creator>Hwang, Sheng-Ping L.</creator><creator>Hwang, Pung-Pung</creator><creator>Shen, Chia-Ning</creator><creator>Chen, Chih-Lung</creator><creator>Cheng, Chia-Hsiung</creator><creator>Huang, Chang-Jen</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160621</creationdate><title>Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α</title><author>Lien, Huang-Wei ; Yuan, Rey-Yue ; Chou, Chih-Ming ; Chen, Yi-Chung ; Hung, Chin-Chun ; Hu, Chin-Hwa ; Hwang, Sheng-Ping L. ; Hwang, Pung-Pung ; Shen, Chia-Ning ; Chen, Chih-Lung ; Cheng, Chia-Hsiung ; Huang, Chang-Jen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-f84af46a6df87d1c6d84684016ef7d9caeeef6359e4567f32910704170caf4db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/136/368/2430</topic><topic>631/337/572/2102</topic><topic>Animals</topic><topic>Basic Helix-Loop-Helix Transcription Factors - physiology</topic><topic>Cell Proliferation</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Cyclins - physiology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>HEK293 Cells</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Mice</topic><topic>Motor Neurons - physiology</topic><topic>multidisciplinary</topic><topic>Neural Stem Cells - physiology</topic><topic>Neurogenesis</topic><topic>Science</topic><topic>Zebrafish - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lien, Huang-Wei</creatorcontrib><creatorcontrib>Yuan, Rey-Yue</creatorcontrib><creatorcontrib>Chou, Chih-Ming</creatorcontrib><creatorcontrib>Chen, Yi-Chung</creatorcontrib><creatorcontrib>Hung, Chin-Chun</creatorcontrib><creatorcontrib>Hu, Chin-Hwa</creatorcontrib><creatorcontrib>Hwang, Sheng-Ping L.</creatorcontrib><creatorcontrib>Hwang, Pung-Pung</creatorcontrib><creatorcontrib>Shen, Chia-Ning</creatorcontrib><creatorcontrib>Chen, Chih-Lung</creatorcontrib><creatorcontrib>Cheng, Chia-Hsiung</creatorcontrib><creatorcontrib>Huang, Chang-Jen</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lien, Huang-Wei</au><au>Yuan, Rey-Yue</au><au>Chou, Chih-Ming</au><au>Chen, Yi-Chung</au><au>Hung, Chin-Chun</au><au>Hu, Chin-Hwa</au><au>Hwang, Sheng-Ping L.</au><au>Hwang, Pung-Pung</au><au>Shen, Chia-Ning</au><au>Chen, Chih-Lung</au><au>Cheng, Chia-Hsiung</au><au>Huang, Chang-Jen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-06-21</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>28297</spage><epage>28297</epage><pages>28297-28297</pages><artnum>28297</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cyclins play a central role in cell-cycle regulation; in mammals, the D family of cyclins consists of cyclin D1, D2 and D3. In
Xenopus
, only homologs of cyclins D1 and D2 have been reported, while a novel cyclin, cyclin Dx (ccndx), was found to be required for the maintenance of motor neuron progenitors during embryogenesis. It remains unknown whether zebrafish possess cyclin D3 or cyclin Dx. In this study, we identified a zebrafish
ccndx
gene encoding a protein which can form a complex with Cdk4. Through whole-mount
in situ
hybridization, we observed that
zccndx
mRNA is expressed in the motor neurons of hindbrain and spinal cord during development. Analysis of a 4-kb promoter sequence of the
zccndx
gene revealed the presence of HRE sites, which can be regulated by HIF2α. Morpholino knockdown of zebrafish Hif2α and cyclin Dx resulted in the abolishment of
isl1
and
oligo2
expression in the precursors of motor neurons and also disrupted axon growth. Overexpression of cyclin Dx mRNA in Hif2α morphants partially rescued
zccndx
expression. Taken together, our data indicate that zebrafish cyclin Dx plays a role in maintaining the precursors of motor neurons.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27323909</pmid><doi>10.1038/srep28297</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 631/136/368/2430 631/337/572/2102 Animals Basic Helix-Loop-Helix Transcription Factors - physiology Cell Proliferation Cercopithecus aethiops COS Cells Cyclins - physiology Gene Expression Gene Expression Regulation, Developmental HEK293 Cells Humanities and Social Sciences Humans Mice Motor Neurons - physiology multidisciplinary Neural Stem Cells - physiology Neurogenesis Science Zebrafish - embryology |
| title | Zebrafish cyclin Dx is required for development of motor neuron progenitors and its expression is regulated by hypoxia-inducible factor 2α |
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