Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects
Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischem...
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| Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.28250-28250, Article 28250 |
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| creator | Cai, Min Shen, Rui Song, Lei Lu, Minjie Wang, Jianguang Zhao, Shihua Tang, Yue Meng, Xianmin Li, Zongjin He, Zuo-Xiang |
| description | Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway. |
| doi_str_mv | 10.1038/srep28250 |
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In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep28250</identifier><identifier>PMID: 27321050</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 631/532/2074 ; 692/4019/592/2725 ; Animal models ; Bone marrow ; Cardiac function ; Cardiomyocytes ; Cardiovascular disease ; Enzymes ; Glucose ; Heart attacks ; Hospitals ; Humanities and Social Sciences ; Ischemia ; Kinases ; Magnetic resonance imaging ; Medical research ; Metabolism ; multidisciplinary ; Myocardial infarction ; Nuclear medicine ; Protein expression ; Protein synthesis ; Proteins ; Science ; Science (multidisciplinary) ; Signal transduction ; Stem cells ; Tomography ; Transplantation</subject><ispartof>Scientific reports, 2016-06, Vol.6 (1), p.28250-28250, Article 28250</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Jun 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-5bf5b79000fc42fa4b88ac8c5950f489834079ca811f105ec844a76f25aecb393</citedby><cites>FETCH-LOGICAL-c504t-5bf5b79000fc42fa4b88ac8c5950f489834079ca811f105ec844a76f25aecb393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913323/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913323/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27321050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Min</creatorcontrib><creatorcontrib>Shen, Rui</creatorcontrib><creatorcontrib>Song, Lei</creatorcontrib><creatorcontrib>Lu, Minjie</creatorcontrib><creatorcontrib>Wang, Jianguang</creatorcontrib><creatorcontrib>Zhao, Shihua</creatorcontrib><creatorcontrib>Tang, Yue</creatorcontrib><creatorcontrib>Meng, Xianmin</creatorcontrib><creatorcontrib>Li, Zongjin</creatorcontrib><creatorcontrib>He, Zuo-Xiang</creatorcontrib><title>Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway.</description><subject>13/100</subject><subject>631/532/2074</subject><subject>692/4019/592/2725</subject><subject>Animal models</subject><subject>Bone marrow</subject><subject>Cardiac function</subject><subject>Cardiomyocytes</subject><subject>Cardiovascular disease</subject><subject>Enzymes</subject><subject>Glucose</subject><subject>Heart attacks</subject><subject>Hospitals</subject><subject>Humanities and Social Sciences</subject><subject>Ischemia</subject><subject>Kinases</subject><subject>Magnetic resonance imaging</subject><subject>Medical research</subject><subject>Metabolism</subject><subject>multidisciplinary</subject><subject>Myocardial infarction</subject><subject>Nuclear medicine</subject><subject>Protein expression</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Science</subject><subject>Science 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Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Min</au><au>Shen, Rui</au><au>Song, Lei</au><au>Lu, Minjie</au><au>Wang, Jianguang</au><au>Zhao, Shihua</au><au>Tang, Yue</au><au>Meng, Xianmin</au><au>Li, Zongjin</au><au>He, Zuo-Xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-06-20</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>28250</spage><epage>28250</epage><pages>28250-28250</pages><artnum>28250</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27321050</pmid><doi>10.1038/srep28250</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/100 631/532/2074 692/4019/592/2725 Animal models Bone marrow Cardiac function Cardiomyocytes Cardiovascular disease Enzymes Glucose Heart attacks Hospitals Humanities and Social Sciences Ischemia Kinases Magnetic resonance imaging Medical research Metabolism multidisciplinary Myocardial infarction Nuclear medicine Protein expression Protein synthesis Proteins Science Science (multidisciplinary) Signal transduction Stem cells Tomography Transplantation |
| title | Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects |
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