Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing
Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans . However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-gene...
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| Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.28110-28110, Article 28110 |
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| creator | Imabayashi, Yumi Moriyama, Masafumi Takeshita, Toru Ieda, Shinsuke Hayashida, Jun-Nosuke Tanaka, Akihiko Maehara, Takashi Furukawa, Sachiko Ohta, Miho Kubota, Keigo Yamauchi, Masaki Ishiguro, Noriko Yamashita, Yoshihisa Nakamura, Seiji |
| description | Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by
Candida albicans
. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other
Candida
or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-
C. albicans,
especially
C. dubliniensis
, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections. |
| doi_str_mv | 10.1038/srep28110 |
| format | Article |
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Candida albicans
. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other
Candida
or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-
C. albicans,
especially
C. dubliniensis
, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep28110</identifier><identifier>PMID: 27305838</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/23 ; 631/326/107 ; 631/326/193/2484 ; Adult ; Aging ; Antifungal agents ; Biodiversity ; Candida albicans - classification ; Candida albicans - genetics ; Candida glabrata - classification ; Candida glabrata - genetics ; Candida tropicalis - classification ; Candida tropicalis - genetics ; Candidiasis, Oral - microbiology ; Culture techniques ; DNA, Intergenic - genetics ; Drug resistance ; Female ; Health care ; High-Throughput Nucleotide Sequencing - methods ; Humanities and Social Sciences ; Humans ; Male ; Maxillofacial surgery ; Middle Aged ; Molecular Typing - methods ; multidisciplinary ; Mycological Typing Techniques - methods ; RNA, Ribosomal, 16S - genetics ; Science ; Science (multidisciplinary) ; Yeasts</subject><ispartof>Scientific reports, 2016-06, Vol.6 (1), p.28110-28110, Article 28110</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Jun 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-711c1b336c520351a4cc4e8484c4fc1b8a28ad8eec0a44c424d8f29844cc65753</citedby><cites>FETCH-LOGICAL-c438t-711c1b336c520351a4cc4e8484c4fc1b8a28ad8eec0a44c424d8f29844cc65753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910111/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910111/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27305838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imabayashi, Yumi</creatorcontrib><creatorcontrib>Moriyama, Masafumi</creatorcontrib><creatorcontrib>Takeshita, Toru</creatorcontrib><creatorcontrib>Ieda, Shinsuke</creatorcontrib><creatorcontrib>Hayashida, Jun-Nosuke</creatorcontrib><creatorcontrib>Tanaka, Akihiko</creatorcontrib><creatorcontrib>Maehara, Takashi</creatorcontrib><creatorcontrib>Furukawa, Sachiko</creatorcontrib><creatorcontrib>Ohta, Miho</creatorcontrib><creatorcontrib>Kubota, Keigo</creatorcontrib><creatorcontrib>Yamauchi, Masaki</creatorcontrib><creatorcontrib>Ishiguro, Noriko</creatorcontrib><creatorcontrib>Yamashita, Yoshihisa</creatorcontrib><creatorcontrib>Nakamura, Seiji</creatorcontrib><title>Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by
Candida albicans
. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other
Candida
or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-
C. albicans,
especially
C. dubliniensis
, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.</description><subject>38/23</subject><subject>631/326/107</subject><subject>631/326/193/2484</subject><subject>Adult</subject><subject>Aging</subject><subject>Antifungal agents</subject><subject>Biodiversity</subject><subject>Candida albicans - classification</subject><subject>Candida albicans - genetics</subject><subject>Candida glabrata - classification</subject><subject>Candida glabrata - genetics</subject><subject>Candida tropicalis - classification</subject><subject>Candida tropicalis - genetics</subject><subject>Candidiasis, Oral - microbiology</subject><subject>Culture techniques</subject><subject>DNA, Intergenic - genetics</subject><subject>Drug resistance</subject><subject>Female</subject><subject>Health care</subject><subject>High-Throughput Nucleotide Sequencing - methods</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Male</subject><subject>Maxillofacial surgery</subject><subject>Middle Aged</subject><subject>Molecular Typing - methods</subject><subject>multidisciplinary</subject><subject>Mycological Typing Techniques - methods</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Yeasts</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkc1OGzEUha2KCqKUBS9QWWJDkab138SeDRKKWkBK1U1ZW8bjGQwTe7BnaPP2vSQhSsEbX_l8Pj7yQeiEkq-UcPUtJ9czRSn5gCaMiLJgnLGDvfkIHef8QGCVrBK0OkRHTHJSKq4m6PFn7JwdO5OwCaZbZZ9xbHAzhtZ0uI89SIOPIWMfcA-jC0PGf_xwj2MCwppQ-9qbl3tj9qHFwf0ditYFl9YXcXZPowsWpE_oY2O67I63-xTd_vj-e35dLH5d3cwvF4UVXA2FpNTSO85ntmSEl9QIa4VTQgkrGlCUYcrUyjlLjIAzJmrVsErBbGelLPkUXWx8-_Fu6WoLkSGq7pNfmrTS0Xj9vxL8vW7jsxYVJZRSMDjbGqQI4fOglz5b13UmuDhmTWUllWRsJgE9fYM-xDHBT64pJRUjUgH1ZUPZFDMU1uzCUKJfWtS7FoH9vJ9-R752BsD5BsgghdalvSffuf0DxFOouw</recordid><startdate>20160616</startdate><enddate>20160616</enddate><creator>Imabayashi, Yumi</creator><creator>Moriyama, Masafumi</creator><creator>Takeshita, Toru</creator><creator>Ieda, Shinsuke</creator><creator>Hayashida, Jun-Nosuke</creator><creator>Tanaka, Akihiko</creator><creator>Maehara, Takashi</creator><creator>Furukawa, Sachiko</creator><creator>Ohta, Miho</creator><creator>Kubota, Keigo</creator><creator>Yamauchi, Masaki</creator><creator>Ishiguro, Noriko</creator><creator>Yamashita, Yoshihisa</creator><creator>Nakamura, Seiji</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160616</creationdate><title>Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing</title><author>Imabayashi, Yumi ; Moriyama, Masafumi ; Takeshita, Toru ; Ieda, Shinsuke ; Hayashida, Jun-Nosuke ; Tanaka, Akihiko ; Maehara, Takashi ; Furukawa, Sachiko ; Ohta, Miho ; Kubota, Keigo ; Yamauchi, Masaki ; Ishiguro, Noriko ; Yamashita, Yoshihisa ; Nakamura, Seiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-711c1b336c520351a4cc4e8484c4fc1b8a28ad8eec0a44c424d8f29844cc65753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>38/23</topic><topic>631/326/107</topic><topic>631/326/193/2484</topic><topic>Adult</topic><topic>Aging</topic><topic>Antifungal agents</topic><topic>Biodiversity</topic><topic>Candida albicans - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imabayashi, Yumi</au><au>Moriyama, Masafumi</au><au>Takeshita, Toru</au><au>Ieda, Shinsuke</au><au>Hayashida, Jun-Nosuke</au><au>Tanaka, Akihiko</au><au>Maehara, Takashi</au><au>Furukawa, Sachiko</au><au>Ohta, Miho</au><au>Kubota, Keigo</au><au>Yamauchi, Masaki</au><au>Ishiguro, Noriko</au><au>Yamashita, Yoshihisa</au><au>Nakamura, Seiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-06-16</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>28110</spage><epage>28110</epage><pages>28110-28110</pages><artnum>28110</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by
Candida albicans
. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other
Candida
or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-
C. albicans,
especially
C. dubliniensis
, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27305838</pmid><doi>10.1038/srep28110</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 38/23 631/326/107 631/326/193/2484 Adult Aging Antifungal agents Biodiversity Candida albicans - classification Candida albicans - genetics Candida glabrata - classification Candida glabrata - genetics Candida tropicalis - classification Candida tropicalis - genetics Candidiasis, Oral - microbiology Culture techniques DNA, Intergenic - genetics Drug resistance Female Health care High-Throughput Nucleotide Sequencing - methods Humanities and Social Sciences Humans Male Maxillofacial surgery Middle Aged Molecular Typing - methods multidisciplinary Mycological Typing Techniques - methods RNA, Ribosomal, 16S - genetics Science Science (multidisciplinary) Yeasts |
| title | Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing |
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