Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma
Interleukin-33 (IL-33), a newly-discovered cytokine belonging to the IL-1 family, serves an important role in inflammation. However, it is not clear whether IL-33 is of clinical significance in hepatocarcinogenesis. The present study was designed to investigate the role of IL-33 during oncogenesis a...
Gespeichert in:
| Veröffentlicht in: | Oncology letters 2016-07, Vol.12 (1), p.429-436 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 436 |
|---|---|
| container_issue | 1 |
| container_start_page | 429 |
| container_title | Oncology letters |
| container_volume | 12 |
| creator | YANG, YAN WANG, JUN-BIN LI, YU-MEI ZHAO, YU WANG, RUI WU, QIONG ZHENG, RONG-SHENG OU, YU-RONG |
| description | Interleukin-33 (IL-33), a newly-discovered cytokine belonging to the IL-1 family, serves an important role in inflammation. However, it is not clear whether IL-33 is of clinical significance in hepatocarcinogenesis. The present study was designed to investigate the role of IL-33 during oncogenesis and development of hepatocellular carcinoma (HCC). IL-33 protein expression was detected in 76 HCC (including 36 para-carcinoma), 33 cirrhosis, 30 hepatitis, and 20 normal liver tissues using immunohistochemistry. IL-33 mRNA expression in carcinoma and para-carcinoma tissues was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The possible correlation between IL-33 and clinicopathological parameters of HCC was also analyzed. Significant differences in IL-33 expression were not observed among normal, hepatic, and cirrhotic tissues (P>0.05), whereas the level of protein positive rate was markedly reduced in HCC tissues (P |
| doi_str_mv | 10.3892/ol.2016.4622 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4906799</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A458812106</galeid><sourcerecordid>A458812106</sourcerecordid><originalsourceid>FETCH-LOGICAL-c605t-75d92f509c4113c475e31015938115134fee49d2f0f31ea12d406852128754a13</originalsourceid><addsrcrecordid>eNptks9rFDEUxwdRbKm9eZYBRXpw1rz8nLkUSqlaWCiIHjyFmHmzm5JJxslM0f_eTLeuXTHvkJB83jd5L9-ieAlkxeqGvo9-RQnIFZeUPimOQTW0AlLTp_u14kfFaUq3JA8hoa7l8-KIKsYVSHpcfPscPZaxK6_XFWMl_hxGTMnFULpQxmDjBgMml0oT2rLFO_Rx6DFMS8p27k0otziYKVr0fvZmLK0ZrQuxNy-KZ53xCU8f5pPi64erL5efqvXNx-vLi3VlJRFTpUTb0E6QxnIAZrkSyICAaFgNIIDxDpE3Le1IxwAN0JYTWQsKtFaCG2AnxflOd5i_99ja_LjReD2MrjfjLx2N04cnwW31Jt5p3hCpmiYLnD0IjPHHjGnSvUtLPSZgnJOGmkoFTAma0df_oLdxHkMuT0PD7jGAv9TGeNQudDHfaxdRfcFFXQMFIjO1-g-Vo8Xe2Riwc3n_IOHto4QtGj9tU_TzlH8rHYLvdqAdY0ojdvtmANGLbXT0erGNXmyT8VePG7iH_5gkA292QBqyC1wb0565WVckx73Ob7b8xTg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932671311</pqid></control><display><type>article</type><title>Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma</title><source>Spandidos Publications Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>YANG, YAN ; WANG, JUN-BIN ; LI, YU-MEI ; ZHAO, YU ; WANG, RUI ; WU, QIONG ; ZHENG, RONG-SHENG ; OU, YU-RONG</creator><creatorcontrib>YANG, YAN ; WANG, JUN-BIN ; LI, YU-MEI ; ZHAO, YU ; WANG, RUI ; WU, QIONG ; ZHENG, RONG-SHENG ; OU, YU-RONG</creatorcontrib><description>Interleukin-33 (IL-33), a newly-discovered cytokine belonging to the IL-1 family, serves an important role in inflammation. However, it is not clear whether IL-33 is of clinical significance in hepatocarcinogenesis. The present study was designed to investigate the role of IL-33 during oncogenesis and development of hepatocellular carcinoma (HCC). IL-33 protein expression was detected in 76 HCC (including 36 para-carcinoma), 33 cirrhosis, 30 hepatitis, and 20 normal liver tissues using immunohistochemistry. IL-33 mRNA expression in carcinoma and para-carcinoma tissues was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The possible correlation between IL-33 and clinicopathological parameters of HCC was also analyzed. Significant differences in IL-33 expression were not observed among normal, hepatic, and cirrhotic tissues (P>0.05), whereas the level of protein positive rate was markedly reduced in HCC tissues (P<0.01). Positive staining of IL-33 in non-cancerous liver (NCL) tissues (i.e. normal, hepatitis, and liver cirrhosis) was located predominantly in the nucleus and occasionally in the cytoplasm of hepatocytes; however, the expression in HCC tissues was mostly restricted to the cytoplasm. A significant alteration in protein localization was observed in HCC tissues as compared with NCL tissues (P<0.01). In comparison with HCC tissues, cytoplasmic staining of IL-33 was increased in para-carcinoma tissues. RT-PCR assay further confirmed relatively high mRNA expression levels of IL-33 in para-carcinoma tissues. IL-33 expression was significantly negatively associated with tumor histological grade (r=−0.279, P=0.015), but not with year, gender, tumor size, clinical stage, HCC with hepatitis and cirrhosis background, lymph node metastasis or intrahepatic vascular embolism (P>0.05). Therefore, the aberrant expression of IL-33 is associated with oncogenesis and progression of HCC and the cytoplasmic accumulation of the protein may serve a role in hepatocarcinogenesis.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2016.4622</identifier><identifier>PMID: 27347162</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Age ; Biotechnology ; Breast cancer ; Cytokines ; Development and progression ; Gene expression ; Genetic aspects ; Health aspects ; Hepatitis ; hepatocarcinogenesis ; hepatocellular carcinoma ; Hepatoma ; immunohistochemistry ; inflammation ; interleukin-33 ; Interleukins ; Kinases ; Liver cancer ; Liver cirrhosis ; Liver diseases ; Localization ; Males ; Oncology ; Pathogenesis ; Patients ; Proteins ; Studies ; Tumors</subject><ispartof>Oncology letters, 2016-07, Vol.12 (1), p.429-436</ispartof><rights>Copyright: © Yang et al.</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><rights>Copyright: © Yang et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c605t-75d92f509c4113c475e31015938115134fee49d2f0f31ea12d406852128754a13</citedby><cites>FETCH-LOGICAL-c605t-75d92f509c4113c475e31015938115134fee49d2f0f31ea12d406852128754a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906799/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906799/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,5571,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27347162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YANG, YAN</creatorcontrib><creatorcontrib>WANG, JUN-BIN</creatorcontrib><creatorcontrib>LI, YU-MEI</creatorcontrib><creatorcontrib>ZHAO, YU</creatorcontrib><creatorcontrib>WANG, RUI</creatorcontrib><creatorcontrib>WU, QIONG</creatorcontrib><creatorcontrib>ZHENG, RONG-SHENG</creatorcontrib><creatorcontrib>OU, YU-RONG</creatorcontrib><title>Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Interleukin-33 (IL-33), a newly-discovered cytokine belonging to the IL-1 family, serves an important role in inflammation. However, it is not clear whether IL-33 is of clinical significance in hepatocarcinogenesis. The present study was designed to investigate the role of IL-33 during oncogenesis and development of hepatocellular carcinoma (HCC). IL-33 protein expression was detected in 76 HCC (including 36 para-carcinoma), 33 cirrhosis, 30 hepatitis, and 20 normal liver tissues using immunohistochemistry. IL-33 mRNA expression in carcinoma and para-carcinoma tissues was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The possible correlation between IL-33 and clinicopathological parameters of HCC was also analyzed. Significant differences in IL-33 expression were not observed among normal, hepatic, and cirrhotic tissues (P>0.05), whereas the level of protein positive rate was markedly reduced in HCC tissues (P<0.01). Positive staining of IL-33 in non-cancerous liver (NCL) tissues (i.e. normal, hepatitis, and liver cirrhosis) was located predominantly in the nucleus and occasionally in the cytoplasm of hepatocytes; however, the expression in HCC tissues was mostly restricted to the cytoplasm. A significant alteration in protein localization was observed in HCC tissues as compared with NCL tissues (P<0.01). In comparison with HCC tissues, cytoplasmic staining of IL-33 was increased in para-carcinoma tissues. RT-PCR assay further confirmed relatively high mRNA expression levels of IL-33 in para-carcinoma tissues. IL-33 expression was significantly negatively associated with tumor histological grade (r=−0.279, P=0.015), but not with year, gender, tumor size, clinical stage, HCC with hepatitis and cirrhosis background, lymph node metastasis or intrahepatic vascular embolism (P>0.05). Therefore, the aberrant expression of IL-33 is associated with oncogenesis and progression of HCC and the cytoplasmic accumulation of the protein may serve a role in hepatocarcinogenesis.</description><subject>Age</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cytokines</subject><subject>Development and progression</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hepatitis</subject><subject>hepatocarcinogenesis</subject><subject>hepatocellular carcinoma</subject><subject>Hepatoma</subject><subject>immunohistochemistry</subject><subject>inflammation</subject><subject>interleukin-33</subject><subject>Interleukins</subject><subject>Kinases</subject><subject>Liver cancer</subject><subject>Liver cirrhosis</subject><subject>Liver diseases</subject><subject>Localization</subject><subject>Males</subject><subject>Oncology</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Proteins</subject><subject>Studies</subject><subject>Tumors</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks9rFDEUxwdRbKm9eZYBRXpw1rz8nLkUSqlaWCiIHjyFmHmzm5JJxslM0f_eTLeuXTHvkJB83jd5L9-ieAlkxeqGvo9-RQnIFZeUPimOQTW0AlLTp_u14kfFaUq3JA8hoa7l8-KIKsYVSHpcfPscPZaxK6_XFWMl_hxGTMnFULpQxmDjBgMml0oT2rLFO_Rx6DFMS8p27k0otziYKVr0fvZmLK0ZrQuxNy-KZ53xCU8f5pPi64erL5efqvXNx-vLi3VlJRFTpUTb0E6QxnIAZrkSyICAaFgNIIDxDpE3Le1IxwAN0JYTWQsKtFaCG2AnxflOd5i_99ja_LjReD2MrjfjLx2N04cnwW31Jt5p3hCpmiYLnD0IjPHHjGnSvUtLPSZgnJOGmkoFTAma0df_oLdxHkMuT0PD7jGAv9TGeNQudDHfaxdRfcFFXQMFIjO1-g-Vo8Xe2Riwc3n_IOHto4QtGj9tU_TzlH8rHYLvdqAdY0ojdvtmANGLbXT0erGNXmyT8VePG7iH_5gkA292QBqyC1wb0565WVckx73Ob7b8xTg</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>YANG, YAN</creator><creator>WANG, JUN-BIN</creator><creator>LI, YU-MEI</creator><creator>ZHAO, YU</creator><creator>WANG, RUI</creator><creator>WU, QIONG</creator><creator>ZHENG, RONG-SHENG</creator><creator>OU, YU-RONG</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160701</creationdate><title>Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma</title><author>YANG, YAN ; WANG, JUN-BIN ; LI, YU-MEI ; ZHAO, YU ; WANG, RUI ; WU, QIONG ; ZHENG, RONG-SHENG ; OU, YU-RONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c605t-75d92f509c4113c475e31015938115134fee49d2f0f31ea12d406852128754a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Age</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cytokines</topic><topic>Development and progression</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hepatitis</topic><topic>hepatocarcinogenesis</topic><topic>hepatocellular carcinoma</topic><topic>Hepatoma</topic><topic>immunohistochemistry</topic><topic>inflammation</topic><topic>interleukin-33</topic><topic>Interleukins</topic><topic>Kinases</topic><topic>Liver cancer</topic><topic>Liver cirrhosis</topic><topic>Liver diseases</topic><topic>Localization</topic><topic>Males</topic><topic>Oncology</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Proteins</topic><topic>Studies</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YANG, YAN</creatorcontrib><creatorcontrib>WANG, JUN-BIN</creatorcontrib><creatorcontrib>LI, YU-MEI</creatorcontrib><creatorcontrib>ZHAO, YU</creatorcontrib><creatorcontrib>WANG, RUI</creatorcontrib><creatorcontrib>WU, QIONG</creatorcontrib><creatorcontrib>ZHENG, RONG-SHENG</creatorcontrib><creatorcontrib>OU, YU-RONG</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest - Health & Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YANG, YAN</au><au>WANG, JUN-BIN</au><au>LI, YU-MEI</au><au>ZHAO, YU</au><au>WANG, RUI</au><au>WU, QIONG</au><au>ZHENG, RONG-SHENG</au><au>OU, YU-RONG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>12</volume><issue>1</issue><spage>429</spage><epage>436</epage><pages>429-436</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>Interleukin-33 (IL-33), a newly-discovered cytokine belonging to the IL-1 family, serves an important role in inflammation. However, it is not clear whether IL-33 is of clinical significance in hepatocarcinogenesis. The present study was designed to investigate the role of IL-33 during oncogenesis and development of hepatocellular carcinoma (HCC). IL-33 protein expression was detected in 76 HCC (including 36 para-carcinoma), 33 cirrhosis, 30 hepatitis, and 20 normal liver tissues using immunohistochemistry. IL-33 mRNA expression in carcinoma and para-carcinoma tissues was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The possible correlation between IL-33 and clinicopathological parameters of HCC was also analyzed. Significant differences in IL-33 expression were not observed among normal, hepatic, and cirrhotic tissues (P>0.05), whereas the level of protein positive rate was markedly reduced in HCC tissues (P<0.01). Positive staining of IL-33 in non-cancerous liver (NCL) tissues (i.e. normal, hepatitis, and liver cirrhosis) was located predominantly in the nucleus and occasionally in the cytoplasm of hepatocytes; however, the expression in HCC tissues was mostly restricted to the cytoplasm. A significant alteration in protein localization was observed in HCC tissues as compared with NCL tissues (P<0.01). In comparison with HCC tissues, cytoplasmic staining of IL-33 was increased in para-carcinoma tissues. RT-PCR assay further confirmed relatively high mRNA expression levels of IL-33 in para-carcinoma tissues. IL-33 expression was significantly negatively associated with tumor histological grade (r=−0.279, P=0.015), but not with year, gender, tumor size, clinical stage, HCC with hepatitis and cirrhosis background, lymph node metastasis or intrahepatic vascular embolism (P>0.05). Therefore, the aberrant expression of IL-33 is associated with oncogenesis and progression of HCC and the cytoplasmic accumulation of the protein may serve a role in hepatocarcinogenesis.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27347162</pmid><doi>10.3892/ol.2016.4622</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2016-07, Vol.12 (1), p.429-436 |
| issn | 1792-1074 1792-1082 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4906799 |
| source | Spandidos Publications Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Age Biotechnology Breast cancer Cytokines Development and progression Gene expression Genetic aspects Health aspects Hepatitis hepatocarcinogenesis hepatocellular carcinoma Hepatoma immunohistochemistry inflammation interleukin-33 Interleukins Kinases Liver cancer Liver cirrhosis Liver diseases Localization Males Oncology Pathogenesis Patients Proteins Studies Tumors |
| title | Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T16%3A17%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20IL-33%20expression%20in%20oncogenesis%20and%20development%20of%20human%20hepatocellular%20carcinoma&rft.jtitle=Oncology%20letters&rft.au=YANG,%20YAN&rft.date=2016-07-01&rft.volume=12&rft.issue=1&rft.spage=429&rft.epage=436&rft.pages=429-436&rft.issn=1792-1074&rft.eissn=1792-1082&rft_id=info:doi/10.3892/ol.2016.4622&rft_dat=%3Cgale_pubme%3EA458812106%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932671311&rft_id=info:pmid/27347162&rft_galeid=A458812106&rfr_iscdi=true |