MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis

Osteoarthritis (OA) is the most prevalent degenerative joint disease with multifactorial etiology caused by risk factors such as ageing, obesity and trauma. Previously, it was reported that the inhibition of microRNA-34a (miR-34a) may reduce rat chondrocyte apoptosis induced by IL-1β, whereas the mo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of molecular medicine 2016-07, Vol.38 (1), p.201-209
Hauptverfasser: YAN, SHIJU, WANG, MENG, ZHAO, JIAN, ZHANG, HONGTAO, ZHOU, CHENGPEI, JIN, LEI, ZHANG, YINGLONG, QIU, XIUCHUN, MA, BAOAN, FAN, QINGYU
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 209
container_issue 1
container_start_page 201
container_title International journal of molecular medicine
container_volume 38
creator YAN, SHIJU
WANG, MENG
ZHAO, JIAN
ZHANG, HONGTAO
ZHOU, CHENGPEI
JIN, LEI
ZHANG, YINGLONG
QIU, XIUCHUN
MA, BAOAN
FAN, QINGYU
description Osteoarthritis (OA) is the most prevalent degenerative joint disease with multifactorial etiology caused by risk factors such as ageing, obesity and trauma. Previously, it was reported that the inhibition of microRNA-34a (miR-34a) may reduce rat chondrocyte apoptosis induced by IL-1β, whereas the molecular mechanism and the role of miR-34a in human chondrocyte as well as in OA progression remains to be determined. In the current study, using MTT, luciferase reporter assays and western blot analysis we identified that miR-34a was upregulated while silent information regulator 1 (SIRT1) was inhibited in chondrocytes from 12 OA patients compared with healthy chondrocytes from 10 trauma amputees. Overexpression of miR-34a promoted apoptosis and inhibited cell proliferation in human chondrocytes. Transfection with miR-34a mimic inhibited SIRT1 expression, which attenuated the deacetylation of p53, leading to the upregulation of Bax and downregulation of Bcl-2. Furthermore, results from the western blot analysis and luciferase reporter assay demonstrated that SIRT1 was directly regulated by miR-34a in human chondrocytes. A rat model of OA was induced through anterior cruciate ligament transection and medial meniscus resection (ACLT+MMx). The results showed that the intra-articular injection of lentiviral vector encoding anti-miR-34a sequence effectively ameliorated the progression of OA. The results suggest that miR-34a has a crucial role in the pathogenesis of OA through direct regulation of the SIRT1/p53 signaling pathway and serves as a potential therapeutic target of OA.
doi_str_mv 10.3892/ijmm.2016.2618
format Article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4899008</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A457330627</galeid><sourcerecordid>A457330627</sourcerecordid><originalsourceid>FETCH-LOGICAL-c584t-2ec20ef75d15c4d5196e2d559930407fa058927c46cbf6ba8215f740c3a605c43</originalsourceid><addsrcrecordid>eNptks1rFDEYxgex2Fq9epSAFy-zzedkchGW4kehttBW8BaymWQmy0wyJlll_w7_YTO0XRVKDglPfu9D3rxPVb1BcEVagc_cdppWGKJmhRvUPqtOEBeoxpR-f17OCPKacNYcVy9T2kKIGRXti-oYc0w5xu1J9fur0zHcXK1rQhVQ1hqdE9BD8F0Mep8NUHOYc0guAeU7MMcwOmuiyi54sNmDrGJvsvM9yIMBtxc3d-hsZgQk13s1Lvqs8vBL7UG3i4_YIoXeeLPYBgtCyiaomIfoskuvqiOrxmReP-yn1bdPH-_Ov9SX158vzteXtWYtzTU2GkNjOesQ07RjSDQGd4wJQSCF3CrIyg9xTRu9sc1GtRgxyynURDWwVJDT6sO977zbTKbTxueoRjlHN6m4l0E5-f-Nd4Psw09JWyEgbIvBuweDGH7sTMpyG3axtJ0kEgQTLARnf6lejUY6b0Mx05NLWq4p44TABvNCrZ6gyurM5HTwxrqiP1VQ5pdSNPbwcATlkg25ZEMu2ZBLNkrB23_bPeCPYSjA-3sgzWXUrgvpwCxWNWlriGpYHMkfTUjEig</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932329975</pqid></control><display><type>article</type><title>MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>YAN, SHIJU ; WANG, MENG ; ZHAO, JIAN ; ZHANG, HONGTAO ; ZHOU, CHENGPEI ; JIN, LEI ; ZHANG, YINGLONG ; QIU, XIUCHUN ; MA, BAOAN ; FAN, QINGYU</creator><creatorcontrib>YAN, SHIJU ; WANG, MENG ; ZHAO, JIAN ; ZHANG, HONGTAO ; ZHOU, CHENGPEI ; JIN, LEI ; ZHANG, YINGLONG ; QIU, XIUCHUN ; MA, BAOAN ; FAN, QINGYU</creatorcontrib><description>Osteoarthritis (OA) is the most prevalent degenerative joint disease with multifactorial etiology caused by risk factors such as ageing, obesity and trauma. Previously, it was reported that the inhibition of microRNA-34a (miR-34a) may reduce rat chondrocyte apoptosis induced by IL-1β, whereas the molecular mechanism and the role of miR-34a in human chondrocyte as well as in OA progression remains to be determined. In the current study, using MTT, luciferase reporter assays and western blot analysis we identified that miR-34a was upregulated while silent information regulator 1 (SIRT1) was inhibited in chondrocytes from 12 OA patients compared with healthy chondrocytes from 10 trauma amputees. Overexpression of miR-34a promoted apoptosis and inhibited cell proliferation in human chondrocytes. Transfection with miR-34a mimic inhibited SIRT1 expression, which attenuated the deacetylation of p53, leading to the upregulation of Bax and downregulation of Bcl-2. Furthermore, results from the western blot analysis and luciferase reporter assay demonstrated that SIRT1 was directly regulated by miR-34a in human chondrocytes. A rat model of OA was induced through anterior cruciate ligament transection and medial meniscus resection (ACLT+MMx). The results showed that the intra-articular injection of lentiviral vector encoding anti-miR-34a sequence effectively ameliorated the progression of OA. The results suggest that miR-34a has a crucial role in the pathogenesis of OA through direct regulation of the SIRT1/p53 signaling pathway and serves as a potential therapeutic target of OA.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2016.2618</identifier><identifier>PMID: 27247228</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Animals ; Apoptosis ; Apoptosis - genetics ; Arthritis ; Binding sites ; Care and treatment ; Cartilage - metabolism ; Cartilage - pathology ; Case-Control Studies ; Cell cycle ; Cell growth ; Cell Proliferation ; Cellular signal transduction ; chondrocyte ; Chondrocytes - metabolism ; Chondrocytes - pathology ; Colorectal cancer ; Development and progression ; Disease ; Disease Progression ; Gene expression ; Genetic aspects ; Health aspects ; Humans ; Inflammation ; Joint surgery ; lentivirus ; Male ; MicroRNA ; microRNA-34a ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; Older people ; Oligonucleotides - metabolism ; Osteoarthritis ; Osteoarthritis - genetics ; Osteoarthritis - pathology ; p53 ; Pathogenesis ; Physiology ; Properties ; Rats, Sprague-Dawley ; Reproducibility of Results ; Senescence ; Signal Transduction ; silent information regulator 1 ; Sirtuin 1 - metabolism ; Studies ; Surgery ; Transfection ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>International journal of molecular medicine, 2016-07, Vol.38 (1), p.201-209</ispartof><rights>Copyright: © Yan et al.</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><rights>Copyright: © Yan et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-2ec20ef75d15c4d5196e2d559930407fa058927c46cbf6ba8215f740c3a605c43</citedby><cites>FETCH-LOGICAL-c584t-2ec20ef75d15c4d5196e2d559930407fa058927c46cbf6ba8215f740c3a605c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27247228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAN, SHIJU</creatorcontrib><creatorcontrib>WANG, MENG</creatorcontrib><creatorcontrib>ZHAO, JIAN</creatorcontrib><creatorcontrib>ZHANG, HONGTAO</creatorcontrib><creatorcontrib>ZHOU, CHENGPEI</creatorcontrib><creatorcontrib>JIN, LEI</creatorcontrib><creatorcontrib>ZHANG, YINGLONG</creatorcontrib><creatorcontrib>QIU, XIUCHUN</creatorcontrib><creatorcontrib>MA, BAOAN</creatorcontrib><creatorcontrib>FAN, QINGYU</creatorcontrib><title>MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Osteoarthritis (OA) is the most prevalent degenerative joint disease with multifactorial etiology caused by risk factors such as ageing, obesity and trauma. Previously, it was reported that the inhibition of microRNA-34a (miR-34a) may reduce rat chondrocyte apoptosis induced by IL-1β, whereas the molecular mechanism and the role of miR-34a in human chondrocyte as well as in OA progression remains to be determined. In the current study, using MTT, luciferase reporter assays and western blot analysis we identified that miR-34a was upregulated while silent information regulator 1 (SIRT1) was inhibited in chondrocytes from 12 OA patients compared with healthy chondrocytes from 10 trauma amputees. Overexpression of miR-34a promoted apoptosis and inhibited cell proliferation in human chondrocytes. Transfection with miR-34a mimic inhibited SIRT1 expression, which attenuated the deacetylation of p53, leading to the upregulation of Bax and downregulation of Bcl-2. Furthermore, results from the western blot analysis and luciferase reporter assay demonstrated that SIRT1 was directly regulated by miR-34a in human chondrocytes. A rat model of OA was induced through anterior cruciate ligament transection and medial meniscus resection (ACLT+MMx). The results showed that the intra-articular injection of lentiviral vector encoding anti-miR-34a sequence effectively ameliorated the progression of OA. The results suggest that miR-34a has a crucial role in the pathogenesis of OA through direct regulation of the SIRT1/p53 signaling pathway and serves as a potential therapeutic target of OA.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Arthritis</subject><subject>Binding sites</subject><subject>Care and treatment</subject><subject>Cartilage - metabolism</subject><subject>Cartilage - pathology</subject><subject>Case-Control Studies</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cellular signal transduction</subject><subject>chondrocyte</subject><subject>Chondrocytes - metabolism</subject><subject>Chondrocytes - pathology</subject><subject>Colorectal cancer</subject><subject>Development and progression</subject><subject>Disease</subject><subject>Disease Progression</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Joint surgery</subject><subject>lentivirus</subject><subject>Male</subject><subject>MicroRNA</subject><subject>microRNA-34a</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>Older people</subject><subject>Oligonucleotides - metabolism</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - genetics</subject><subject>Osteoarthritis - pathology</subject><subject>p53</subject><subject>Pathogenesis</subject><subject>Physiology</subject><subject>Properties</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>Senescence</subject><subject>Signal Transduction</subject><subject>silent information regulator 1</subject><subject>Sirtuin 1 - metabolism</subject><subject>Studies</subject><subject>Surgery</subject><subject>Transfection</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks1rFDEYxgex2Fq9epSAFy-zzedkchGW4kehttBW8BaymWQmy0wyJlll_w7_YTO0XRVKDglPfu9D3rxPVb1BcEVagc_cdppWGKJmhRvUPqtOEBeoxpR-f17OCPKacNYcVy9T2kKIGRXti-oYc0w5xu1J9fur0zHcXK1rQhVQ1hqdE9BD8F0Mep8NUHOYc0guAeU7MMcwOmuiyi54sNmDrGJvsvM9yIMBtxc3d-hsZgQk13s1Lvqs8vBL7UG3i4_YIoXeeLPYBgtCyiaomIfoskuvqiOrxmReP-yn1bdPH-_Ov9SX158vzteXtWYtzTU2GkNjOesQ07RjSDQGd4wJQSCF3CrIyg9xTRu9sc1GtRgxyynURDWwVJDT6sO977zbTKbTxueoRjlHN6m4l0E5-f-Nd4Psw09JWyEgbIvBuweDGH7sTMpyG3axtJ0kEgQTLARnf6lejUY6b0Mx05NLWq4p44TABvNCrZ6gyurM5HTwxrqiP1VQ5pdSNPbwcATlkg25ZEMu2ZBLNkrB23_bPeCPYSjA-3sgzWXUrgvpwCxWNWlriGpYHMkfTUjEig</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>YAN, SHIJU</creator><creator>WANG, MENG</creator><creator>ZHAO, JIAN</creator><creator>ZHANG, HONGTAO</creator><creator>ZHOU, CHENGPEI</creator><creator>JIN, LEI</creator><creator>ZHANG, YINGLONG</creator><creator>QIU, XIUCHUN</creator><creator>MA, BAOAN</creator><creator>FAN, QINGYU</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20160701</creationdate><title>MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis</title><author>YAN, SHIJU ; WANG, MENG ; ZHAO, JIAN ; ZHANG, HONGTAO ; ZHOU, CHENGPEI ; JIN, LEI ; ZHANG, YINGLONG ; QIU, XIUCHUN ; MA, BAOAN ; FAN, QINGYU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-2ec20ef75d15c4d5196e2d559930407fa058927c46cbf6ba8215f740c3a605c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - genetics</topic><topic>Arthritis</topic><topic>Binding sites</topic><topic>Care and treatment</topic><topic>Cartilage - metabolism</topic><topic>Cartilage - pathology</topic><topic>Case-Control Studies</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Cellular signal transduction</topic><topic>chondrocyte</topic><topic>Chondrocytes - metabolism</topic><topic>Chondrocytes - pathology</topic><topic>Colorectal cancer</topic><topic>Development and progression</topic><topic>Disease</topic><topic>Disease Progression</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Joint surgery</topic><topic>lentivirus</topic><topic>Male</topic><topic>MicroRNA</topic><topic>microRNA-34a</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>Older people</topic><topic>Oligonucleotides - metabolism</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis - genetics</topic><topic>Osteoarthritis - pathology</topic><topic>p53</topic><topic>Pathogenesis</topic><topic>Physiology</topic><topic>Properties</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>Senescence</topic><topic>Signal Transduction</topic><topic>silent information regulator 1</topic><topic>Sirtuin 1 - metabolism</topic><topic>Studies</topic><topic>Surgery</topic><topic>Transfection</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAN, SHIJU</creatorcontrib><creatorcontrib>WANG, MENG</creatorcontrib><creatorcontrib>ZHAO, JIAN</creatorcontrib><creatorcontrib>ZHANG, HONGTAO</creatorcontrib><creatorcontrib>ZHOU, CHENGPEI</creatorcontrib><creatorcontrib>JIN, LEI</creatorcontrib><creatorcontrib>ZHANG, YINGLONG</creatorcontrib><creatorcontrib>QIU, XIUCHUN</creatorcontrib><creatorcontrib>MA, BAOAN</creatorcontrib><creatorcontrib>FAN, QINGYU</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAN, SHIJU</au><au>WANG, MENG</au><au>ZHAO, JIAN</au><au>ZHANG, HONGTAO</au><au>ZHOU, CHENGPEI</au><au>JIN, LEI</au><au>ZHANG, YINGLONG</au><au>QIU, XIUCHUN</au><au>MA, BAOAN</au><au>FAN, QINGYU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>38</volume><issue>1</issue><spage>201</spage><epage>209</epage><pages>201-209</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Osteoarthritis (OA) is the most prevalent degenerative joint disease with multifactorial etiology caused by risk factors such as ageing, obesity and trauma. Previously, it was reported that the inhibition of microRNA-34a (miR-34a) may reduce rat chondrocyte apoptosis induced by IL-1β, whereas the molecular mechanism and the role of miR-34a in human chondrocyte as well as in OA progression remains to be determined. In the current study, using MTT, luciferase reporter assays and western blot analysis we identified that miR-34a was upregulated while silent information regulator 1 (SIRT1) was inhibited in chondrocytes from 12 OA patients compared with healthy chondrocytes from 10 trauma amputees. Overexpression of miR-34a promoted apoptosis and inhibited cell proliferation in human chondrocytes. Transfection with miR-34a mimic inhibited SIRT1 expression, which attenuated the deacetylation of p53, leading to the upregulation of Bax and downregulation of Bcl-2. Furthermore, results from the western blot analysis and luciferase reporter assay demonstrated that SIRT1 was directly regulated by miR-34a in human chondrocytes. A rat model of OA was induced through anterior cruciate ligament transection and medial meniscus resection (ACLT+MMx). The results showed that the intra-articular injection of lentiviral vector encoding anti-miR-34a sequence effectively ameliorated the progression of OA. The results suggest that miR-34a has a crucial role in the pathogenesis of OA through direct regulation of the SIRT1/p53 signaling pathway and serves as a potential therapeutic target of OA.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27247228</pmid><doi>10.3892/ijmm.2016.2618</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1107-3756
ispartof International journal of molecular medicine, 2016-07, Vol.38 (1), p.201-209
issn 1107-3756
1791-244X
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4899008
source Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Animals
Apoptosis
Apoptosis - genetics
Arthritis
Binding sites
Care and treatment
Cartilage - metabolism
Cartilage - pathology
Case-Control Studies
Cell cycle
Cell growth
Cell Proliferation
Cellular signal transduction
chondrocyte
Chondrocytes - metabolism
Chondrocytes - pathology
Colorectal cancer
Development and progression
Disease
Disease Progression
Gene expression
Genetic aspects
Health aspects
Humans
Inflammation
Joint surgery
lentivirus
Male
MicroRNA
microRNA-34a
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Older people
Oligonucleotides - metabolism
Osteoarthritis
Osteoarthritis - genetics
Osteoarthritis - pathology
p53
Pathogenesis
Physiology
Properties
Rats, Sprague-Dawley
Reproducibility of Results
Senescence
Signal Transduction
silent information regulator 1
Sirtuin 1 - metabolism
Studies
Surgery
Transfection
Tumor Suppressor Protein p53 - metabolism
title MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A24%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNA-34a%20affects%20chondrocyte%20apoptosis%20and%20proliferation%20by%20targeting%20the%20SIRT1/p53%20signaling%20pathway%20during%20the%20pathogenesis%20of%20osteoarthritis&rft.jtitle=International%20journal%20of%20molecular%20medicine&rft.au=YAN,%20SHIJU&rft.date=2016-07-01&rft.volume=38&rft.issue=1&rft.spage=201&rft.epage=209&rft.pages=201-209&rft.issn=1107-3756&rft.eissn=1791-244X&rft_id=info:doi/10.3892/ijmm.2016.2618&rft_dat=%3Cgale_pubme%3EA457330627%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932329975&rft_id=info:pmid/27247228&rft_galeid=A457330627&rfr_iscdi=true