Programmable DNA Nanosystem for Molecular Interrogation
We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whos...
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| Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.27413-27413, Article 27413 |
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| creator | Mathur, Divita Henderson, Eric R. |
| description | We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic. |
| doi_str_mv | 10.1038/srep27413 |
| format | Article |
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| subjects | 14/63 631/61/350/2093 639/925/926 639/925/926/1048 Deoxyribonucleic acid DNA DNA - chemistry Fluorescence Resonance Energy Transfer Humanities and Social Sciences Hybridization Microscopy, Electron, Transmission multidisciplinary Nanostructures Nucleic acids Science |
| title | Programmable DNA Nanosystem for Molecular Interrogation |
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