Inhibition of Osteoclast Differentiation and Bone Resorption by Bisphosphonate-conjugated Gold Nanoparticles
In recent years, gold nanoparticles (GNPs) have been reported to affect the regeneration of bone tissue. The goal of this study was to improve bone tissue regeneration by using targeted GNPs. We fabricated a functionalized GNPs conjugated with alendronate (ALD), of the bisphosphonate group. Subseque...
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Veröffentlicht in: | Scientific reports 2016-06, Vol.6 (1), p.27336-27336, Article 27336 |
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creator | Lee, Donghyun Heo, Dong Nyoung Kim, Han-Jun Ko, Wan-Kyu Lee, Sang Jin Heo, Min Bang, Jae Beum Lee, Jung Bok Hwang, Deok-Sang Do, Sun Hee Kwon, Il Keun |
description | In recent years, gold nanoparticles (GNPs) have been reported to affect the regeneration of bone tissue. The goal of this study was to improve bone tissue regeneration by using targeted GNPs. We fabricated a functionalized GNPs conjugated with alendronate (ALD), of the bisphosphonate group. Subsequently, the ALD, GNPs, and ALD conjugated GNPs (GNPs-ALD) were analyzed by ultraviolet-visible absorbance (UV-vis) spectrophotometer, Attenuated total reflectance Fourier transform infrared spectrometer (ATR-FTIR), and thermo gravimetric analysis (TGA). The prepared GNPs-ALD were used to investigate their inhibitory effects on the receptor activator of nuclear factor- κb ligand (RANKL)-induced osteoclastogenesis in bone marrow-derived macrophages (BMMs). Additionally, the GNPs-ALD were applied to ovariectomy (OVX)-induced osteoporotic mice and the experiments were evaluated. ALD was found to be successfully conjugated to the GNPs surface, and it displayed significant adhesion onto the bone surface. The
in-vitro
study indicated that the GNPs, ALD and GNPs-ALD suppressed osteoclast formation in a dose-dependent manner. Furthermore, in the OVX mouse model, the mice treated GNPs-ALD had higher bone density as compared to other OVX mice groups. The results from these tests indicated that GNPs-ALD can be useful agents for preventing and treating osteoporosis. |
doi_str_mv | 10.1038/srep27336 |
format | Article |
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in-vitro
study indicated that the GNPs, ALD and GNPs-ALD suppressed osteoclast formation in a dose-dependent manner. Furthermore, in the OVX mouse model, the mice treated GNPs-ALD had higher bone density as compared to other OVX mice groups. The results from these tests indicated that GNPs-ALD can be useful agents for preventing and treating osteoporosis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep27336</identifier><identifier>PMID: 27251863</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 14/63 ; 631/1647/350/354 ; 692/699/1670/316/801 ; Humanities and Social Sciences ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2016-06, Vol.6 (1), p.27336-27336, Article 27336</ispartof><rights>The Author(s) 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-babcca4bb30c7dadacf49141d9220ce84028b22b2c0d18ecddaa2be9f01992633</citedby><cites>FETCH-LOGICAL-c410t-babcca4bb30c7dadacf49141d9220ce84028b22b2c0d18ecddaa2be9f01992633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890291/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890291/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27251863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Donghyun</creatorcontrib><creatorcontrib>Heo, Dong Nyoung</creatorcontrib><creatorcontrib>Kim, Han-Jun</creatorcontrib><creatorcontrib>Ko, Wan-Kyu</creatorcontrib><creatorcontrib>Lee, Sang Jin</creatorcontrib><creatorcontrib>Heo, Min</creatorcontrib><creatorcontrib>Bang, Jae Beum</creatorcontrib><creatorcontrib>Lee, Jung Bok</creatorcontrib><creatorcontrib>Hwang, Deok-Sang</creatorcontrib><creatorcontrib>Do, Sun Hee</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><title>Inhibition of Osteoclast Differentiation and Bone Resorption by Bisphosphonate-conjugated Gold Nanoparticles</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In recent years, gold nanoparticles (GNPs) have been reported to affect the regeneration of bone tissue. The goal of this study was to improve bone tissue regeneration by using targeted GNPs. We fabricated a functionalized GNPs conjugated with alendronate (ALD), of the bisphosphonate group. Subsequently, the ALD, GNPs, and ALD conjugated GNPs (GNPs-ALD) were analyzed by ultraviolet-visible absorbance (UV-vis) spectrophotometer, Attenuated total reflectance Fourier transform infrared spectrometer (ATR-FTIR), and thermo gravimetric analysis (TGA). The prepared GNPs-ALD were used to investigate their inhibitory effects on the receptor activator of nuclear factor- κb ligand (RANKL)-induced osteoclastogenesis in bone marrow-derived macrophages (BMMs). Additionally, the GNPs-ALD were applied to ovariectomy (OVX)-induced osteoporotic mice and the experiments were evaluated. ALD was found to be successfully conjugated to the GNPs surface, and it displayed significant adhesion onto the bone surface. The
in-vitro
study indicated that the GNPs, ALD and GNPs-ALD suppressed osteoclast formation in a dose-dependent manner. Furthermore, in the OVX mouse model, the mice treated GNPs-ALD had higher bone density as compared to other OVX mice groups. The results from these tests indicated that GNPs-ALD can be useful agents for preventing and treating osteoporosis.</description><subject>101/28</subject><subject>14/63</subject><subject>631/1647/350/354</subject><subject>692/699/1670/316/801</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNptkV9rHCEUxaW0NCHJQ79Amce2MI1eZ3fHl0D-tGkgNFDaZ7nqnV2XWZ3oTCHfPmY3XRKoIF7u-XH0ehj7IPhXwWV7mhMNsJBy_oYdAm9mNUiAty_qA3aS85qXNQPVCPWeHcACZqKdy0PW34SVN370MVSxq-7ySNH2mMfqyncdJQqjx62KwVUXMVD1i3JMw7ZnHqoLn4dVfNoBR6ptDOtpWSpXXcfeVT8xxAHT6G1P-Zi967DPdPJ8HrE_37_9vvxR395d31ye39a2EXysDRprsTFGcrtw6NB2jRKNcAqAW2obDq0BMGC5Ey1Z5xDBkOq4UArmUh6xs53vMJkNOVuGSNjrIfkNpgcd0evXSvArvYx_ddMqDkoUg0_PBineT5RHvfHZUt9joDhlLRZKbsl5QT_vUJtiLll0-2sE108B6X1Ahf348l178l8cBfiyA3KRwpKSXscphfJX_3F7BCYdnlc</recordid><startdate>20160602</startdate><enddate>20160602</enddate><creator>Lee, Donghyun</creator><creator>Heo, Dong Nyoung</creator><creator>Kim, Han-Jun</creator><creator>Ko, Wan-Kyu</creator><creator>Lee, Sang Jin</creator><creator>Heo, Min</creator><creator>Bang, Jae Beum</creator><creator>Lee, Jung Bok</creator><creator>Hwang, Deok-Sang</creator><creator>Do, Sun Hee</creator><creator>Kwon, Il Keun</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160602</creationdate><title>Inhibition of Osteoclast Differentiation and Bone Resorption by Bisphosphonate-conjugated Gold Nanoparticles</title><author>Lee, Donghyun ; Heo, Dong Nyoung ; Kim, Han-Jun ; Ko, Wan-Kyu ; Lee, Sang Jin ; Heo, Min ; Bang, Jae Beum ; Lee, Jung Bok ; Hwang, Deok-Sang ; Do, Sun Hee ; Kwon, Il Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-babcca4bb30c7dadacf49141d9220ce84028b22b2c0d18ecddaa2be9f01992633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>101/28</topic><topic>14/63</topic><topic>631/1647/350/354</topic><topic>692/699/1670/316/801</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Donghyun</creatorcontrib><creatorcontrib>Heo, Dong Nyoung</creatorcontrib><creatorcontrib>Kim, Han-Jun</creatorcontrib><creatorcontrib>Ko, Wan-Kyu</creatorcontrib><creatorcontrib>Lee, Sang Jin</creatorcontrib><creatorcontrib>Heo, Min</creatorcontrib><creatorcontrib>Bang, Jae Beum</creatorcontrib><creatorcontrib>Lee, Jung Bok</creatorcontrib><creatorcontrib>Hwang, Deok-Sang</creatorcontrib><creatorcontrib>Do, Sun Hee</creatorcontrib><creatorcontrib>Kwon, Il Keun</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Donghyun</au><au>Heo, Dong Nyoung</au><au>Kim, Han-Jun</au><au>Ko, Wan-Kyu</au><au>Lee, Sang Jin</au><au>Heo, Min</au><au>Bang, Jae Beum</au><au>Lee, Jung Bok</au><au>Hwang, Deok-Sang</au><au>Do, Sun Hee</au><au>Kwon, Il Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Osteoclast Differentiation and Bone Resorption by Bisphosphonate-conjugated Gold Nanoparticles</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-06-02</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>27336</spage><epage>27336</epage><pages>27336-27336</pages><artnum>27336</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In recent years, gold nanoparticles (GNPs) have been reported to affect the regeneration of bone tissue. The goal of this study was to improve bone tissue regeneration by using targeted GNPs. We fabricated a functionalized GNPs conjugated with alendronate (ALD), of the bisphosphonate group. Subsequently, the ALD, GNPs, and ALD conjugated GNPs (GNPs-ALD) were analyzed by ultraviolet-visible absorbance (UV-vis) spectrophotometer, Attenuated total reflectance Fourier transform infrared spectrometer (ATR-FTIR), and thermo gravimetric analysis (TGA). The prepared GNPs-ALD were used to investigate their inhibitory effects on the receptor activator of nuclear factor- κb ligand (RANKL)-induced osteoclastogenesis in bone marrow-derived macrophages (BMMs). Additionally, the GNPs-ALD were applied to ovariectomy (OVX)-induced osteoporotic mice and the experiments were evaluated. ALD was found to be successfully conjugated to the GNPs surface, and it displayed significant adhesion onto the bone surface. The
in-vitro
study indicated that the GNPs, ALD and GNPs-ALD suppressed osteoclast formation in a dose-dependent manner. Furthermore, in the OVX mouse model, the mice treated GNPs-ALD had higher bone density as compared to other OVX mice groups. The results from these tests indicated that GNPs-ALD can be useful agents for preventing and treating osteoporosis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27251863</pmid><doi>10.1038/srep27336</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 101/28 14/63 631/1647/350/354 692/699/1670/316/801 Humanities and Social Sciences multidisciplinary Science Science (multidisciplinary) |
title | Inhibition of Osteoclast Differentiation and Bone Resorption by Bisphosphonate-conjugated Gold Nanoparticles |
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