ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila
The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC w...
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| Veröffentlicht in: | Nucleic acids research 2016-06, Vol.44 (10), p.4920-4933 |
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| creator | Kopytova, Daria Popova, Varvara Kurshakova, Maria Shidlovskii, Yulii Nabirochkina, Elena Brechalov, Alexander Georgiev, Georgii Georgieva, Sofia |
| description | The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export. |
| doi_str_mv | 10.1093/nar/gkw192 |
| format | Article |
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In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gkw192</identifier><identifier>PMID: 27016737</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject><![CDATA[Animals ; Cell Nucleus - metabolism ; Drosophila ; Drosophila - genetics ; Drosophila - metabolism ; Drosophila Proteins - antagonists & inhibitors ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Nuclear Proteins - metabolism ; Nucleocytoplasmic Transport Proteins - antagonists & inhibitors ; Nucleocytoplasmic Transport Proteins - genetics ; Nucleocytoplasmic Transport Proteins - isolation & purification ; Nucleocytoplasmic Transport Proteins - metabolism ; Origin Recognition Complex - antagonists & inhibitors ; Origin Recognition Complex - genetics ; Origin Recognition Complex - metabolism ; Protein Subunits - antagonists & inhibitors ; Protein Subunits - genetics ; Protein Subunits - isolation & purification ; Protein Subunits - metabolism ; Ribonucleoproteins - metabolism ; RNA ; RNA Interference ; RNA Transport ; RNA, Messenger - metabolism ; RNA-Binding Proteins - antagonists & inhibitors ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism]]></subject><ispartof>Nucleic acids research, 2016-06, Vol.44 (10), p.4920-4933</ispartof><rights>The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.</rights><rights>The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-6206c513c897a1effc4a9b1a8300484e6a16f94c917dea64f2f15927fd5915503</citedby><cites>FETCH-LOGICAL-c375t-6206c513c897a1effc4a9b1a8300484e6a16f94c917dea64f2f15927fd5915503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889942/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889942/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27016737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kopytova, Daria</creatorcontrib><creatorcontrib>Popova, Varvara</creatorcontrib><creatorcontrib>Kurshakova, Maria</creatorcontrib><creatorcontrib>Shidlovskii, Yulii</creatorcontrib><creatorcontrib>Nabirochkina, Elena</creatorcontrib><creatorcontrib>Brechalov, Alexander</creatorcontrib><creatorcontrib>Georgiev, Georgii</creatorcontrib><creatorcontrib>Georgieva, Sofia</creatorcontrib><title>ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.</description><subject>Animals</subject><subject>Cell Nucleus - metabolism</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - antagonists & inhibitors</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nucleocytoplasmic Transport Proteins - antagonists & inhibitors</subject><subject>Nucleocytoplasmic Transport Proteins - genetics</subject><subject>Nucleocytoplasmic Transport Proteins - isolation & purification</subject><subject>Nucleocytoplasmic Transport Proteins - metabolism</subject><subject>Origin Recognition Complex - antagonists & inhibitors</subject><subject>Origin Recognition Complex - genetics</subject><subject>Origin Recognition Complex - metabolism</subject><subject>Protein Subunits - antagonists & inhibitors</subject><subject>Protein Subunits - genetics</subject><subject>Protein Subunits - isolation & purification</subject><subject>Protein Subunits - metabolism</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA Transport</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-Binding Proteins - antagonists & inhibitors</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEQxS0EoqFw4QOgPSKkJR7_XV-QqlAoUtWiECRuluPYjWF3vdheWr49LikVnDjNSPObp3nzEHoO-DVgRZejScurb9egyAO0ACpIy5QgD9ECU8xbwKw7Qk9y_ooxMODsMToiEoOQVC7QdLleNWEsLhlbcnMdyr7ZnH1aLTfr0y8tacy4a0Id5Hk7j7fNlOIQi2subjw0JudogykhjofVYX3x8fdObU4adzPFVKp88zbFHKd96M1T9MibPrtnd_UYfX53ulmdteeX7z-sTs5bSyUvrSBYWA7UdkoacN5bZtQWTEdx9cOcMCC8YlaB3DkjmCceuCLS77gCzjE9Rm8OutO8HdzOurEk0-sphcGknzqaoP-djGGvr-IPzbpOKUaqwMs7gRS_zy4XPYRsXd-b0cU5a-hwJ0Bwgf-PSkUVppKJir46oLZ-JCfn7y8CrG_T1DVNfUizwi_-9nCP_omP_gJIAJuR</recordid><startdate>20160602</startdate><enddate>20160602</enddate><creator>Kopytova, Daria</creator><creator>Popova, Varvara</creator><creator>Kurshakova, Maria</creator><creator>Shidlovskii, Yulii</creator><creator>Nabirochkina, Elena</creator><creator>Brechalov, Alexander</creator><creator>Georgiev, Georgii</creator><creator>Georgieva, Sofia</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20160602</creationdate><title>ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila</title><author>Kopytova, Daria ; Popova, Varvara ; Kurshakova, Maria ; Shidlovskii, Yulii ; Nabirochkina, Elena ; Brechalov, Alexander ; Georgiev, Georgii ; Georgieva, Sofia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-6206c513c897a1effc4a9b1a8300484e6a16f94c917dea64f2f15927fd5915503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Cell Nucleus - metabolism</topic><topic>Drosophila</topic><topic>Drosophila - genetics</topic><topic>Drosophila - metabolism</topic><topic>Drosophila Proteins - antagonists & inhibitors</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nucleocytoplasmic Transport Proteins - antagonists & inhibitors</topic><topic>Nucleocytoplasmic Transport Proteins - genetics</topic><topic>Nucleocytoplasmic Transport Proteins - isolation & purification</topic><topic>Nucleocytoplasmic Transport Proteins - metabolism</topic><topic>Origin Recognition Complex - antagonists & inhibitors</topic><topic>Origin Recognition Complex - genetics</topic><topic>Origin Recognition Complex - metabolism</topic><topic>Protein Subunits - antagonists & inhibitors</topic><topic>Protein Subunits - genetics</topic><topic>Protein Subunits - isolation & purification</topic><topic>Protein Subunits - metabolism</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA Transport</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-Binding Proteins - antagonists & inhibitors</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kopytova, Daria</creatorcontrib><creatorcontrib>Popova, Varvara</creatorcontrib><creatorcontrib>Kurshakova, Maria</creatorcontrib><creatorcontrib>Shidlovskii, Yulii</creatorcontrib><creatorcontrib>Nabirochkina, Elena</creatorcontrib><creatorcontrib>Brechalov, Alexander</creatorcontrib><creatorcontrib>Georgiev, Georgii</creatorcontrib><creatorcontrib>Georgieva, Sofia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kopytova, Daria</au><au>Popova, Varvara</au><au>Kurshakova, Maria</au><au>Shidlovskii, Yulii</au><au>Nabirochkina, Elena</au><au>Brechalov, Alexander</au><au>Georgiev, Georgii</au><au>Georgieva, Sofia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2016-06-02</date><risdate>2016</risdate><volume>44</volume><issue>10</issue><spage>4920</spage><epage>4933</epage><pages>4920-4933</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates the pre-replication complex assembly. In the literature, there are several reports of interaction of ORC with different RNAs. Here, we demonstrate for the first time a direct interaction of ORC with the THSC/TREX-2 mRNA nuclear export complex. The THSC/TREX-2 was purified from the Drosophila embryonic extract and found to bind with a fraction of the ORC. This interaction occurred via several subunits and was essential for Drosophila viability. Also, ORC was associated with mRNP, which was facilitated by TREX-2. ORC subunits interacted with the Nxf1 receptor mediating the bulk mRNA export. The knockdown of Orc5 led to a drop in the Nxf1 association with mRNP, while Orc3 knockdown increased the level of mRNP-bound Nxf1. The knockdown of Orc5, Orc3 and several other ORC subunits led to an accumulation of mRNA in the nucleus, suggesting that ORC participates in the regulation of the mRNP export.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27016737</pmid><doi>10.1093/nar/gkw192</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Nucleus - metabolism Drosophila Drosophila - genetics Drosophila - metabolism Drosophila Proteins - antagonists & inhibitors Drosophila Proteins - genetics Drosophila Proteins - metabolism Nuclear Proteins - metabolism Nucleocytoplasmic Transport Proteins - antagonists & inhibitors Nucleocytoplasmic Transport Proteins - genetics Nucleocytoplasmic Transport Proteins - isolation & purification Nucleocytoplasmic Transport Proteins - metabolism Origin Recognition Complex - antagonists & inhibitors Origin Recognition Complex - genetics Origin Recognition Complex - metabolism Protein Subunits - antagonists & inhibitors Protein Subunits - genetics Protein Subunits - isolation & purification Protein Subunits - metabolism Ribonucleoproteins - metabolism RNA RNA Interference RNA Transport RNA, Messenger - metabolism RNA-Binding Proteins - antagonists & inhibitors RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism |
| title | ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association with mRNP and mRNA export in Drosophila |
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