The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice
Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccin...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2016-04, Vol.48 (4), p.385-390 |
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| creator | Gu, Dongqing Chen, Wei Mi, Youjun Gong, Xueli Luo, Tao Bao, Lang |
| description | Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein- and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB. |
| doi_str_mv | 10.1093/abbs/gmw010 |
| format | Article |
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In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein- and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmw010</identifier><identifier>PMID: 26922320</identifier><language>eng</language><publisher>China: Oxford University Press</publisher><subject>Animals ; Antibodies, Bacterial - blood ; BCG Vaccine - administration & dosage ; BCG Vaccine - immunology ; Female ; Mice ; Mice, Inbred BALB C ; Mycobacterium bovis - immunology ; Original ; Th1 Cells - classification ; Th1 Cells - immunology ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology</subject><ispartof>Acta biochimica et biophysica Sinica, 2016-04, Vol.48 (4), p.385-390</ispartof><rights>The Author 2016. 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In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein- and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>BCG Vaccine - administration & dosage</subject><subject>BCG Vaccine - immunology</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mycobacterium bovis - immunology</subject><subject>Original</subject><subject>Th1 Cells - classification</subject><subject>Th1 Cells - immunology</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtr3DAUhUVpaR7tKvuiZaE40cuSvAkk07wgbaFM10KSrzMqtjWR7An599EwSUhW98L5OOdeDkJHlBxT0vAT61w-uRseCCUf0D5Voq4UU-Rj2aViVUNFvYcOcv5PCJeSks9oj8mGMc7IPuqXK8C_Hn101k-QwjxgFzch4_PFFV6nMED1d0NqpfDP32dFinnCG-t9GO0U4ojD2M4eMra4nZN1PeDliuIwDPMIOEFexzFDofAQPHxBnzrbZ_j6PA_Rv8uL5eK6uv1zdbM4u608F2KqrPCdkratGZNUdbIjWvCmTOed81q71nNSW94QrbvOaSWJctoDd9IpzgQ_RKc73_XsBmg9jFOyvdm-Y9OjiTaY98oYVuYubozQWjLRFIPvzwYp3s-QJzOE7KHv7QhxzoaqklnYRhX0xw71KeacoHuNocRs-zHbfsyun0J_e3vZK_tSCH8C3QGODA</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Gu, Dongqing</creator><creator>Chen, Wei</creator><creator>Mi, Youjun</creator><creator>Gong, Xueli</creator><creator>Luo, Tao</creator><creator>Bao, Lang</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160401</creationdate><title>The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice</title><author>Gu, Dongqing ; Chen, Wei ; Mi, Youjun ; Gong, Xueli ; Luo, Tao ; Bao, Lang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-a4cf76ad522617f6f08439f6fbcbbc88bdc305a39088ffb87607b8ce3b6b73243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>BCG Vaccine - administration & dosage</topic><topic>BCG Vaccine - immunology</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mycobacterium bovis - immunology</topic><topic>Original</topic><topic>Th1 Cells - classification</topic><topic>Th1 Cells - immunology</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Dongqing</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Mi, Youjun</creatorcontrib><creatorcontrib>Gong, Xueli</creatorcontrib><creatorcontrib>Luo, Tao</creatorcontrib><creatorcontrib>Bao, Lang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Dongqing</au><au>Chen, Wei</au><au>Mi, Youjun</au><au>Gong, Xueli</au><au>Luo, Tao</au><au>Bao, Lang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochim Biophys Sin (Shanghai)</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>48</volume><issue>4</issue><spage>385</spage><epage>390</epage><pages>385-390</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially enhance the immunogenicity of BCG and strengthen both CD4+ T cell-mediated Th1 and CD8+ T cell-mediated cytolytic responses. Compared with the protein-based vaccine, the DNA-based vaccine can induce more durable Th1 immune response, characterized by high levels of antibody response, proliferation response, percentages of CD4+/CD8+ and cytokine secretion in antigen-stimulated splenocyte cultures. In conclusion, we for the first time, developed a protein- and plasmid DNA-based booster vaccine based on Rv0577. Our findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.</abstract><cop>China</cop><pub>Oxford University Press</pub><pmid>26922320</pmid><doi>10.1093/abbs/gmw010</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Bacterial - blood BCG Vaccine - administration & dosage BCG Vaccine - immunology Female Mice Mice, Inbred BALB C Mycobacterium bovis - immunology Original Th1 Cells - classification Th1 Cells - immunology Vaccines, DNA - administration & dosage Vaccines, DNA - immunology |
| title | The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice |
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