Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu)
Glutathione peroxidase-1 (GPX-1) activity was reported to be useful marker for monitoring cardiovascular disease. However, accurate assessment of coronary artery disease (CAD) using GPX-1 polymorphism is limited for South Asian population. Present study aim to assess GPX-1activity and GPX-1 polymorp...
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| Veröffentlicht in: | BMC cardiovascular disorders 2016-05, Vol.16 (1), p.111-111, Article 111 |
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| creator | Wickremasinghe, Dinushka Peiris, Hemantha Chandrasena, Lal Gotabhaya Senaratne, Vajira Perera, Rasika |
| description | Glutathione peroxidase-1 (GPX-1) activity was reported to be useful marker for monitoring cardiovascular disease. However, accurate assessment of coronary artery disease (CAD) using GPX-1 polymorphism is limited for South Asian population. Present study aim to assess GPX-1activity and GPX-1 polymorphismin patients with coronary artery disease (CAD) who were confirmed with coronary angiography findings and in apparently healthy subjects.
Case control study was carried out with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having CAD on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for erythrocyte GPX-1 activity and GPX-1 polymorphism in both groups and the severity of CAD was assessed using coronary angiography scoring system based on vessel, stenosis and extent score.
Coronary angiography scores indicated that erythrocyteGPX-1 cutoff value of 23.9 U/gHb showed a high sensitivity and negative predictive value in ruling out major vessel disease. The GPX-1 Pro198Leu (CT) polymorphism was higher in patients with CAD (25.3 %) when compared to controls (10.7 %). Pro198Leu (CT) genotype showed a 2.84 fold risk for CAD [odds ratio 2.84 (95 % CI 1.15-6.98), p = 0.019].
Coronary angiography findings indicated that individuals possessing Pro198Leu (CT) polymorphism were found to be associated with low erythrocyte GPX-1 activity and increased susceptibility for CAD. |
| doi_str_mv | 10.1186/s12872-016-0280-9 |
| format | Article |
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Case control study was carried out with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having CAD on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for erythrocyte GPX-1 activity and GPX-1 polymorphism in both groups and the severity of CAD was assessed using coronary angiography scoring system based on vessel, stenosis and extent score.
Coronary angiography scores indicated that erythrocyteGPX-1 cutoff value of 23.9 U/gHb showed a high sensitivity and negative predictive value in ruling out major vessel disease. The GPX-1 Pro198Leu (CT) polymorphism was higher in patients with CAD (25.3 %) when compared to controls (10.7 %). Pro198Leu (CT) genotype showed a 2.84 fold risk for CAD [odds ratio 2.84 (95 % CI 1.15-6.98), p = 0.019].
Coronary angiography findings indicated that individuals possessing Pro198Leu (CT) polymorphism were found to be associated with low erythrocyte GPX-1 activity and increased susceptibility for CAD.</description><identifier>ISSN: 1471-2261</identifier><identifier>EISSN: 1471-2261</identifier><identifier>DOI: 10.1186/s12872-016-0280-9</identifier><identifier>PMID: 27229152</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Adult ; Case-Control Studies ; Chi-Square Distribution ; Coronary Angiography ; Coronary Artery Disease - blood ; Coronary Artery Disease - diagnosis ; Coronary Artery Disease - enzymology ; Coronary Artery Disease - genetics ; Coronary Stenosis - blood ; Coronary Stenosis - diagnosis ; Coronary Stenosis - enzymology ; Coronary Stenosis - genetics ; Erythrocytes - enzymology ; Feasibility Studies ; Female ; Gene Frequency ; Genetic Association Studies ; Genetic Predisposition to Disease ; Glutathione Peroxidase - blood ; Glutathione Peroxidase - genetics ; Humans ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Odds Ratio ; Phenotype ; Polymorphism, Genetic ; Risk Factors ; Severity of Illness Index</subject><ispartof>BMC cardiovascular disorders, 2016-05, Vol.16 (1), p.111-111, Article 111</ispartof><rights>Copyright BioMed Central 2016</rights><rights>The Author(s). 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-2f8172f247f51f523ef048399dbf8f5a0205bc4f726fb028c62ab0eef9f957c53</citedby><cites>FETCH-LOGICAL-c427t-2f8172f247f51f523ef048399dbf8f5a0205bc4f726fb028c62ab0eef9f957c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882825/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882825/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27229152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wickremasinghe, Dinushka</creatorcontrib><creatorcontrib>Peiris, Hemantha</creatorcontrib><creatorcontrib>Chandrasena, Lal Gotabhaya</creatorcontrib><creatorcontrib>Senaratne, Vajira</creatorcontrib><creatorcontrib>Perera, Rasika</creatorcontrib><title>Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu)</title><title>BMC cardiovascular disorders</title><addtitle>BMC Cardiovasc Disord</addtitle><description>Glutathione peroxidase-1 (GPX-1) activity was reported to be useful marker for monitoring cardiovascular disease. However, accurate assessment of coronary artery disease (CAD) using GPX-1 polymorphism is limited for South Asian population. Present study aim to assess GPX-1activity and GPX-1 polymorphismin patients with coronary artery disease (CAD) who were confirmed with coronary angiography findings and in apparently healthy subjects.
Case control study was carried out with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having CAD on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for erythrocyte GPX-1 activity and GPX-1 polymorphism in both groups and the severity of CAD was assessed using coronary angiography scoring system based on vessel, stenosis and extent score.
Coronary angiography scores indicated that erythrocyteGPX-1 cutoff value of 23.9 U/gHb showed a high sensitivity and negative predictive value in ruling out major vessel disease. The GPX-1 Pro198Leu (CT) polymorphism was higher in patients with CAD (25.3 %) when compared to controls (10.7 %). Pro198Leu (CT) genotype showed a 2.84 fold risk for CAD [odds ratio 2.84 (95 % CI 1.15-6.98), p = 0.019].
Coronary angiography findings indicated that individuals possessing Pro198Leu (CT) polymorphism were found to be associated with low erythrocyte GPX-1 activity and increased susceptibility for CAD.</description><subject>Adult</subject><subject>Case-Control Studies</subject><subject>Chi-Square Distribution</subject><subject>Coronary Angiography</subject><subject>Coronary Artery Disease - blood</subject><subject>Coronary Artery Disease - diagnosis</subject><subject>Coronary Artery Disease - enzymology</subject><subject>Coronary Artery Disease - genetics</subject><subject>Coronary Stenosis - blood</subject><subject>Coronary Stenosis - diagnosis</subject><subject>Coronary Stenosis - enzymology</subject><subject>Coronary Stenosis - genetics</subject><subject>Erythrocytes - enzymology</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Genetic Association Studies</subject><subject>Genetic Predisposition to Disease</subject><subject>Glutathione Peroxidase - blood</subject><subject>Glutathione Peroxidase - genetics</subject><subject>Humans</subject><subject>Logistic Models</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Multivariate Analysis</subject><subject>Odds Ratio</subject><subject>Phenotype</subject><subject>Polymorphism, Genetic</subject><subject>Risk Factors</subject><subject>Severity of Illness Index</subject><issn>1471-2261</issn><issn>1471-2261</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdUk1v1DAQjRCIlsIP4IIscdkeUuzJh-0LElqVpdJK7AEkbpaTjBtXSbzYTsv-qP5HHLZUhdOMNe89zRu_LHvL6AVjov4QGAgOOWV1TkHQXD7LTlnJWQ5Qs-dP-pPsVQg3lDIuqHyZnQAHkKyC0-x-rQOS1k3Ru4EY1ME2drDxQEKcuwPRIWAIdromscfl5Vqro3UTaTDeIU4k4C36heBM0vFu0j7RfMRUOhtw0b-zsSebYY469omLZIfe_bJdmuWMrDa7Hzk7J3rqyJ-W7N1wGJ3f9zaMZLXzjkmxxfn8dfbC6CHgm4d6ln3_fPlt_SXfft1crT9t87YEHnMwgnEwUHJTMVNBgYaWopCya4wwlaZAq6YtDYfaNOlybQ26oYhGGlnxtirOso9H3f3cjNi1mM6jB7X3dkzulNNW_TuZbK-u3a0qhQABi8DqQcC7nzOGqEYbWhwGPaGbg2JcQsE5reoEff8f9MbNfkr2FhQvaA2iSCh2RLXeheDRPC7DqFrCoI5hUCkMagmDkonz7qmLR8bf3y9-Ax0Aspw</recordid><startdate>20160526</startdate><enddate>20160526</enddate><creator>Wickremasinghe, Dinushka</creator><creator>Peiris, Hemantha</creator><creator>Chandrasena, Lal Gotabhaya</creator><creator>Senaratne, Vajira</creator><creator>Perera, Rasika</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160526</creationdate><title>Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu)</title><author>Wickremasinghe, Dinushka ; 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However, accurate assessment of coronary artery disease (CAD) using GPX-1 polymorphism is limited for South Asian population. Present study aim to assess GPX-1activity and GPX-1 polymorphismin patients with coronary artery disease (CAD) who were confirmed with coronary angiography findings and in apparently healthy subjects.
Case control study was carried out with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having CAD on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for erythrocyte GPX-1 activity and GPX-1 polymorphism in both groups and the severity of CAD was assessed using coronary angiography scoring system based on vessel, stenosis and extent score.
Coronary angiography scores indicated that erythrocyteGPX-1 cutoff value of 23.9 U/gHb showed a high sensitivity and negative predictive value in ruling out major vessel disease. The GPX-1 Pro198Leu (CT) polymorphism was higher in patients with CAD (25.3 %) when compared to controls (10.7 %). Pro198Leu (CT) genotype showed a 2.84 fold risk for CAD [odds ratio 2.84 (95 % CI 1.15-6.98), p = 0.019].
Coronary angiography findings indicated that individuals possessing Pro198Leu (CT) polymorphism were found to be associated with low erythrocyte GPX-1 activity and increased susceptibility for CAD.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>27229152</pmid><doi>10.1186/s12872-016-0280-9</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Case-Control Studies Chi-Square Distribution Coronary Angiography Coronary Artery Disease - blood Coronary Artery Disease - diagnosis Coronary Artery Disease - enzymology Coronary Artery Disease - genetics Coronary Stenosis - blood Coronary Stenosis - diagnosis Coronary Stenosis - enzymology Coronary Stenosis - genetics Erythrocytes - enzymology Feasibility Studies Female Gene Frequency Genetic Association Studies Genetic Predisposition to Disease Glutathione Peroxidase - blood Glutathione Peroxidase - genetics Humans Logistic Models Male Middle Aged Multivariate Analysis Odds Ratio Phenotype Polymorphism, Genetic Risk Factors Severity of Illness Index |
| title | Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu) |
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