Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test
We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (U...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2016, Vol.78(4), pp.723-725 |
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| creator | MORIOKA, Ayako SHIMAZAKI, Yoko UCHIYAMA, Mariko SUZUKI, Shoko |
| description | We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction temperature to determine heat influence. UT-serovar 1 and 15 were influenced by heat, because their precipitation lines were observed in the case of low antigen extraction temperature. To investigate the relationship between antigenicity and genotype, we performed pulsed-field gel electrophoresis (PFGE) analysis using UT-serovar 2 and 15. The predominant PFGE pattern of UT-serovar 2 was identical to that of serovar 2. |
| doi_str_mv | 10.1292/jvms.15-0538 |
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To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction temperature to determine heat influence. UT-serovar 1 and 15 were influenced by heat, because their precipitation lines were observed in the case of low antigen extraction temperature. To investigate the relationship between antigenicity and genotype, we performed pulsed-field gel electrophoresis (PFGE) analysis using UT-serovar 2 and 15. The predominant PFGE pattern of UT-serovar 2 was identical to that of serovar 2.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.15-0538</identifier><identifier>PMID: 26726101</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Actinobacillus Infections - microbiology ; Actinobacillus Infections - veterinary ; Actinobacillus pleuropneumoniae ; Actinobacillus pleuropneumoniae - classification ; Actinobacillus pleuropneumoniae - genetics ; Actinobacillus pleuropneumoniae - immunology ; Agglutination Tests - veterinary ; Animals ; Bacteriology ; Electrophoresis, Gel, Pulsed-Field - veterinary ; Immunodiffusion - veterinary ; Multiplex Polymerase Chain Reaction - veterinary ; Pleuropneumonia - microbiology ; Pleuropneumonia - veterinary ; serotyping ; Serotyping - methods ; Serotyping - veterinary ; Swine ; Swine Diseases - microbiology</subject><ispartof>Journal of Veterinary Medical Science, 2016, Vol.78(4), pp.723-725</ispartof><rights>2016 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><rights>2016 The Japanese Society of Veterinary Science 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c722t-343199d00da0743123c465e24e2a96bae326c78d891d8547fdf9e92a0141df0c3</citedby><cites>FETCH-LOGICAL-c722t-343199d00da0743123c465e24e2a96bae326c78d891d8547fdf9e92a0141df0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873870/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873870/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,1877,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26726101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MORIOKA, Ayako</creatorcontrib><creatorcontrib>SHIMAZAKI, Yoko</creatorcontrib><creatorcontrib>UCHIYAMA, Mariko</creatorcontrib><creatorcontrib>SUZUKI, Shoko</creatorcontrib><title>Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction temperature to determine heat influence. UT-serovar 1 and 15 were influenced by heat, because their precipitation lines were observed in the case of low antigen extraction temperature. To investigate the relationship between antigenicity and genotype, we performed pulsed-field gel electrophoresis (PFGE) analysis using UT-serovar 2 and 15. The predominant PFGE pattern of UT-serovar 2 was identical to that of serovar 2.</description><subject>Actinobacillus Infections - microbiology</subject><subject>Actinobacillus Infections - veterinary</subject><subject>Actinobacillus pleuropneumoniae</subject><subject>Actinobacillus pleuropneumoniae - classification</subject><subject>Actinobacillus pleuropneumoniae - genetics</subject><subject>Actinobacillus pleuropneumoniae - immunology</subject><subject>Agglutination Tests - veterinary</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>Immunodiffusion - veterinary</subject><subject>Multiplex Polymerase Chain Reaction - veterinary</subject><subject>Pleuropneumonia - microbiology</subject><subject>Pleuropneumonia - veterinary</subject><subject>serotyping</subject><subject>Serotyping - methods</subject><subject>Serotyping - veterinary</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EotuFG2dkiQsHUmzHie0LUltRQKrEAThbjjNJHTn2YieV9t-TKMsKOHGZObxPT_PmIfSKkivKFHs_PI75ilYFqUr5BO1oyUUheKmeoh1RtC4Eq8gFusx5IIRRXqvn6ILVgtWU0B0avkGK0_HgQo8TmGD8MbuMY4fnkDfJNB7wtZ1ciI2xzvs544OHOcVDgHmMwRnALkdvJsi4OWLTm4R78Lh1XTdnFwNelOkFetYZn-Hlae_Rj7uP328_F_dfP325vb4vrGBsKkpeUqVaQlpDlhyUlZbXFTAOzKi6MVCy2grZSkVbWXHRtZ0CxQyhnLYdseUefdh8D3MzQmshTMl4fUhuNOmoo3H6byW4B93HR82lKKUgi8Hbk0GKP-flcj26bMF7EyDOWVOhOFeyquh_oFJwSaqaL-ibf9Ahzml5-EYRJtase_Ruo2yKOSfozndTote-9dq3ppVe-17w139mPcO_C16Amw0Y8mR6OAMmTc562NyE1HwdJ9ezaB9M0hDKX1ttwPk</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>MORIOKA, Ayako</creator><creator>SHIMAZAKI, Yoko</creator><creator>UCHIYAMA, Mariko</creator><creator>SUZUKI, Shoko</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test</title><author>MORIOKA, Ayako ; SHIMAZAKI, Yoko ; UCHIYAMA, Mariko ; SUZUKI, Shoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c722t-343199d00da0743123c465e24e2a96bae326c78d891d8547fdf9e92a0141df0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Actinobacillus Infections - microbiology</topic><topic>Actinobacillus Infections - veterinary</topic><topic>Actinobacillus pleuropneumoniae</topic><topic>Actinobacillus pleuropneumoniae - classification</topic><topic>Actinobacillus pleuropneumoniae - genetics</topic><topic>Actinobacillus pleuropneumoniae - immunology</topic><topic>Agglutination Tests - veterinary</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>Immunodiffusion - veterinary</topic><topic>Multiplex Polymerase Chain Reaction - veterinary</topic><topic>Pleuropneumonia - microbiology</topic><topic>Pleuropneumonia - veterinary</topic><topic>serotyping</topic><topic>Serotyping - methods</topic><topic>Serotyping - veterinary</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MORIOKA, Ayako</creatorcontrib><creatorcontrib>SHIMAZAKI, Yoko</creatorcontrib><creatorcontrib>UCHIYAMA, Mariko</creatorcontrib><creatorcontrib>SUZUKI, Shoko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MORIOKA, Ayako</au><au>SHIMAZAKI, Yoko</au><au>UCHIYAMA, Mariko</au><au>SUZUKI, Shoko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>78</volume><issue>4</issue><spage>723</spage><epage>725</epage><pages>723-725</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction temperature to determine heat influence. UT-serovar 1 and 15 were influenced by heat, because their precipitation lines were observed in the case of low antigen extraction temperature. To investigate the relationship between antigenicity and genotype, we performed pulsed-field gel electrophoresis (PFGE) analysis using UT-serovar 2 and 15. 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| subjects | Actinobacillus Infections - microbiology Actinobacillus Infections - veterinary Actinobacillus pleuropneumoniae Actinobacillus pleuropneumoniae - classification Actinobacillus pleuropneumoniae - genetics Actinobacillus pleuropneumoniae - immunology Agglutination Tests - veterinary Animals Bacteriology Electrophoresis, Gel, Pulsed-Field - veterinary Immunodiffusion - veterinary Multiplex Polymerase Chain Reaction - veterinary Pleuropneumonia - microbiology Pleuropneumonia - veterinary serotyping Serotyping - methods Serotyping - veterinary Swine Swine Diseases - microbiology |
| title | Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test |
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