Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina
Programmed cell death occurs naturally at different stages of neural development, including neurogenesis. The functional role of this early phase of neural cell death, which affects recently differentiated neurons among other cell types, remains undefined. Some mouse models defective in DNA double-s...
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description | Programmed cell death occurs naturally at different stages of neural development, including neurogenesis. The functional role of this early phase of neural cell death, which affects recently differentiated neurons among other cell types, remains undefined. Some mouse models defective in DNA double-strand break (DSB) repair present massive cell death during neural development, occasionally provoking embryonic lethality, while other organs and tissues remain unaffected. This suggests that DSBs occur frequently and selectively in the developing nervous system. We analyzed the embryonic retina of a mouse model deficient in the error-prone DNA polymerase μ (Polμ), a key component of the non-homologous end-joining (NHEJ) repair system. DNA DSBs were increased in the mutant mouse at embryonic day 13.5 (E13.5), as well as the incidence of cell death that affected young neurons, including retinal ganglion cells (RGCs). Polμ
−/−
mice also showed disturbed RGC axonal growth and navigation, and altered distribution of the axonal guidance molecules L1-CAM and Bravo (also known as Nr-CAM). These findings demonstrate that Polμ is necessary for proper retinal development, and support that the generation of DSBs and their repair via the NHEJ pathway are genuine processes involved in neural development. |
doi_str_mv | 10.1038/srep25928 |
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−/−
mice also showed disturbed RGC axonal growth and navigation, and altered distribution of the axonal guidance molecules L1-CAM and Bravo (also known as Nr-CAM). These findings demonstrate that Polμ is necessary for proper retinal development, and support that the generation of DSBs and their repair via the NHEJ pathway are genuine processes involved in neural development.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep25928</identifier><identifier>PMID: 27172884</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/2 ; 13/51 ; 631/378 ; 631/378/2571/1696 ; 64/110 ; Humanities and Social Sciences ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2016-05, Vol.6 (1), p.25928-25928, Article 25928</ispartof><rights>The Author(s) 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-69e122ffef76550fcb5c14c4d86d4c2a5f128c2e2b14ce0459b2217eee62bd9f3</citedby><cites>FETCH-LOGICAL-c410t-69e122ffef76550fcb5c14c4d86d4c2a5f128c2e2b14ce0459b2217eee62bd9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865816/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865816/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27172884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baleriola, Jimena</creatorcontrib><creatorcontrib>Álvarez-Lindo, Noemí</creatorcontrib><creatorcontrib>de la Villa, Pedro</creatorcontrib><creatorcontrib>Bernad, Antonio</creatorcontrib><creatorcontrib>Blanco, Luis</creatorcontrib><creatorcontrib>Suárez, Teresa</creatorcontrib><creatorcontrib>de la Rosa, Enrique J.</creatorcontrib><title>Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Programmed cell death occurs naturally at different stages of neural development, including neurogenesis. The functional role of this early phase of neural cell death, which affects recently differentiated neurons among other cell types, remains undefined. Some mouse models defective in DNA double-strand break (DSB) repair present massive cell death during neural development, occasionally provoking embryonic lethality, while other organs and tissues remain unaffected. This suggests that DSBs occur frequently and selectively in the developing nervous system. We analyzed the embryonic retina of a mouse model deficient in the error-prone DNA polymerase μ (Polμ), a key component of the non-homologous end-joining (NHEJ) repair system. DNA DSBs were increased in the mutant mouse at embryonic day 13.5 (E13.5), as well as the incidence of cell death that affected young neurons, including retinal ganglion cells (RGCs). Polμ
−/−
mice also showed disturbed RGC axonal growth and navigation, and altered distribution of the axonal guidance molecules L1-CAM and Bravo (also known as Nr-CAM). These findings demonstrate that Polμ is necessary for proper retinal development, and support that the generation of DSBs and their repair via the NHEJ pathway are genuine processes involved in neural development.</description><subject>13</subject><subject>13/2</subject><subject>13/51</subject><subject>631/378</subject><subject>631/378/2571/1696</subject><subject>64/110</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNptkcFu1DAQhq2qFa3KHniBykdACsSzduJcKlVVgUorwaE9W4493nWV2IudQPtuPAPPhMuWVZHqi635P_0z45-QN6z-wOql_JgTbkF0IA_ICdRcVLAEOHz2PiaLnO_qcgR0nHWvyDG0rAUp-Qnpr4NJqDNaGnBOMeiBWtTThupgqfV5mlNfRH3_V1qn-LNoPtBpg_RbHH7_qiw6bzyGiY5xzkhx7NNDDN7QhJMP-jU5cnrIuHi6T8ntp6ubyy_V6uvn68uLVWU4q6eq6ZABOIeubYSonemFYdxwKxvLDWjhGEgDCH2pYlmu6wFYi4gN9LZzy1NyvvPdzv2I1pSJkh7UNvlRpwcVtVf_K8Fv1Dr-UFw2QrKmGLx9Mkjx-4x5UqPPBodBByybKdbKjncCZFvQdzvUpJhLAm7fhtXqMRa1j6WwZ8_n2pP_QijA-x2QixTWmNRdnFP57vyC2x8AGpp1</recordid><startdate>20160513</startdate><enddate>20160513</enddate><creator>Baleriola, Jimena</creator><creator>Álvarez-Lindo, Noemí</creator><creator>de la Villa, Pedro</creator><creator>Bernad, Antonio</creator><creator>Blanco, Luis</creator><creator>Suárez, Teresa</creator><creator>de la Rosa, Enrique J.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160513</creationdate><title>Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina</title><author>Baleriola, Jimena ; Álvarez-Lindo, Noemí ; de la Villa, Pedro ; Bernad, Antonio ; Blanco, Luis ; Suárez, Teresa ; de la Rosa, Enrique J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-69e122ffef76550fcb5c14c4d86d4c2a5f128c2e2b14ce0459b2217eee62bd9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13</topic><topic>13/2</topic><topic>13/51</topic><topic>631/378</topic><topic>631/378/2571/1696</topic><topic>64/110</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baleriola, Jimena</creatorcontrib><creatorcontrib>Álvarez-Lindo, Noemí</creatorcontrib><creatorcontrib>de la Villa, Pedro</creatorcontrib><creatorcontrib>Bernad, Antonio</creatorcontrib><creatorcontrib>Blanco, Luis</creatorcontrib><creatorcontrib>Suárez, Teresa</creatorcontrib><creatorcontrib>de la Rosa, Enrique J.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baleriola, Jimena</au><au>Álvarez-Lindo, Noemí</au><au>de la Villa, Pedro</au><au>Bernad, Antonio</au><au>Blanco, Luis</au><au>Suárez, Teresa</au><au>de la Rosa, Enrique J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-05-13</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>25928</spage><epage>25928</epage><pages>25928-25928</pages><artnum>25928</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Programmed cell death occurs naturally at different stages of neural development, including neurogenesis. The functional role of this early phase of neural cell death, which affects recently differentiated neurons among other cell types, remains undefined. Some mouse models defective in DNA double-strand break (DSB) repair present massive cell death during neural development, occasionally provoking embryonic lethality, while other organs and tissues remain unaffected. This suggests that DSBs occur frequently and selectively in the developing nervous system. We analyzed the embryonic retina of a mouse model deficient in the error-prone DNA polymerase μ (Polμ), a key component of the non-homologous end-joining (NHEJ) repair system. DNA DSBs were increased in the mutant mouse at embryonic day 13.5 (E13.5), as well as the incidence of cell death that affected young neurons, including retinal ganglion cells (RGCs). Polμ
−/−
mice also showed disturbed RGC axonal growth and navigation, and altered distribution of the axonal guidance molecules L1-CAM and Bravo (also known as Nr-CAM). These findings demonstrate that Polμ is necessary for proper retinal development, and support that the generation of DSBs and their repair via the NHEJ pathway are genuine processes involved in neural development.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27172884</pmid><doi>10.1038/srep25928</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 13 13/2 13/51 631/378 631/378/2571/1696 64/110 Humanities and Social Sciences multidisciplinary Science Science (multidisciplinary) |
title | Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina |
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