The P2X4 receptor is required for neuroprotection via ischemic preconditioning
Ischemic preconditioning (IPC), a procedure consisting of transient ischemia and subsequent reperfusion, provides ischemic tolerance against prolonged ischemia in the brain. Although the blood flow changes mediated by IPC are primarily perceived by vascular endothelial cells, the role of these cells...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2016-05, Vol.6 (1), p.25893-25893, Article 25893 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 25893 |
|---|---|
| container_issue | 1 |
| container_start_page | 25893 |
| container_title | Scientific reports |
| container_volume | 6 |
| creator | Ozaki, Tomohiko Muramatsu, Rieko Sasai, Miwa Yamamoto, Masahiro Kubota, Yoshiaki Fujinaka, Toshiyuki Yoshimine, Toshiki Yamashita, Toshihide |
| description | Ischemic preconditioning (IPC), a procedure consisting of transient ischemia and subsequent reperfusion, provides ischemic tolerance against prolonged ischemia in the brain. Although the blood flow changes mediated by IPC are primarily perceived by vascular endothelial cells, the role of these cells in ischemic tolerance has not been fully clarified. In this study, we found that the P2X4 receptor, which is abundantly expressed in vascular endothelial cells, is required for ischemic tolerance following middle artery occlusion (MCAO) in mice. Mechanistically, the P2X4 receptor was stimulated by fluid shear stress, which mimics reperfusion, thus promoting the increased expression of osteopontin, a neuroprotective molecule. Furthermore, we found that the intracerebroventricular administration of osteopontin was sufficient to exert a neuroprotective effect mediated by preconditioning-stimulated P2X4 receptor activation. These results demonstrate a novel mechanism whereby vascular endothelial cells are involved in ischemic tolerance. |
| doi_str_mv | 10.1038/srep25893 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4865734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1789494240</sourcerecordid><originalsourceid>FETCH-LOGICAL-c560t-240537d2006eb760d22e3a5b71594e3e9da12789116e14cbec806ff73b1bf2f73</originalsourceid><addsrcrecordid>eNptkU1LAzEQhoMottQe_AOyRxVW87kfF0GKXyDqoYK3sJudbVO2yTbZLfjvTWktFcxlJsyTdybvIHRO8A3BLLv1DloqspwdoSHFXMSUUXp8kA_Q2PsFDkfQnJP8FA1oSlKW8WSI3qZziD7oF48cKGg76yLtQ77qtYMqqsPdQO9s62wHqtPWRGtdBEbNYalV1IZn1lR6U9FmdoZO6qLxMN7FEfp8fJhOnuPX96eXyf1rrESCu5hyLFhaUYwTKNMEV5QCK0SZEpFzYJBXBaFplhOSAOGqBJXhpK5TVpKypiGO0N1Wt-3LJVQKTOeKRrZOLwv3LW2h5d-K0XM5s2vJs0SkjAeBy52As6sefCeX4U_QNIUB23tJQnee8zBoQK-2qHLWB7frfRuC5WYFcr-CwF4czrUnfw0PwPUW8KFkZuDkwvbOBK_-UfsBOl2RMg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1789494240</pqid></control><display><type>article</type><title>The P2X4 receptor is required for neuroprotection via ischemic preconditioning</title><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Ozaki, Tomohiko ; Muramatsu, Rieko ; Sasai, Miwa ; Yamamoto, Masahiro ; Kubota, Yoshiaki ; Fujinaka, Toshiyuki ; Yoshimine, Toshiki ; Yamashita, Toshihide</creator><creatorcontrib>Ozaki, Tomohiko ; Muramatsu, Rieko ; Sasai, Miwa ; Yamamoto, Masahiro ; Kubota, Yoshiaki ; Fujinaka, Toshiyuki ; Yoshimine, Toshiki ; Yamashita, Toshihide</creatorcontrib><description>Ischemic preconditioning (IPC), a procedure consisting of transient ischemia and subsequent reperfusion, provides ischemic tolerance against prolonged ischemia in the brain. Although the blood flow changes mediated by IPC are primarily perceived by vascular endothelial cells, the role of these cells in ischemic tolerance has not been fully clarified. In this study, we found that the P2X4 receptor, which is abundantly expressed in vascular endothelial cells, is required for ischemic tolerance following middle artery occlusion (MCAO) in mice. Mechanistically, the P2X4 receptor was stimulated by fluid shear stress, which mimics reperfusion, thus promoting the increased expression of osteopontin, a neuroprotective molecule. Furthermore, we found that the intracerebroventricular administration of osteopontin was sufficient to exert a neuroprotective effect mediated by preconditioning-stimulated P2X4 receptor activation. These results demonstrate a novel mechanism whereby vascular endothelial cells are involved in ischemic tolerance.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep25893</identifier><identifier>PMID: 27173846</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/61 ; 42/109 ; 631/378 ; 64/60 ; 692/617 ; 82/51 ; Humanities and Social Sciences ; multidisciplinary ; Science</subject><ispartof>Scientific reports, 2016-05, Vol.6 (1), p.25893-25893, Article 25893</ispartof><rights>The Author(s) 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-240537d2006eb760d22e3a5b71594e3e9da12789116e14cbec806ff73b1bf2f73</citedby><cites>FETCH-LOGICAL-c560t-240537d2006eb760d22e3a5b71594e3e9da12789116e14cbec806ff73b1bf2f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865734/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865734/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27173846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozaki, Tomohiko</creatorcontrib><creatorcontrib>Muramatsu, Rieko</creatorcontrib><creatorcontrib>Sasai, Miwa</creatorcontrib><creatorcontrib>Yamamoto, Masahiro</creatorcontrib><creatorcontrib>Kubota, Yoshiaki</creatorcontrib><creatorcontrib>Fujinaka, Toshiyuki</creatorcontrib><creatorcontrib>Yoshimine, Toshiki</creatorcontrib><creatorcontrib>Yamashita, Toshihide</creatorcontrib><title>The P2X4 receptor is required for neuroprotection via ischemic preconditioning</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Ischemic preconditioning (IPC), a procedure consisting of transient ischemia and subsequent reperfusion, provides ischemic tolerance against prolonged ischemia in the brain. Although the blood flow changes mediated by IPC are primarily perceived by vascular endothelial cells, the role of these cells in ischemic tolerance has not been fully clarified. In this study, we found that the P2X4 receptor, which is abundantly expressed in vascular endothelial cells, is required for ischemic tolerance following middle artery occlusion (MCAO) in mice. Mechanistically, the P2X4 receptor was stimulated by fluid shear stress, which mimics reperfusion, thus promoting the increased expression of osteopontin, a neuroprotective molecule. Furthermore, we found that the intracerebroventricular administration of osteopontin was sufficient to exert a neuroprotective effect mediated by preconditioning-stimulated P2X4 receptor activation. These results demonstrate a novel mechanism whereby vascular endothelial cells are involved in ischemic tolerance.</description><subject>38/61</subject><subject>42/109</subject><subject>631/378</subject><subject>64/60</subject><subject>692/617</subject><subject>82/51</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Science</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNptkU1LAzEQhoMottQe_AOyRxVW87kfF0GKXyDqoYK3sJudbVO2yTbZLfjvTWktFcxlJsyTdybvIHRO8A3BLLv1DloqspwdoSHFXMSUUXp8kA_Q2PsFDkfQnJP8FA1oSlKW8WSI3qZziD7oF48cKGg76yLtQ77qtYMqqsPdQO9s62wHqtPWRGtdBEbNYalV1IZn1lR6U9FmdoZO6qLxMN7FEfp8fJhOnuPX96eXyf1rrESCu5hyLFhaUYwTKNMEV5QCK0SZEpFzYJBXBaFplhOSAOGqBJXhpK5TVpKypiGO0N1Wt-3LJVQKTOeKRrZOLwv3LW2h5d-K0XM5s2vJs0SkjAeBy52As6sefCeX4U_QNIUB23tJQnee8zBoQK-2qHLWB7frfRuC5WYFcr-CwF4czrUnfw0PwPUW8KFkZuDkwvbOBK_-UfsBOl2RMg</recordid><startdate>20160513</startdate><enddate>20160513</enddate><creator>Ozaki, Tomohiko</creator><creator>Muramatsu, Rieko</creator><creator>Sasai, Miwa</creator><creator>Yamamoto, Masahiro</creator><creator>Kubota, Yoshiaki</creator><creator>Fujinaka, Toshiyuki</creator><creator>Yoshimine, Toshiki</creator><creator>Yamashita, Toshihide</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160513</creationdate><title>The P2X4 receptor is required for neuroprotection via ischemic preconditioning</title><author>Ozaki, Tomohiko ; Muramatsu, Rieko ; Sasai, Miwa ; Yamamoto, Masahiro ; Kubota, Yoshiaki ; Fujinaka, Toshiyuki ; Yoshimine, Toshiki ; Yamashita, Toshihide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-240537d2006eb760d22e3a5b71594e3e9da12789116e14cbec806ff73b1bf2f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>38/61</topic><topic>42/109</topic><topic>631/378</topic><topic>64/60</topic><topic>692/617</topic><topic>82/51</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozaki, Tomohiko</creatorcontrib><creatorcontrib>Muramatsu, Rieko</creatorcontrib><creatorcontrib>Sasai, Miwa</creatorcontrib><creatorcontrib>Yamamoto, Masahiro</creatorcontrib><creatorcontrib>Kubota, Yoshiaki</creatorcontrib><creatorcontrib>Fujinaka, Toshiyuki</creatorcontrib><creatorcontrib>Yoshimine, Toshiki</creatorcontrib><creatorcontrib>Yamashita, Toshihide</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozaki, Tomohiko</au><au>Muramatsu, Rieko</au><au>Sasai, Miwa</au><au>Yamamoto, Masahiro</au><au>Kubota, Yoshiaki</au><au>Fujinaka, Toshiyuki</au><au>Yoshimine, Toshiki</au><au>Yamashita, Toshihide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The P2X4 receptor is required for neuroprotection via ischemic preconditioning</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-05-13</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>25893</spage><epage>25893</epage><pages>25893-25893</pages><artnum>25893</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Ischemic preconditioning (IPC), a procedure consisting of transient ischemia and subsequent reperfusion, provides ischemic tolerance against prolonged ischemia in the brain. Although the blood flow changes mediated by IPC are primarily perceived by vascular endothelial cells, the role of these cells in ischemic tolerance has not been fully clarified. In this study, we found that the P2X4 receptor, which is abundantly expressed in vascular endothelial cells, is required for ischemic tolerance following middle artery occlusion (MCAO) in mice. Mechanistically, the P2X4 receptor was stimulated by fluid shear stress, which mimics reperfusion, thus promoting the increased expression of osteopontin, a neuroprotective molecule. Furthermore, we found that the intracerebroventricular administration of osteopontin was sufficient to exert a neuroprotective effect mediated by preconditioning-stimulated P2X4 receptor activation. These results demonstrate a novel mechanism whereby vascular endothelial cells are involved in ischemic tolerance.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27173846</pmid><doi>10.1038/srep25893</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2016-05, Vol.6 (1), p.25893-25893, Article 25893 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4865734 |
| source | Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 38/61 42/109 631/378 64/60 692/617 82/51 Humanities and Social Sciences multidisciplinary Science |
| title | The P2X4 receptor is required for neuroprotection via ischemic preconditioning |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T07%3A25%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20P2X4%20receptor%20is%20required%20for%20neuroprotection%20via%20ischemic%20preconditioning&rft.jtitle=Scientific%20reports&rft.au=Ozaki,%20Tomohiko&rft.date=2016-05-13&rft.volume=6&rft.issue=1&rft.spage=25893&rft.epage=25893&rft.pages=25893-25893&rft.artnum=25893&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep25893&rft_dat=%3Cproquest_pubme%3E1789494240%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1789494240&rft_id=info:pmid/27173846&rfr_iscdi=true |