The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to cont...
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| Veröffentlicht in: | Gut pathogens 2016-05, Vol.8 (16), p.16-16, Article 16 |
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| creator | Elder, Jacob R Chiok, Kim Lam Paul, Narayan C Haldorson, Gary Guard, Jean Shah, Devendra H |
| description | Salmonella enterica serovar Enteritidis (S. Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to contribute to Salmonella pathogenesis. The objective of the current study was to determine the contribution of SPI-13 to S. Enteritidis pathogenesis.
We deleted the entire SPI-13 (∆SPI-13) from the genome of S. Enteritidis CDC_2010K_0968 strain isolated from a human patient during the 2010 egg-associated outbreak in the US. The kinetics of infection of the wild-type parent and the ∆SPI-13 were compared in orally challenged day-old chickens and streptomycin pre-treated mice. The degree of intestinal inflammation and the survival of mutant strain within the avian (HD11) and murine (RAW264.7) macrophages were also determined.
The deletion of the SPI-13 resulted in impaired infection kinetics of S. Enteritidis in streptomycin pre-treated mice which was characterized by significantly lower (P |
| doi_str_mv | 10.1186/s13099-016-0098-0 |
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We deleted the entire SPI-13 (∆SPI-13) from the genome of S. Enteritidis CDC_2010K_0968 strain isolated from a human patient during the 2010 egg-associated outbreak in the US. The kinetics of infection of the wild-type parent and the ∆SPI-13 were compared in orally challenged day-old chickens and streptomycin pre-treated mice. The degree of intestinal inflammation and the survival of mutant strain within the avian (HD11) and murine (RAW264.7) macrophages were also determined.
The deletion of the SPI-13 resulted in impaired infection kinetics of S. Enteritidis in streptomycin pre-treated mice which was characterized by significantly lower (P < 0.05) viable counts in the ceca, liver and spleen, impaired ability to induce intestinal inflammation and reduced survival within murine macrophages. Conversely, there were no significant differences in the infection kinetics of ∆SPI-13 in day-old chickens in any of the organs tested and the survival of ∆SPI-13 within chicken macrophages remained unaltered.
The results of this study show that SPI-13 contributes to the pathogenesis of S. Enteritidis in streptomycin pre-treated mice but not in day-old chickens and raises the possibility that SPI-13 may play a role in pathogenesis and the host adaptation/restriction of Salmonella serovars.</description><identifier>ISSN: 1757-4749</identifier><identifier>EISSN: 1757-4749</identifier><identifier>DOI: 10.1186/s13099-016-0098-0</identifier><identifier>PMID: 27141235</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Genetic aspects ; Genotype ; Properties ; Salmonella ; Virulence (Microbiology)</subject><ispartof>Gut pathogens, 2016-05, Vol.8 (16), p.16-16, Article 16</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>Elder et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-7ef32ba370f7ba7bf69817bd07f47b8280cbbeba1a19687b7bcb99dba7e012653</citedby><cites>FETCH-LOGICAL-c563t-7ef32ba370f7ba7bf69817bd07f47b8280cbbeba1a19687b7bcb99dba7e012653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852409/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852409/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27141235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elder, Jacob R</creatorcontrib><creatorcontrib>Chiok, Kim Lam</creatorcontrib><creatorcontrib>Paul, Narayan C</creatorcontrib><creatorcontrib>Haldorson, Gary</creatorcontrib><creatorcontrib>Guard, Jean</creatorcontrib><creatorcontrib>Shah, Devendra H</creatorcontrib><title>The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens</title><title>Gut pathogens</title><addtitle>Gut Pathog</addtitle><description>Salmonella enterica serovar Enteritidis (S. Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to contribute to Salmonella pathogenesis. The objective of the current study was to determine the contribution of SPI-13 to S. Enteritidis pathogenesis.
We deleted the entire SPI-13 (∆SPI-13) from the genome of S. Enteritidis CDC_2010K_0968 strain isolated from a human patient during the 2010 egg-associated outbreak in the US. The kinetics of infection of the wild-type parent and the ∆SPI-13 were compared in orally challenged day-old chickens and streptomycin pre-treated mice. The degree of intestinal inflammation and the survival of mutant strain within the avian (HD11) and murine (RAW264.7) macrophages were also determined.
The deletion of the SPI-13 resulted in impaired infection kinetics of S. Enteritidis in streptomycin pre-treated mice which was characterized by significantly lower (P < 0.05) viable counts in the ceca, liver and spleen, impaired ability to induce intestinal inflammation and reduced survival within murine macrophages. Conversely, there were no significant differences in the infection kinetics of ∆SPI-13 in day-old chickens in any of the organs tested and the survival of ∆SPI-13 within chicken macrophages remained unaltered.
The results of this study show that SPI-13 contributes to the pathogenesis of S. Enteritidis in streptomycin pre-treated mice but not in day-old chickens and raises the possibility that SPI-13 may play a role in pathogenesis and the host adaptation/restriction of Salmonella serovars.</description><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Properties</subject><subject>Salmonella</subject><subject>Virulence (Microbiology)</subject><issn>1757-4749</issn><issn>1757-4749</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkk9v1DAQxSMEoqXwAbggS0iIS4qdP3Z8QaqqFpAqcaCcLduZbFwSO8QO0p746ky0ZdlFqxyScX5vbL95Wfaa0UvGGv4hspJKmVPGc0plk9Mn2TkTtcgrUcmnB99n2YsYHyjlVdXUz7OzQrCKFWV9nv2-74F808MYPAyDJpNOfdiAd9alLXFx0L4lrCQ2-DQ7sySIJIU9BtFF4jyJaYYphXFrsZhmyLHWCVoyOgsEZcSHtIKt3uZhaIntnf0BPr7MnnV6iPDq8X2Rfb-9ub_-nN99_fTl-uoutzUvUy6gKwujS0E7YbQwHZcNE6aloquEaYqGWmPAaKaZ5I0wwlgjZYsoUFbwurzIPu76TosZobWA19GDmmY36nmrgnbq-I93vdqEXwoNKyoqscH7xwZz-LlATGp00a6eeQhLVEw0gjYNzgPRt_-hD2GZPV4PKSnxOIKJf9RGD6Cc7wLua9em6qqqWVlUnHOk8hPU6jweEmfWOVw-4i9P8Pi0gKM4KXh3IOhBD6mPYViSCz4eg2wH2jnEOEO3N49RtcZR7eKoMI5qjaOiqHlz6Ppe8Td_5R8KGdt7</recordid><startdate>20160502</startdate><enddate>20160502</enddate><creator>Elder, Jacob R</creator><creator>Chiok, Kim Lam</creator><creator>Paul, Narayan C</creator><creator>Haldorson, Gary</creator><creator>Guard, Jean</creator><creator>Shah, Devendra H</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160502</creationdate><title>The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens</title><author>Elder, Jacob R ; 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Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to contribute to Salmonella pathogenesis. The objective of the current study was to determine the contribution of SPI-13 to S. Enteritidis pathogenesis.
We deleted the entire SPI-13 (∆SPI-13) from the genome of S. Enteritidis CDC_2010K_0968 strain isolated from a human patient during the 2010 egg-associated outbreak in the US. The kinetics of infection of the wild-type parent and the ∆SPI-13 were compared in orally challenged day-old chickens and streptomycin pre-treated mice. The degree of intestinal inflammation and the survival of mutant strain within the avian (HD11) and murine (RAW264.7) macrophages were also determined.
The deletion of the SPI-13 resulted in impaired infection kinetics of S. Enteritidis in streptomycin pre-treated mice which was characterized by significantly lower (P < 0.05) viable counts in the ceca, liver and spleen, impaired ability to induce intestinal inflammation and reduced survival within murine macrophages. Conversely, there were no significant differences in the infection kinetics of ∆SPI-13 in day-old chickens in any of the organs tested and the survival of ∆SPI-13 within chicken macrophages remained unaltered.
The results of this study show that SPI-13 contributes to the pathogenesis of S. Enteritidis in streptomycin pre-treated mice but not in day-old chickens and raises the possibility that SPI-13 may play a role in pathogenesis and the host adaptation/restriction of Salmonella serovars.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27141235</pmid><doi>10.1186/s13099-016-0098-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Springer Nature OA Free Journals; PubMed Central |
| subjects | Genetic aspects Genotype Properties Salmonella Virulence (Microbiology) |
| title | The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens |
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