Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum
Yiguanjian is administered in traditional Chinese medicine for liver diseases and has been demonstrated to reduce liver fibrosis. This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differen...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2016-01, Vol.2016 (2016), p.1-12 |
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| creator | Leng, Aijing Wang, Ling Zhu, Ying Pang, Bingyao Fu, Linlin Chen, Hai-Long |
| description | Yiguanjian is administered in traditional Chinese medicine for liver diseases and has been demonstrated to reduce liver fibrosis. This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differentiation of murine bone-marrow-derived mesenchymal cells (BM-MSCs) into hepatocytes in vitro. Adherent MSCs were isolated from murine bone marrow. Differentiation was induced by 20 ng/mL HGF, 50 ng/mL SDF-1, and 20% Yiguanjian drug-containing serum for 7 to 28 days, and mature hepatocytes’ marker albumin (ALB) and cholangiocytes’ marker cytokeratin-18 (CK-18) were assessed by immunocytochemistry and western blot. BM-MSCs exhibited homogeneous spindle shape growth after subculture and stained positive for CD90 and negative for CD34. After induction with HGF + normal serum or YGJ for 14 days, HGF + SDF-1 + normal serum for 7 days, or HGF + SDF-1 + YGJ for 5 days, MSCs’ morphology changed gradually and begun to resemble hepatocyte-like cells. Cultures supplemented with HGF + SDF-1 + YGJ contained significantly higher proportions of ALB and CK-18 positive cells than cultures supplemented with HGF + SDF-1 + normal serum at day 7. These observations corroborated the results of western blot. In conclusion, Yiguanjian drug-containing serum could facilitate the differentiation of murine BM-MSCs into hepatocytes in vitro and has a synergistic effect with SDF-1 and HGF. |
| doi_str_mv | 10.1155/2016/7380439 |
| format | Article |
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This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differentiation of murine bone-marrow-derived mesenchymal cells (BM-MSCs) into hepatocytes in vitro. Adherent MSCs were isolated from murine bone marrow. Differentiation was induced by 20 ng/mL HGF, 50 ng/mL SDF-1, and 20% Yiguanjian drug-containing serum for 7 to 28 days, and mature hepatocytes’ marker albumin (ALB) and cholangiocytes’ marker cytokeratin-18 (CK-18) were assessed by immunocytochemistry and western blot. BM-MSCs exhibited homogeneous spindle shape growth after subculture and stained positive for CD90 and negative for CD34. After induction with HGF + normal serum or YGJ for 14 days, HGF + SDF-1 + normal serum for 7 days, or HGF + SDF-1 + YGJ for 5 days, MSCs’ morphology changed gradually and begun to resemble hepatocyte-like cells. Cultures supplemented with HGF + SDF-1 + YGJ contained significantly higher proportions of ALB and CK-18 positive cells than cultures supplemented with HGF + SDF-1 + normal serum at day 7. These observations corroborated the results of western blot. In conclusion, Yiguanjian drug-containing serum could facilitate the differentiation of murine BM-MSCs into hepatocytes in vitro and has a synergistic effect with SDF-1 and HGF.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2016/7380439</identifier><identifier>PMID: 27190538</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Albumin ; Amino acids ; Bone marrow ; Cell differentiation ; Chinese medicine ; Cytokines ; Drug dosages ; Ethylenediaminetetraacetic acid ; Hospitals ; Kinases ; Liver ; Liver diseases ; Medicine, Oriental ; Rodents ; Stem cells ; Transplants & implants</subject><ispartof>Evidence-based complementary and alternative medicine, 2016-01, Vol.2016 (2016), p.1-12</ispartof><rights>Copyright © 2016 Linlin Fu et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 Linlin Fu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 Linlin Fu et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-800c48b93fa9f768dcb3a8875a07bd923860de2dfd0ee2a72cfec4d034986baf3</citedby><cites>FETCH-LOGICAL-c461t-800c48b93fa9f768dcb3a8875a07bd923860de2dfd0ee2a72cfec4d034986baf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27190538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gong, Yuewen</contributor><creatorcontrib>Leng, Aijing</creatorcontrib><creatorcontrib>Wang, Ling</creatorcontrib><creatorcontrib>Zhu, Ying</creatorcontrib><creatorcontrib>Pang, Bingyao</creatorcontrib><creatorcontrib>Fu, Linlin</creatorcontrib><creatorcontrib>Chen, Hai-Long</creatorcontrib><title>Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Yiguanjian is administered in traditional Chinese medicine for liver diseases and has been demonstrated to reduce liver fibrosis. This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differentiation of murine bone-marrow-derived mesenchymal cells (BM-MSCs) into hepatocytes in vitro. Adherent MSCs were isolated from murine bone marrow. Differentiation was induced by 20 ng/mL HGF, 50 ng/mL SDF-1, and 20% Yiguanjian drug-containing serum for 7 to 28 days, and mature hepatocytes’ marker albumin (ALB) and cholangiocytes’ marker cytokeratin-18 (CK-18) were assessed by immunocytochemistry and western blot. BM-MSCs exhibited homogeneous spindle shape growth after subculture and stained positive for CD90 and negative for CD34. After induction with HGF + normal serum or YGJ for 14 days, HGF + SDF-1 + normal serum for 7 days, or HGF + SDF-1 + YGJ for 5 days, MSCs’ morphology changed gradually and begun to resemble hepatocyte-like cells. Cultures supplemented with HGF + SDF-1 + YGJ contained significantly higher proportions of ALB and CK-18 positive cells than cultures supplemented with HGF + SDF-1 + normal serum at day 7. These observations corroborated the results of western blot. In conclusion, Yiguanjian drug-containing serum could facilitate the differentiation of murine BM-MSCs into hepatocytes in vitro and has a synergistic effect with SDF-1 and HGF.</description><subject>Albumin</subject><subject>Amino acids</subject><subject>Bone marrow</subject><subject>Cell differentiation</subject><subject>Chinese medicine</subject><subject>Cytokines</subject><subject>Drug dosages</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Hospitals</subject><subject>Kinases</subject><subject>Liver</subject><subject>Liver diseases</subject><subject>Medicine, Oriental</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Transplants & implants</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNksGO0zAQhiMEYpeFG2dkiQsShLWTNHYuKy1pl11pKw4FBCfLccatq8QuttOl78bD4dDSBU7IB489n_-Z8UySPCf4LSGTyXmGSXlOc4aLvHqQnBJakLTIGHt4tOmXk-SJ92uMs4pS-jg5ySip8CRnp8mP2Xf0WW8tWgRne9GhGrounYLTW2jRlZDBOkTSObRahHgz1UqBAxPiUVuDrEJzO3hA76wBNBfO2Ts0Bw9Grnaj3iJA_0vUI22CRdewEcHKXQCPbjyamZUwMgo3O1SvtIkv0RhMRhN91ctBmLUWBk3dsExra4LQRpslWoAb-qfJIyU6D88O-1ny6Wr2sb5Obz-8v6kvb1NZlCSkDGNZsKbKlagULVkrm1wwRicC06atspyVuIWsVS0GyATNpAJZtDgvKlY2QuVnycVedzM0PbQylu9ExzdO98LtuBWa_-0xesWXdssLFleWRYFXBwFnvw3gA--1l_FThIH4e5zQKjYHT8iIvvwHXdvBmVhepFhMtSgwuaeWogOujbIxrhxF-WVJcE5KxnCk3uwp6az3DtQxZYL5ODx8HB5-GJ6Iv_izzCP8e1oi8HoPxEa14k7_pxxEBpS4p0n0F2X-E5V52GQ</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Leng, Aijing</creator><creator>Wang, Ling</creator><creator>Zhu, Ying</creator><creator>Pang, Bingyao</creator><creator>Fu, Linlin</creator><creator>Chen, Hai-Long</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum</title><author>Leng, Aijing ; 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This study investigated the effect of Yiguanjian drug-containing serum (YGJ) with Stromal Cell-Derived Factor 1 (SDF-1) and Hepatocyte Growth Factor (HGF) on the differentiation of murine bone-marrow-derived mesenchymal cells (BM-MSCs) into hepatocytes in vitro. Adherent MSCs were isolated from murine bone marrow. Differentiation was induced by 20 ng/mL HGF, 50 ng/mL SDF-1, and 20% Yiguanjian drug-containing serum for 7 to 28 days, and mature hepatocytes’ marker albumin (ALB) and cholangiocytes’ marker cytokeratin-18 (CK-18) were assessed by immunocytochemistry and western blot. BM-MSCs exhibited homogeneous spindle shape growth after subculture and stained positive for CD90 and negative for CD34. After induction with HGF + normal serum or YGJ for 14 days, HGF + SDF-1 + normal serum for 7 days, or HGF + SDF-1 + YGJ for 5 days, MSCs’ morphology changed gradually and begun to resemble hepatocyte-like cells. Cultures supplemented with HGF + SDF-1 + YGJ contained significantly higher proportions of ALB and CK-18 positive cells than cultures supplemented with HGF + SDF-1 + normal serum at day 7. These observations corroborated the results of western blot. In conclusion, Yiguanjian drug-containing serum could facilitate the differentiation of murine BM-MSCs into hepatocytes in vitro and has a synergistic effect with SDF-1 and HGF.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>27190538</pmid><doi>10.1155/2016/7380439</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Albumin Amino acids Bone marrow Cell differentiation Chinese medicine Cytokines Drug dosages Ethylenediaminetetraacetic acid Hospitals Kinases Liver Liver diseases Medicine, Oriental Rodents Stem cells Transplants & implants |
| title | Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum |
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