Quantitative methylation analysis reveals distinct association between PAX6 methylation and clinical characteristics with different viral infections in hepatocellular carcinoma
Related to genetic alteration, frequent promoter hypermethylation is also a contributing factor in the development of human cancers. Recently, we discovered numerous novel genes that were aberrantly methylated in hepatocellular carcinoma (HCC) by using Infinium HumanMethylation27 BeadChip array. We...
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| Veröffentlicht in: | Clinical epigenetics 2016-04, Vol.8 (41), p.41-41, Article 41 |
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| container_title | Clinical epigenetics |
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| creator | Shih, Yu-Lueng Kuo, Chih-Chi Yan, Ming-De Lin, Ya-Wen Hsieh, Chung-Bao Hsieh, Tsai-Yuan |
| description | Related to genetic alteration, frequent promoter hypermethylation is also a contributing factor in the development of human cancers. Recently, we discovered numerous novel genes that were aberrantly methylated in hepatocellular carcinoma (HCC) by using Infinium HumanMethylation27 BeadChip array. We utilized a quantitative methylation-specific PCR (Q-MSP) system for the evaluation of PAX6 methylation in 29 normal controls and 160 paired HCC tissues and their adjacent non-tumor tissues. We verified the correlation between the methylation status of PAX6 and clinical characteristics with different viral status.
Paired-box 6 promoter methylation was observed in 39.4 %, 15.6 %, and 3.4 % in primary HCCs, adjacent non-tumors, and normal control tissues, respectively. Methylation of the PAX6 promoter region in HCCs significantly increased compared with control tissues. PAX6 was frequently methylated in HCV-positive HCC tissues (61.3 %) and rarely methylated in HBV-positive (22.1 %) and double-negative HCC tissues (33.3 %).
Our data suggests that promoter hypermethylation of PAX6 is a common event in HCCs and the association of PAX6 methylation in clinicopathological features is divergent with different viral status. |
| doi_str_mv | 10.1186/s13148-016-0208-3 |
| format | Article |
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Paired-box 6 promoter methylation was observed in 39.4 %, 15.6 %, and 3.4 % in primary HCCs, adjacent non-tumors, and normal control tissues, respectively. Methylation of the PAX6 promoter region in HCCs significantly increased compared with control tissues. PAX6 was frequently methylated in HCV-positive HCC tissues (61.3 %) and rarely methylated in HBV-positive (22.1 %) and double-negative HCC tissues (33.3 %).
Our data suggests that promoter hypermethylation of PAX6 is a common event in HCCs and the association of PAX6 methylation in clinicopathological features is divergent with different viral status.</description><identifier>ISSN: 1868-7075</identifier><identifier>ISSN: 1868-7083</identifier><identifier>EISSN: 1868-7083</identifier><identifier>EISSN: 1868-7075</identifier><identifier>DOI: 10.1186/s13148-016-0208-3</identifier><identifier>PMID: 27110298</identifier><language>eng</language><publisher>Germany: BioMed Central Ltd</publisher><subject>Aged ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Carcinoma, Hepatocellular - virology ; Cell Line, Tumor ; DNA Methylation ; Female ; Gene Expression Regulation, Neoplastic ; Health aspects ; Hepatitis B - genetics ; Hepatitis C - genetics ; Hepatitis C virus ; Hepatoma ; Humans ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Liver Neoplasms - virology ; Male ; Methylation ; Middle Aged ; PAX6 Transcription Factor - genetics ; Prognosis ; Promoter Regions, Genetic ; Risk factors</subject><ispartof>Clinical epigenetics, 2016-04, Vol.8 (41), p.41-41, Article 41</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>Shih et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-c89ce87bd507c976f9ffa02f6dc42951172dd068eaf063f714486103ad367fb03</citedby><cites>FETCH-LOGICAL-c494t-c89ce87bd507c976f9ffa02f6dc42951172dd068eaf063f714486103ad367fb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841049/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841049/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27110298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shih, Yu-Lueng</creatorcontrib><creatorcontrib>Kuo, Chih-Chi</creatorcontrib><creatorcontrib>Yan, Ming-De</creatorcontrib><creatorcontrib>Lin, Ya-Wen</creatorcontrib><creatorcontrib>Hsieh, Chung-Bao</creatorcontrib><creatorcontrib>Hsieh, Tsai-Yuan</creatorcontrib><title>Quantitative methylation analysis reveals distinct association between PAX6 methylation and clinical characteristics with different viral infections in hepatocellular carcinoma</title><title>Clinical epigenetics</title><addtitle>Clin Epigenetics</addtitle><description>Related to genetic alteration, frequent promoter hypermethylation is also a contributing factor in the development of human cancers. Recently, we discovered numerous novel genes that were aberrantly methylated in hepatocellular carcinoma (HCC) by using Infinium HumanMethylation27 BeadChip array. We utilized a quantitative methylation-specific PCR (Q-MSP) system for the evaluation of PAX6 methylation in 29 normal controls and 160 paired HCC tissues and their adjacent non-tumor tissues. We verified the correlation between the methylation status of PAX6 and clinical characteristics with different viral status.
Paired-box 6 promoter methylation was observed in 39.4 %, 15.6 %, and 3.4 % in primary HCCs, adjacent non-tumors, and normal control tissues, respectively. Methylation of the PAX6 promoter region in HCCs significantly increased compared with control tissues. PAX6 was frequently methylated in HCV-positive HCC tissues (61.3 %) and rarely methylated in HBV-positive (22.1 %) and double-negative HCC tissues (33.3 %).
Our data suggests that promoter hypermethylation of PAX6 is a common event in HCCs and the association of PAX6 methylation in clinicopathological features is divergent with different viral status.</description><subject>Aged</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Cell Line, Tumor</subject><subject>DNA Methylation</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Health aspects</subject><subject>Hepatitis B - genetics</subject><subject>Hepatitis C - genetics</subject><subject>Hepatitis C virus</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - virology</subject><subject>Male</subject><subject>Methylation</subject><subject>Middle Aged</subject><subject>PAX6 Transcription Factor - genetics</subject><subject>Prognosis</subject><subject>Promoter Regions, Genetic</subject><subject>Risk factors</subject><issn>1868-7075</issn><issn>1868-7083</issn><issn>1868-7083</issn><issn>1868-7075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptUl1rFDEUHUSxpfYH-CIBX3yZmkwy-XgRllI_oKCCgm8hm7nppswka5LZsv_Kn2iGrastJg-53Jxz7genaV4SfEGI5G8zoYTJFhPe4g7Llj5pTmtetgJL-vQYi_6kOc_5FtdDlVIEP29OOkEI7pQ8bX59nU0ovpjid4AmKJv9WOMYkAlm3GefUYIdmDGjwefigy3I5BytP6DWUO4AAvqy-sEf0QdkRx-8NSOyG5OMLZAWCZvRnS-bquccJAgF7XyqIB8c2IWba4g2sDUlWhjHeTQJWZOsD3EyL5pnrnYD5_fvWfP9_dW3y4_t9ecPny5X161lipXWSmVBivXQY2GV4E45Z3Dn-GBZp3pCRDcMmEswDnPqBGFMcoKpGSgXbo3pWfPuoLud1xMMtvZZm9Tb5CeT9joarx_-BL_RN3GnmWQEM1UF3twLpPhzhlz05PMyjwkQ56yJkIzxWrev0NePoLdxTnX_C0pxynF9_qJuzAi6bivWunYR1SvWE0p5T1lFXfwHVe8Ak7cxgPM1_4BADgSbYs4J3HFGgvXiNH1wmq5O04vTNK2cV_8u58j44yv6Gyct0yc</recordid><startdate>20160422</startdate><enddate>20160422</enddate><creator>Shih, Yu-Lueng</creator><creator>Kuo, Chih-Chi</creator><creator>Yan, Ming-De</creator><creator>Lin, Ya-Wen</creator><creator>Hsieh, Chung-Bao</creator><creator>Hsieh, Tsai-Yuan</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160422</creationdate><title>Quantitative methylation analysis reveals distinct association between PAX6 methylation and clinical characteristics with different viral infections in hepatocellular carcinoma</title><author>Shih, Yu-Lueng ; Kuo, Chih-Chi ; Yan, Ming-De ; Lin, Ya-Wen ; Hsieh, Chung-Bao ; Hsieh, Tsai-Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-c89ce87bd507c976f9ffa02f6dc42951172dd068eaf063f714486103ad367fb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aged</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Carcinoma, Hepatocellular - virology</topic><topic>Cell Line, Tumor</topic><topic>DNA Methylation</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Health aspects</topic><topic>Hepatitis B - genetics</topic><topic>Hepatitis C - genetics</topic><topic>Hepatitis C virus</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - virology</topic><topic>Male</topic><topic>Methylation</topic><topic>Middle Aged</topic><topic>PAX6 Transcription Factor - genetics</topic><topic>Prognosis</topic><topic>Promoter Regions, Genetic</topic><topic>Risk factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shih, Yu-Lueng</creatorcontrib><creatorcontrib>Kuo, Chih-Chi</creatorcontrib><creatorcontrib>Yan, Ming-De</creatorcontrib><creatorcontrib>Lin, Ya-Wen</creatorcontrib><creatorcontrib>Hsieh, Chung-Bao</creatorcontrib><creatorcontrib>Hsieh, Tsai-Yuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical epigenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shih, Yu-Lueng</au><au>Kuo, Chih-Chi</au><au>Yan, Ming-De</au><au>Lin, Ya-Wen</au><au>Hsieh, Chung-Bao</au><au>Hsieh, Tsai-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative methylation analysis reveals distinct association between PAX6 methylation and clinical characteristics with different viral infections in hepatocellular carcinoma</atitle><jtitle>Clinical epigenetics</jtitle><addtitle>Clin Epigenetics</addtitle><date>2016-04-22</date><risdate>2016</risdate><volume>8</volume><issue>41</issue><spage>41</spage><epage>41</epage><pages>41-41</pages><artnum>41</artnum><issn>1868-7075</issn><issn>1868-7083</issn><eissn>1868-7083</eissn><eissn>1868-7075</eissn><abstract>Related to genetic alteration, frequent promoter hypermethylation is also a contributing factor in the development of human cancers. Recently, we discovered numerous novel genes that were aberrantly methylated in hepatocellular carcinoma (HCC) by using Infinium HumanMethylation27 BeadChip array. We utilized a quantitative methylation-specific PCR (Q-MSP) system for the evaluation of PAX6 methylation in 29 normal controls and 160 paired HCC tissues and their adjacent non-tumor tissues. We verified the correlation between the methylation status of PAX6 and clinical characteristics with different viral status.
Paired-box 6 promoter methylation was observed in 39.4 %, 15.6 %, and 3.4 % in primary HCCs, adjacent non-tumors, and normal control tissues, respectively. Methylation of the PAX6 promoter region in HCCs significantly increased compared with control tissues. PAX6 was frequently methylated in HCV-positive HCC tissues (61.3 %) and rarely methylated in HBV-positive (22.1 %) and double-negative HCC tissues (33.3 %).
Our data suggests that promoter hypermethylation of PAX6 is a common event in HCCs and the association of PAX6 methylation in clinicopathological features is divergent with different viral status.</abstract><cop>Germany</cop><pub>BioMed Central Ltd</pub><pmid>27110298</pmid><doi>10.1186/s13148-016-0208-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - virology Cell Line, Tumor DNA Methylation Female Gene Expression Regulation, Neoplastic Health aspects Hepatitis B - genetics Hepatitis C - genetics Hepatitis C virus Hepatoma Humans Liver Neoplasms - genetics Liver Neoplasms - pathology Liver Neoplasms - virology Male Methylation Middle Aged PAX6 Transcription Factor - genetics Prognosis Promoter Regions, Genetic Risk factors |
| title | Quantitative methylation analysis reveals distinct association between PAX6 methylation and clinical characteristics with different viral infections in hepatocellular carcinoma |
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