Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite

Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite

Exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. Silymarin with an antioxidant property scavenges free radicals. The aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma m...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of reproductive biomedicine (Yazd, Iran) Iran), 2016-01, Vol.14 (1), p.47-52
Hauptverfasser: Eskandari, Farzaneh, Momeni, Hamid Reza
Format: Artikel
Sprache:eng
Schlagworte:
Original
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 52
container_issue 1
container_start_page 47
container_title International journal of reproductive biomedicine (Yazd, Iran)
container_volume 14
creator Eskandari, Farzaneh
Momeni, Hamid Reza
description Exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. Silymarin with an antioxidant property scavenges free radicals. The aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. Ram epidydimal spermatozoa were divided into five groups: spermatozoa at 0 hr, spermatozoa at 180 min (control), spermatozoa treated with silymarin (20 μM) + sodium arsenite (10 μM) for 180 min, spermatozoa treated with sodium arsenite (10 μM) for 180 min and spermatozoa treated with silymarin (20 μM) for 180 min. Double staining of Hoechst and propidium iodide was performed to evaluate sperm plasma membrane integrity, whereas comassie brilliant blue staining was used to assess acrosome integrity. Plasma membrane (p< 0.001) and acrosome integrity (p< 0.05) of the spermatozoa were significantly reduced in sodium arsenite group compared to the control. In silymarin + sodium arsenite group, silymarin was able to significantly (p< 0.001) ameliorate the adverse effects of sodium arsenite on these sperm parameters compared to sodium arsenite group. The incubation of sperm for 180 min (control group) showed a significant (p< 0.001) decrease in acrosome integrity compared to the spermatozoa at 0 hour. The application of silymarin alone for 180 min could also significantly (p< 0.05) increase sperm acrosome integrity compared to the control. Silymarin as a potent antioxidant could compensate the adverse effects of sodium arsenite on the ram sperm plasma membrane and acrosome integrity.
doi_str_mv 10.29252/ijrm.14.1.47
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4837919</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4156616941</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-8602aa45c100de5d91a7c9a96135da38bdb1dc077370b57b5ba8fa2d7e06269e3</originalsourceid><addsrcrecordid>eNpdkcFrFDEUxoNYbNn26FUCXrzMmpdkJpmLIMWqUPCgnkNm8rbNMpmMSUbZ_75Zuxb19B68Hx_f9z5CXgLb8p63_K3fp7AFuYWtVM_IBZeqa4RS_Plpl8D0ObnKec8Yg64F4OIFOecKJLRSXxDz1U-HYJOf6ZJiwbFkukw2B0sDhiHZGamdHbVjijkGpH4ueJd8OdSN5gVToCWhLejoL1_uaY7Or4HalHH2BS_J2c5OGa9Oc0O-33z4dv2puf3y8fP1-9tmrEZKozvGrZXtCIw5bF0PVo297TsQrbNCD24ANzKlhGJDq4Z2sHpnuVPIOt71KDbk3aPusg4B3YhzSXYyS_I13MFE682_l9nfm7v400gtVA99FXhzEkjxx4q5mODziNNUPxDXbEBpxbTmoqvo6__QfVzTXOMZ0NWw0EffG9I8UsfP5YS7JzPAzO_2zLE9A9KAkaryr_5O8ET_6Uo8ABiUmDc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1813538961</pqid></control><display><type>article</type><title>Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><creator>Eskandari, Farzaneh ; Momeni, Hamid Reza</creator><creatorcontrib>Eskandari, Farzaneh ; Momeni, Hamid Reza ; Department of Biology, Faculty of Science, Arak University, Arak, Iran</creatorcontrib><description>Exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. Silymarin with an antioxidant property scavenges free radicals. The aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. Ram epidydimal spermatozoa were divided into five groups: spermatozoa at 0 hr, spermatozoa at 180 min (control), spermatozoa treated with silymarin (20 μM) + sodium arsenite (10 μM) for 180 min, spermatozoa treated with sodium arsenite (10 μM) for 180 min and spermatozoa treated with silymarin (20 μM) for 180 min. Double staining of Hoechst and propidium iodide was performed to evaluate sperm plasma membrane integrity, whereas comassie brilliant blue staining was used to assess acrosome integrity. Plasma membrane (p&lt; 0.001) and acrosome integrity (p&lt; 0.05) of the spermatozoa were significantly reduced in sodium arsenite group compared to the control. In silymarin + sodium arsenite group, silymarin was able to significantly (p&lt; 0.001) ameliorate the adverse effects of sodium arsenite on these sperm parameters compared to sodium arsenite group. The incubation of sperm for 180 min (control group) showed a significant (p&lt; 0.001) decrease in acrosome integrity compared to the spermatozoa at 0 hour. The application of silymarin alone for 180 min could also significantly (p&lt; 0.05) increase sperm acrosome integrity compared to the control. Silymarin as a potent antioxidant could compensate the adverse effects of sodium arsenite on the ram sperm plasma membrane and acrosome integrity.</description><identifier>ISSN: 2476-4108</identifier><identifier>EISSN: 2476-3772</identifier><identifier>DOI: 10.29252/ijrm.14.1.47</identifier><identifier>PMID: 27141548</identifier><language>eng</language><publisher>Iran: Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</publisher><subject>Original</subject><ispartof>International journal of reproductive biomedicine (Yazd, Iran), 2016-01, Vol.14 (1), p.47-52</ispartof><rights>Copyright Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility Jan 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-8602aa45c100de5d91a7c9a96135da38bdb1dc077370b57b5ba8fa2d7e06269e3</citedby><cites>FETCH-LOGICAL-c415t-8602aa45c100de5d91a7c9a96135da38bdb1dc077370b57b5ba8fa2d7e06269e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837919/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837919/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27141548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eskandari, Farzaneh</creatorcontrib><creatorcontrib>Momeni, Hamid Reza</creatorcontrib><creatorcontrib>Department of Biology, Faculty of Science, Arak University, Arak, Iran</creatorcontrib><title>Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite</title><title>International journal of reproductive biomedicine (Yazd, Iran)</title><addtitle>Int J Reprod Biomed (Yazd)</addtitle><description>Exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. Silymarin with an antioxidant property scavenges free radicals. The aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. Ram epidydimal spermatozoa were divided into five groups: spermatozoa at 0 hr, spermatozoa at 180 min (control), spermatozoa treated with silymarin (20 μM) + sodium arsenite (10 μM) for 180 min, spermatozoa treated with sodium arsenite (10 μM) for 180 min and spermatozoa treated with silymarin (20 μM) for 180 min. Double staining of Hoechst and propidium iodide was performed to evaluate sperm plasma membrane integrity, whereas comassie brilliant blue staining was used to assess acrosome integrity. Plasma membrane (p&lt; 0.001) and acrosome integrity (p&lt; 0.05) of the spermatozoa were significantly reduced in sodium arsenite group compared to the control. In silymarin + sodium arsenite group, silymarin was able to significantly (p&lt; 0.001) ameliorate the adverse effects of sodium arsenite on these sperm parameters compared to sodium arsenite group. The incubation of sperm for 180 min (control group) showed a significant (p&lt; 0.001) decrease in acrosome integrity compared to the spermatozoa at 0 hour. The application of silymarin alone for 180 min could also significantly (p&lt; 0.05) increase sperm acrosome integrity compared to the control. Silymarin as a potent antioxidant could compensate the adverse effects of sodium arsenite on the ram sperm plasma membrane and acrosome integrity.</description><subject>Original</subject><issn>2476-4108</issn><issn>2476-3772</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkcFrFDEUxoNYbNn26FUCXrzMmpdkJpmLIMWqUPCgnkNm8rbNMpmMSUbZ_75Zuxb19B68Hx_f9z5CXgLb8p63_K3fp7AFuYWtVM_IBZeqa4RS_Plpl8D0ObnKec8Yg64F4OIFOecKJLRSXxDz1U-HYJOf6ZJiwbFkukw2B0sDhiHZGamdHbVjijkGpH4ueJd8OdSN5gVToCWhLejoL1_uaY7Or4HalHH2BS_J2c5OGa9Oc0O-33z4dv2puf3y8fP1-9tmrEZKozvGrZXtCIw5bF0PVo297TsQrbNCD24ANzKlhGJDq4Z2sHpnuVPIOt71KDbk3aPusg4B3YhzSXYyS_I13MFE682_l9nfm7v400gtVA99FXhzEkjxx4q5mODziNNUPxDXbEBpxbTmoqvo6__QfVzTXOMZ0NWw0EffG9I8UsfP5YS7JzPAzO_2zLE9A9KAkaryr_5O8ET_6Uo8ABiUmDc</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Eskandari, Farzaneh</creator><creator>Momeni, Hamid Reza</creator><general>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</general><general>Research and Clinical Center for Infertility</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201601</creationdate><title>Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite</title><author>Eskandari, Farzaneh ; Momeni, Hamid Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-8602aa45c100de5d91a7c9a96135da38bdb1dc077370b57b5ba8fa2d7e06269e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eskandari, Farzaneh</creatorcontrib><creatorcontrib>Momeni, Hamid Reza</creatorcontrib><creatorcontrib>Department of Biology, Faculty of Science, Arak University, Arak, Iran</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Middle East &amp; Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of reproductive biomedicine (Yazd, Iran)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eskandari, Farzaneh</au><au>Momeni, Hamid Reza</au><aucorp>Department of Biology, Faculty of Science, Arak University, Arak, Iran</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite</atitle><jtitle>International journal of reproductive biomedicine (Yazd, Iran)</jtitle><addtitle>Int J Reprod Biomed (Yazd)</addtitle><date>2016-01</date><risdate>2016</risdate><volume>14</volume><issue>1</issue><spage>47</spage><epage>52</epage><pages>47-52</pages><issn>2476-4108</issn><eissn>2476-3772</eissn><abstract>Exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. Silymarin with an antioxidant property scavenges free radicals. The aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. Ram epidydimal spermatozoa were divided into five groups: spermatozoa at 0 hr, spermatozoa at 180 min (control), spermatozoa treated with silymarin (20 μM) + sodium arsenite (10 μM) for 180 min, spermatozoa treated with sodium arsenite (10 μM) for 180 min and spermatozoa treated with silymarin (20 μM) for 180 min. Double staining of Hoechst and propidium iodide was performed to evaluate sperm plasma membrane integrity, whereas comassie brilliant blue staining was used to assess acrosome integrity. Plasma membrane (p&lt; 0.001) and acrosome integrity (p&lt; 0.05) of the spermatozoa were significantly reduced in sodium arsenite group compared to the control. In silymarin + sodium arsenite group, silymarin was able to significantly (p&lt; 0.001) ameliorate the adverse effects of sodium arsenite on these sperm parameters compared to sodium arsenite group. The incubation of sperm for 180 min (control group) showed a significant (p&lt; 0.001) decrease in acrosome integrity compared to the spermatozoa at 0 hour. The application of silymarin alone for 180 min could also significantly (p&lt; 0.05) increase sperm acrosome integrity compared to the control. Silymarin as a potent antioxidant could compensate the adverse effects of sodium arsenite on the ram sperm plasma membrane and acrosome integrity.</abstract><cop>Iran</cop><pub>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</pub><pmid>27141548</pmid><doi>10.29252/ijrm.14.1.47</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2476-4108
ispartof International journal of reproductive biomedicine (Yazd, Iran), 2016-01, Vol.14 (1), p.47-52
issn 2476-4108
2476-3772
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4837919
source DOAJ Directory of Open Access Journals; PubMed Central
subjects Original
title Silymarin protects plasma membrane and acrosome integrity in sperm treated with sodium arsenite
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T23%3A27%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Silymarin%20protects%20plasma%20membrane%20and%20acrosome%20integrity%20in%20sperm%20treated%20with%20sodium%20arsenite&rft.jtitle=International%20journal%20of%20reproductive%20biomedicine%20(Yazd,%20Iran)&rft.au=Eskandari,%20Farzaneh&rft.aucorp=Department%20of%20Biology,%20Faculty%20of%20Science,%20Arak%20University,%20Arak,%20Iran&rft.date=2016-01&rft.volume=14&rft.issue=1&rft.spage=47&rft.epage=52&rft.pages=47-52&rft.issn=2476-4108&rft.eissn=2476-3772&rft_id=info:doi/10.29252/ijrm.14.1.47&rft_dat=%3Cproquest_pubme%3E4156616941%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1813538961&rft_id=info:pmid/27141548&rfr_iscdi=true