Estimates of Soil Bacterial Ribosome Content and Diversity Are Significantly Affected by the Nucleic Acid Extraction Method Employed

Modern sequencing technologies allow high-resolution analyses of total and potentially active soil microbial communities based on their DNA and RNA, respectively. In the present study, quantitative PCR and 454 pyrosequencing were used to evaluate the effects of different extraction methods on the ab...

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Veröffentlicht in:	Applied and environmental microbiology 2016-05, Vol.82 (9), p.2595-2607
Hauptverfasser:	Wüst, Pia K, Nacke, Heiko, Kaiser, Kristin, Marhan, Sven, Sikorski, Johannes, Kandeler, Ellen, Daniel, Rolf, Overmann, Jörg
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacteria Bacteria Bacteria - chemistry Bacteria - genetics Bacteriological Techniques - methods Biodiversity Cells Deoxyribonucleic acid DNA DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Genes Methods Nucleic Acids - chemistry Nucleic Acids - genetics Nucleic Acids - isolation & purification Phylogeny Polymerase chain reaction Proteobacteria Real-Time Polymerase Chain Reaction - methods Ribonucleic acid Ribosomes - chemistry Ribosomes - genetics RNA RNA, Bacterial - genetics RNA, Bacterial - isolation & purification RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 16S - isolation & purification Sequence Analysis, DNA Soil - chemistry Soil Microbiology
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