Highly Efficient CRISPR/Cas9-Mediated Cloning and Functional Characterization of Gastric Cancer-Derived Epstein-Barr Virus Strains

The Epstein-Barr virus (EBV) is etiologically linked to approximately 10% of gastric cancers, in which viral genomes are maintained as multicopy episomes. EBV-positive gastric cancer cells are incompetent for progeny virus production, making viral DNA cloning extremely difficult. Here we describe a...

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Veröffentlicht in:	Journal of virology 2016-05, Vol.90 (9), p.4383-4393
Hauptverfasser:	Kanda, Teru, Furuse, Yuki, Oshitani, Hitoshi, Kiyono, Tohru
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cell Line, Tumor Chromosomes, Artificial, Bacterial Cloning, Molecular Computational Biology - methods CRISPR-Cas Systems - genetics Cytopathogenic Effect, Viral DNA, Viral Epstein-Barr virus Epstein-Barr Virus Infections - complications Epstein-Barr Virus Infections - virology Gene Targeting - methods Genome, Viral Herpesvirus 4, Human - genetics Humans Molecular Sequence Annotation Molecular Sequence Data Phylogeny Plasmids - genetics ras Proteins - genetics ras Proteins - metabolism Sequence Alignment Sequence Analysis, DNA Spotlight Stomach Neoplasms - etiology Transformation and Oncogenesis Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism Virus Replication
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