Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation
Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differ...
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| Veröffentlicht in: | Scientific reports 2016-04, Vol.6 (1), p.24474-24474, Article 24474 |
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| description | Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering. |
| doi_str_mv | 10.1038/srep24474 |
| format | Article |
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However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep24474</identifier><identifier>PMID: 27091175</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/107 ; 13/51 ; 14 ; 14/19 ; 14/35 ; 14/63 ; 631/61/2049/974 ; 631/61/54/994 ; Alginates - chemistry ; Alginic acid ; Bioprinting ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival ; Citric acid ; Collagen ; Collagen - chemistry ; Cornea ; Cytokeratin ; Epithelial cells ; Epithelium, Corneal - cytology ; Epithelium, Corneal - drug effects ; Gelatin ; Gelatin - chemistry ; Glucuronic Acid - chemistry ; Hexuronic Acids - chemistry ; Humanities and Social Sciences ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry ; Hydrogels ; multidisciplinary ; Printing ; Printing, Three-Dimensional ; Science ; Science (multidisciplinary) ; Sodium ; Sodium alginate ; Sodium citrate ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry</subject><ispartof>Scientific reports, 2016-04, Vol.6 (1), p.24474-24474, Article 24474</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Apr 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a0f08cbdc1a113345f681a3b5c8948145ad062ff9035cfcaddcd1a21a4d193af3</citedby><cites>FETCH-LOGICAL-c438t-a0f08cbdc1a113345f681a3b5c8948145ad062ff9035cfcaddcd1a21a4d193af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835808/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835808/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27091175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Zhengjie</creatorcontrib><creatorcontrib>Su, Xin</creatorcontrib><creatorcontrib>Xu, Yuanyuan</creatorcontrib><creatorcontrib>Kong, Bin</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Mi, Shengli</creatorcontrib><title>Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering.</description><subject>13/107</subject><subject>13/51</subject><subject>14</subject><subject>14/19</subject><subject>14/35</subject><subject>14/63</subject><subject>631/61/2049/974</subject><subject>631/61/54/994</subject><subject>Alginates - chemistry</subject><subject>Alginic acid</subject><subject>Bioprinting</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival</subject><subject>Citric acid</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Cornea</subject><subject>Cytokeratin</subject><subject>Epithelial cells</subject><subject>Epithelium, Corneal - cytology</subject><subject>Epithelium, Corneal - drug effects</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>Glucuronic Acid - chemistry</subject><subject>Hexuronic Acids - chemistry</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</subject><subject>Hydrogels</subject><subject>multidisciplinary</subject><subject>Printing</subject><subject>Printing, Three-Dimensional</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sodium</subject><subject>Sodium alginate</subject><subject>Sodium citrate</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUtrGzEUhUVpqIPrRf9AGcimCUyj51izCbQmLzBkk0J34lrS2AryyJE0Kfn3kbFrnFabK3E_HZ2rg9AXgr8TzORlinZDOZ_yD-iUYi5qyij9eLQfoUlKT7gsQVtO2k9oRKe4JWQqTtHvny5souuz65dVXkVra-PWtk8u9OArbb2vPRjbV9mlNNhKhz7lOOicqj8ur7bnHIP3sPC2MnYZwUAulz-jkw58spN9HaNfN9ePs7t6_nB7P_sxrzVnMteAOyz1wmgChDDGRddIAmwhtGy5JFyAwQ3tuhYzoTsNxmhDgBLghrQMOjZGVzvdzbBYW6NtsQNelZnWEF9VAKfed3q3UsvworhkQmJZBL7tBWJ4HmzKau3Sdm7obRiSIlNJJcOyFQU9-wd9CkMs_1Qo2cpG0qYhhTrfUTqGVNLpDmYIVtvI1CGywn49dn8g_wZUgIsdkLYpLW08evI_tTfufKKy</recordid><startdate>20160419</startdate><enddate>20160419</enddate><creator>Wu, Zhengjie</creator><creator>Su, Xin</creator><creator>Xu, Yuanyuan</creator><creator>Kong, Bin</creator><creator>Sun, Wei</creator><creator>Mi, Shengli</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160419</creationdate><title>Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation</title><author>Wu, Zhengjie ; Su, Xin ; Xu, Yuanyuan ; Kong, Bin ; Sun, Wei ; Mi, Shengli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-a0f08cbdc1a113345f681a3b5c8948145ad062ff9035cfcaddcd1a21a4d193af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13/107</topic><topic>13/51</topic><topic>14</topic><topic>14/19</topic><topic>14/35</topic><topic>14/63</topic><topic>631/61/2049/974</topic><topic>631/61/54/994</topic><topic>Alginates - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Zhengjie</au><au>Su, Xin</au><au>Xu, Yuanyuan</au><au>Kong, Bin</au><au>Sun, Wei</au><au>Mi, Shengli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-04-19</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>24474</spage><epage>24474</epage><pages>24474-24474</pages><artnum>24474</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27091175</pmid><doi>10.1038/srep24474</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/107 13/51 14 14/19 14/35 14/63 631/61/2049/974 631/61/54/994 Alginates - chemistry Alginic acid Bioprinting Cell proliferation Cell Proliferation - drug effects Cell Survival Citric acid Collagen Collagen - chemistry Cornea Cytokeratin Epithelial cells Epithelium, Corneal - cytology Epithelium, Corneal - drug effects Gelatin Gelatin - chemistry Glucuronic Acid - chemistry Hexuronic Acids - chemistry Humanities and Social Sciences Humans Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Hydrogels multidisciplinary Printing Printing, Three-Dimensional Science Science (multidisciplinary) Sodium Sodium alginate Sodium citrate Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry |
| title | Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation |
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