Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control
To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffei...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2016-01, Vol.2016 (2016), p.1-9 |
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| creator | Wang, Yuanjun Fu, Chongluo Li, Aifeng Wang, Yuehua Xuan, Hongzhuan Peng, Wenjun |
| description | To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist “bioactive components,” which also provides a new idea for Chinese propolis quality control. |
| doi_str_mv | 10.1155/2016/9641965 |
| format | Article |
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Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist “bioactive components,” which also provides a new idea for Chinese propolis quality control.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2016/9641965</identifier><identifier>PMID: 27123037</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Amino acids ; Antioxidants ; Apoptosis ; Breast cancer ; Cell growth ; Ethanol ; Flavonoids ; High performance liquid chromatography ; Laboratories ; Polyphenols ; Quality control ; Quality management ; Studies</subject><ispartof>Evidence-based complementary and alternative medicine, 2016-01, Vol.2016 (2016), p.1-9</ispartof><rights>Copyright © 2016 Hongzhuan Xuan et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 Hongzhuan Xuan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 Hongzhuan Xuan et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-171d78674da8b5dde924000c5d9be5d4eb826d414089b0deac8a4d9282b3a1fb3</citedby><cites>FETCH-LOGICAL-c499t-171d78674da8b5dde924000c5d9be5d4eb826d414089b0deac8a4d9282b3a1fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829702/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829702/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27123037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Savka, Michael A.</contributor><creatorcontrib>Wang, Yuanjun</creatorcontrib><creatorcontrib>Fu, Chongluo</creatorcontrib><creatorcontrib>Li, Aifeng</creatorcontrib><creatorcontrib>Wang, Yuehua</creatorcontrib><creatorcontrib>Xuan, Hongzhuan</creatorcontrib><creatorcontrib>Peng, Wenjun</creatorcontrib><title>Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist “bioactive components,” which also provides a new idea for Chinese propolis quality control.</description><subject>Amino acids</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Cell growth</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>High performance liquid chromatography</subject><subject>Laboratories</subject><subject>Polyphenols</subject><subject>Quality control</subject><subject>Quality management</subject><subject>Studies</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkU1rFTEUhoMo9kN3riXgRtBrczKZSbIpXIf6AQUVFN3FzCTTpswk1yTT2n9vhnu9VVeuciAPD-c9L0JPgLwCqOsTSqA5kQ0D2dT30CFwBitGhbi_n_m3A3SU0hUhVHLOH6IDyoFWpOKH6PtrF3Sf3bXFbZg2wVufEw4Dbi-dt8nijzFswugS_qqzjfjsZ46Fx8Hjtc8uz1OIeL0IXL7F2hv8adbjMrfB5xjGR-jBoMdkH-_eY_Tlzdnn9t3q_MPb9-36fNUzKfMKOBguGs6MFl1tjJWUEUL62sjO1obZTtDGMGBEyI4Yq3uhmZFU0K7SMHTVMTrdejdzN1nTlxxRj2oT3aTjrQraqb9_vLtUF-FaMVGuQmgRPN8JYvgx25TV5FJvx1F7G-akgIuaV4RxXtBn_6BXYY6-xCsUl6JqgMIddaFHq5wfwnK6RarWDZAKOKkX18st1ceQUrTDfmUgailYLQWrXcEFf_pnzD38u9ECvNgCpT-jb9x_6mxh7KDvaCCspqz6BU8Yt40</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wang, Yuanjun</creator><creator>Fu, Chongluo</creator><creator>Li, Aifeng</creator><creator>Wang, Yuehua</creator><creator>Xuan, Hongzhuan</creator><creator>Peng, Wenjun</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control</title><author>Wang, Yuanjun ; Fu, Chongluo ; Li, Aifeng ; Wang, Yuehua ; Xuan, Hongzhuan ; Peng, Wenjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-171d78674da8b5dde924000c5d9be5d4eb826d414089b0deac8a4d9282b3a1fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino acids</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Cell growth</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>High performance liquid chromatography</topic><topic>Laboratories</topic><topic>Polyphenols</topic><topic>Quality control</topic><topic>Quality management</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yuanjun</creatorcontrib><creatorcontrib>Fu, Chongluo</creatorcontrib><creatorcontrib>Li, Aifeng</creatorcontrib><creatorcontrib>Wang, Yuehua</creatorcontrib><creatorcontrib>Xuan, Hongzhuan</creatorcontrib><creatorcontrib>Peng, Wenjun</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yuanjun</au><au>Fu, Chongluo</au><au>Li, Aifeng</au><au>Wang, Yuehua</au><au>Xuan, Hongzhuan</au><au>Peng, Wenjun</au><au>Savka, Michael A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>2016</volume><issue>2016</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist “bioactive components,” which also provides a new idea for Chinese propolis quality control.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>27123037</pmid><doi>10.1155/2016/9641965</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley-Blackwell Open Access Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Amino acids Antioxidants Apoptosis Breast cancer Cell growth Ethanol Flavonoids High performance liquid chromatography Laboratories Polyphenols Quality control Quality management Studies |
| title | Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control |
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