Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo
Recent findings have revealed that the female gonad may have regenerative activity with having germ line stem cells in juveniles and adults. Application of these germ line stem cells could be an alternative therapy for reproductive disorders in regenerative medicine. To enhance the potency of differ...
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| Veröffentlicht in: | Journal of ovarian research 2016-04, Vol.9 (24), p.24-24, Article 24 |
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| creator | Lee, Yeon-Mi Kim, Tae-Ho Lee, Jeong-Hyeon Lee, Won-Jae Jeon, Ryoung-Hoon Jang, Si-Jung Ock, Sun-A Lee, Sung-Lim Park, Bong-Wook Rho, Gyu-Jin |
| description | Recent findings have revealed that the female gonad may have regenerative activity with having germ line stem cells in juveniles and adults. Application of these germ line stem cells could be an alternative therapy for reproductive disorders in regenerative medicine.
To enhance the potency of differentiation into oocyte-like cells (OLCs) and folliculogenesis, we overexpressed Oct4 in ovarian stem/stromal cell (OvSCs) and examined the cellular properties related to stemness and self-renewal ability and finally demonstrated the ability of in vitro differentiation and folliculogenesis.
Ovarian cortex included putative stem cells in terms of AP activity, cell cycle status, cell proliferation, expression of mesenchymal lineage surface markers and pluripotent transcriptional markers. Further, Oct4 transfected OvSCs (Oct4-OvSCs) were enhanced their AP activity and cell proliferation compared to OvSCs. The potential on in vitro differentiation into OLCs and in vivo folliculogenesis was also evaluated in OvSCs and Oct4-OvSCs, respectively. Oct4-OvSCs possessed higher oogenesis potential in vitro than OvSCs, in terms of expression of germ cell markers by RT-PCR and the number of OLCs. When OvSCs and Oct4-OvSCs were xeno-transplanted into infertile mice ovaries, the OvSCs transplantation induced new primary follicle formation and hormonal levels of estradiol and FSH remained similar to that of normal mice. However, Oct4-OvSCs possessed higher ability for folliculogenesis based on inducing developing follicles with thecal layer and granulosa cells and more similar estradiol level to normal mice.
These findings demonstrated that putative stem cells were present in ovarian cortex and exhibited differentiation ability into OLCs and folliculogenesis in vivo, and Oct4-overexpression enhanced these ability, suggesting their cellular models based on gene therapy in understanding the mechanisms of oogenesis and folliculogenesis, and finally in view of reproductive cell therapy. |
| doi_str_mv | 10.1186/s13048-016-0233-z |
| format | Article |
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To enhance the potency of differentiation into oocyte-like cells (OLCs) and folliculogenesis, we overexpressed Oct4 in ovarian stem/stromal cell (OvSCs) and examined the cellular properties related to stemness and self-renewal ability and finally demonstrated the ability of in vitro differentiation and folliculogenesis.
Ovarian cortex included putative stem cells in terms of AP activity, cell cycle status, cell proliferation, expression of mesenchymal lineage surface markers and pluripotent transcriptional markers. Further, Oct4 transfected OvSCs (Oct4-OvSCs) were enhanced their AP activity and cell proliferation compared to OvSCs. The potential on in vitro differentiation into OLCs and in vivo folliculogenesis was also evaluated in OvSCs and Oct4-OvSCs, respectively. Oct4-OvSCs possessed higher oogenesis potential in vitro than OvSCs, in terms of expression of germ cell markers by RT-PCR and the number of OLCs. When OvSCs and Oct4-OvSCs were xeno-transplanted into infertile mice ovaries, the OvSCs transplantation induced new primary follicle formation and hormonal levels of estradiol and FSH remained similar to that of normal mice. However, Oct4-OvSCs possessed higher ability for folliculogenesis based on inducing developing follicles with thecal layer and granulosa cells and more similar estradiol level to normal mice.
These findings demonstrated that putative stem cells were present in ovarian cortex and exhibited differentiation ability into OLCs and folliculogenesis in vivo, and Oct4-overexpression enhanced these ability, suggesting their cellular models based on gene therapy in understanding the mechanisms of oogenesis and folliculogenesis, and finally in view of reproductive cell therapy.</description><identifier>ISSN: 1757-2215</identifier><identifier>EISSN: 1757-2215</identifier><identifier>DOI: 10.1186/s13048-016-0233-z</identifier><identifier>PMID: 27067537</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Animals ; Biomarkers - metabolism ; Cell Differentiation ; Cell Proliferation ; Cell Shape ; Cells, Cultured ; Estradiol ; Estradiol - blood ; Female ; Follicle Stimulating Hormone - blood ; Gene Expression ; Infertility, Female - blood ; Infertility, Female - therapy ; Mice, Inbred BALB C ; Mice, Nude ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Oocytes - physiology ; Ovarian Follicle - pathology ; Physiological aspects ; Stem Cell Transplantation ; Stem cells ; Stem Cells - physiology ; Sus scrofa ; Transplantation</subject><ispartof>Journal of ovarian research, 2016-04, Vol.9 (24), p.24-24, Article 24</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>Lee et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-adbc81b5c0c80683d260c8926c9bc93bbf9749378f0eb5305d120fcd558a38773</citedby><cites>FETCH-LOGICAL-c591t-adbc81b5c0c80683d260c8926c9bc93bbf9749378f0eb5305d120fcd558a38773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828771/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828771/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27067537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Yeon-Mi</creatorcontrib><creatorcontrib>Kim, Tae-Ho</creatorcontrib><creatorcontrib>Lee, Jeong-Hyeon</creatorcontrib><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Jeon, Ryoung-Hoon</creatorcontrib><creatorcontrib>Jang, Si-Jung</creatorcontrib><creatorcontrib>Ock, Sun-A</creatorcontrib><creatorcontrib>Lee, Sung-Lim</creatorcontrib><creatorcontrib>Park, Bong-Wook</creatorcontrib><creatorcontrib>Rho, Gyu-Jin</creatorcontrib><title>Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo</title><title>Journal of ovarian research</title><addtitle>J Ovarian Res</addtitle><description>Recent findings have revealed that the female gonad may have regenerative activity with having germ line stem cells in juveniles and adults. Application of these germ line stem cells could be an alternative therapy for reproductive disorders in regenerative medicine.
To enhance the potency of differentiation into oocyte-like cells (OLCs) and folliculogenesis, we overexpressed Oct4 in ovarian stem/stromal cell (OvSCs) and examined the cellular properties related to stemness and self-renewal ability and finally demonstrated the ability of in vitro differentiation and folliculogenesis.
Ovarian cortex included putative stem cells in terms of AP activity, cell cycle status, cell proliferation, expression of mesenchymal lineage surface markers and pluripotent transcriptional markers. Further, Oct4 transfected OvSCs (Oct4-OvSCs) were enhanced their AP activity and cell proliferation compared to OvSCs. The potential on in vitro differentiation into OLCs and in vivo folliculogenesis was also evaluated in OvSCs and Oct4-OvSCs, respectively. Oct4-OvSCs possessed higher oogenesis potential in vitro than OvSCs, in terms of expression of germ cell markers by RT-PCR and the number of OLCs. When OvSCs and Oct4-OvSCs were xeno-transplanted into infertile mice ovaries, the OvSCs transplantation induced new primary follicle formation and hormonal levels of estradiol and FSH remained similar to that of normal mice. However, Oct4-OvSCs possessed higher ability for folliculogenesis based on inducing developing follicles with thecal layer and granulosa cells and more similar estradiol level to normal mice.
These findings demonstrated that putative stem cells were present in ovarian cortex and exhibited differentiation ability into OLCs and folliculogenesis in vivo, and Oct4-overexpression enhanced these ability, suggesting their cellular models based on gene therapy in understanding the mechanisms of oogenesis and folliculogenesis, and finally in view of reproductive cell therapy.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Estradiol</subject><subject>Estradiol - blood</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Gene Expression</subject><subject>Infertility, Female - blood</subject><subject>Infertility, Female - therapy</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Oocytes - physiology</subject><subject>Ovarian Follicle - pathology</subject><subject>Physiological aspects</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - physiology</subject><subject>Sus scrofa</subject><subject>Transplantation</subject><issn>1757-2215</issn><issn>1757-2215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptks1u1DAUhSMEoqXwAGxQJCTEJq1_4tjZIFUVf1Kl2cDacpzrjotjD3YStX0WHhanMwwzCHnhK_s7x75XpyheY3SOsWguEqaoFhXCTYUIpdXDk-IUc8YrQjB7elCfFC9SukWoIaKmz4sTwlHDGeWnxa_VDBHuNhFSssGXwZQrPdal9eUmRG09lGFW0SpfphGGizTGMChXanAuleDXymtIZW-NyT5-tGrc2YSg70eonP0BOzp7zjbrS-X7vasJzlk9ORVzGYet_JGcw8vimVEuwavdflZ8__Tx29WX6nr1-evV5XWlWYvHSvWdFrhjGmmBGkF70uSqJY1uO93SrjMtr1vKhUHQMYpYjwkyumdMKCo4p2fFh63vZuoG6HXuIyonN9EOKt7LoKw8vvF2LW_CLGtBsh5ng_c7gxh-TpBGOdi0NK08hClJzAUSGHHcZvTtP-htmKLP7WWq5Q3DVOC_1I1yIK03Ib-rF1N5WdctYaR-_Pf5f6i8ehisDh6MzedHgncHgjUoN65TcNMy9HQM4i2oY0gpgtkPAyO5ZE9usydz9uSSPfmQNW8Op7hX_Akb_Q1YQNb7</recordid><startdate>20160412</startdate><enddate>20160412</enddate><creator>Lee, Yeon-Mi</creator><creator>Kim, Tae-Ho</creator><creator>Lee, Jeong-Hyeon</creator><creator>Lee, Won-Jae</creator><creator>Jeon, Ryoung-Hoon</creator><creator>Jang, Si-Jung</creator><creator>Ock, Sun-A</creator><creator>Lee, Sung-Lim</creator><creator>Park, Bong-Wook</creator><creator>Rho, Gyu-Jin</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160412</creationdate><title>Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo</title><author>Lee, Yeon-Mi ; Kim, Tae-Ho ; Lee, Jeong-Hyeon ; Lee, Won-Jae ; Jeon, Ryoung-Hoon ; Jang, Si-Jung ; Ock, Sun-A ; Lee, Sung-Lim ; Park, Bong-Wook ; Rho, Gyu-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c591t-adbc81b5c0c80683d260c8926c9bc93bbf9749378f0eb5305d120fcd558a38773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell Shape</topic><topic>Cells, Cultured</topic><topic>Estradiol</topic><topic>Estradiol - blood</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Gene Expression</topic><topic>Infertility, Female - blood</topic><topic>Infertility, Female - therapy</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Oocytes - physiology</topic><topic>Ovarian Follicle - pathology</topic><topic>Physiological aspects</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - physiology</topic><topic>Sus scrofa</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Yeon-Mi</creatorcontrib><creatorcontrib>Kim, Tae-Ho</creatorcontrib><creatorcontrib>Lee, Jeong-Hyeon</creatorcontrib><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Jeon, Ryoung-Hoon</creatorcontrib><creatorcontrib>Jang, Si-Jung</creatorcontrib><creatorcontrib>Ock, Sun-A</creatorcontrib><creatorcontrib>Lee, Sung-Lim</creatorcontrib><creatorcontrib>Park, Bong-Wook</creatorcontrib><creatorcontrib>Rho, Gyu-Jin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of ovarian research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Yeon-Mi</au><au>Kim, Tae-Ho</au><au>Lee, Jeong-Hyeon</au><au>Lee, Won-Jae</au><au>Jeon, Ryoung-Hoon</au><au>Jang, Si-Jung</au><au>Ock, Sun-A</au><au>Lee, Sung-Lim</au><au>Park, Bong-Wook</au><au>Rho, Gyu-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo</atitle><jtitle>Journal of ovarian research</jtitle><addtitle>J Ovarian Res</addtitle><date>2016-04-12</date><risdate>2016</risdate><volume>9</volume><issue>24</issue><spage>24</spage><epage>24</epage><pages>24-24</pages><artnum>24</artnum><issn>1757-2215</issn><eissn>1757-2215</eissn><abstract>Recent findings have revealed that the female gonad may have regenerative activity with having germ line stem cells in juveniles and adults. Application of these germ line stem cells could be an alternative therapy for reproductive disorders in regenerative medicine.
To enhance the potency of differentiation into oocyte-like cells (OLCs) and folliculogenesis, we overexpressed Oct4 in ovarian stem/stromal cell (OvSCs) and examined the cellular properties related to stemness and self-renewal ability and finally demonstrated the ability of in vitro differentiation and folliculogenesis.
Ovarian cortex included putative stem cells in terms of AP activity, cell cycle status, cell proliferation, expression of mesenchymal lineage surface markers and pluripotent transcriptional markers. Further, Oct4 transfected OvSCs (Oct4-OvSCs) were enhanced their AP activity and cell proliferation compared to OvSCs. The potential on in vitro differentiation into OLCs and in vivo folliculogenesis was also evaluated in OvSCs and Oct4-OvSCs, respectively. Oct4-OvSCs possessed higher oogenesis potential in vitro than OvSCs, in terms of expression of germ cell markers by RT-PCR and the number of OLCs. When OvSCs and Oct4-OvSCs were xeno-transplanted into infertile mice ovaries, the OvSCs transplantation induced new primary follicle formation and hormonal levels of estradiol and FSH remained similar to that of normal mice. However, Oct4-OvSCs possessed higher ability for folliculogenesis based on inducing developing follicles with thecal layer and granulosa cells and more similar estradiol level to normal mice.
These findings demonstrated that putative stem cells were present in ovarian cortex and exhibited differentiation ability into OLCs and folliculogenesis in vivo, and Oct4-overexpression enhanced these ability, suggesting their cellular models based on gene therapy in understanding the mechanisms of oogenesis and folliculogenesis, and finally in view of reproductive cell therapy.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27067537</pmid><doi>10.1186/s13048-016-0233-z</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of ovarian research, 2016-04, Vol.9 (24), p.24-24, Article 24 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Analysis Animals Biomarkers - metabolism Cell Differentiation Cell Proliferation Cell Shape Cells, Cultured Estradiol Estradiol - blood Female Follicle Stimulating Hormone - blood Gene Expression Infertility, Female - blood Infertility, Female - therapy Mice, Inbred BALB C Mice, Nude Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Oocytes - physiology Ovarian Follicle - pathology Physiological aspects Stem Cell Transplantation Stem cells Stem Cells - physiology Sus scrofa Transplantation |
| title | Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo |
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