Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

This protocol presents a method to perform quantitative, single-cell in situ analyses of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for embryo collection, immunofluorescence, imaging on a confocal microscope, and image segmentation an...

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Veröffentlicht in:	Journal of Visualized Experiments 2016-02 (108), p.53654-53654
Hauptverfasser:	Saiz, Nestor, Kang, Minjung, Schrode, Nadine, Lou, Xinghua, Hadjantonakis, Anna-Katerina
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst - physiology Developmental Biology Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Fluorescent Antibody Technique Mice Microscopy - methods Protein Processing, Post-Translational - physiology Proteins - metabolism Proteomics - methods Software
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