The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands
This study developed an animal model of surgically wounded submandibular glands （SMGs） and investigated the effects of collagen gel with basic fibroblast growth factor （bFGF） on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a...
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| Veröffentlicht in: | International journal of oral science 2016-03, Vol.8 (1), p.16-23 |
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| creator | Kobayashi, Fumitaka Matsuzaka, Kenichi Inoue, Takashi |
| description | This study developed an animal model of surgically wounded submandibular glands （SMGs） and investigated the effects of collagen gel with basic fibroblast growth factor （bFGF） on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF （bFGF group） or without bFGF （control group）. In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day （AOD） 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, g-smooth muscle actin （αSMA）, CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin 19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration. |
| doi_str_mv | 10.1038/ijos.2015.36 |
| format | Article |
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The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF （bFGF group） or without bFGF （control group）. In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day （AOD） 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, g-smooth muscle actin （αSMA）, CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin 19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.</description><identifier>ISSN: 1674-2818</identifier><identifier>EISSN: 2049-3169</identifier><identifier>DOI: 10.1038/ijos.2015.36</identifier><identifier>PMID: 27025261</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Collagen - pharmacology ; Dentistry ; Disease Models, Animal ; Fibroblast Growth Factor 2 - pharmacology ; Gels ; Immunohistochemistry ; Male ; Medicine ; Oral and Maxillofacial Surgery ; Original ; original-article ; Orthopedics ; Rats ; Rats, Sprague-Dawley ; Regeneration - drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; Submandibular Gland - surgery ; Surgical Orthopedics ; Wound Healing - drug effects ; 伤口 ; 动物模型 ; 大鼠 ; 手术 ; 碱性成纤维细胞生长因子 ; 组织再生 ; 胶原凝胶 ; 颌下腺</subject><ispartof>International journal of oral science, 2016-03, Vol.8 (1), p.16-23</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Mar 2016</rights><rights>Copyright © Wanfang Data Co. 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All Rights Reserved.</rights><rights>Copyright © 2015 West China School of Stomatology 2015 West China School of Stomatology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c623t-4d0e51200c1b55e11779d1822b5fd07cf5837ed92ecf2239c14a77586021c9bd3</citedby><cites>FETCH-LOGICAL-c623t-4d0e51200c1b55e11779d1822b5fd07cf5837ed92ecf2239c14a77586021c9bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/89520X/89520X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822181/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822181/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27025261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Fumitaka</creatorcontrib><creatorcontrib>Matsuzaka, Kenichi</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><title>The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands</title><title>International journal of oral science</title><addtitle>Int J Oral Sci</addtitle><addtitle>International Journal of Oral Science</addtitle><description>This study developed an animal model of surgically wounded submandibular glands （SMGs） and investigated the effects of collagen gel with basic fibroblast growth factor （bFGF） on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF （bFGF group） or without bFGF （control group）. In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day （AOD） 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, g-smooth muscle actin （αSMA）, CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin 19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.</description><subject>Animals</subject><subject>Collagen - pharmacology</subject><subject>Dentistry</subject><subject>Disease Models, Animal</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Gels</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medicine</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original</subject><subject>original-article</subject><subject>Orthopedics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration - drug effects</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Submandibular Gland - surgery</subject><subject>Surgical Orthopedics</subject><subject>Wound Healing - drug effects</subject><subject>伤口</subject><subject>动物模型</subject><subject>大鼠</subject><subject>手术</subject><subject>碱性成纤维细胞生长因子</subject><subject>组织再生</subject><subject>胶原凝胶</subject><subject>颌下腺</subject><issn>1674-2818</issn><issn>2049-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkc9vFCEcxYnR2LV682yIXkzqrPwYfl1MTGPVpImXeiYMA7NsZ6ELM672r5fNrptqPAH5fnjvwQPgJUZLjKh8H9apLAnCbEn5I7AgqFUNxVw9BgvMRdsQieUZeFbKGiEuGcFPwRkRiDDC8QLkm5WDzntnJ5g87EwJFvrQ5dSNpkxwyGk3raA3dkoZpgizG1x02UyhHkKEBpY5D8GaEe7SHHu4Sb0b91qVqbNuY2Ifunk0GQ5j3Zfn4Ik3Y3Evjus5-H716ebyS3P97fPXy4_XjeWETk3bI8cwQcjijjGHsRCqx5KQjvkeCeuZpML1ijjrCaHK4tYIwSRHBFvV9fQcfDjo3tUQrrcuTtmM-i6Hjcm_dDJB_z2JYaWH9EO31QRLXAUuDgI7E72Jg16nOccaWQ_r2-3tz_t77erHc4QRopV-e7TLaTu7MulNKNaN9c0uzUXX_KKVQrW8om_-QU_KWCglGVNYVurdgbI5lZKdP0XHSO-r1_vq9b56Tfeirx4-9wT_6boCzQEodRQHlx-4_l_w9dF_leKwrVdOmpxLzjiniv4GMFLGCw</recordid><startdate>20160330</startdate><enddate>20160330</enddate><creator>Kobayashi, Fumitaka</creator><creator>Matsuzaka, Kenichi</creator><creator>Inoue, Takashi</creator><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><general>Department of Clinical Pathophysiology, Tokyo Dental Col ege, Tokyo, Japan</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20160330</creationdate><title>The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands</title><author>Kobayashi, Fumitaka ; Matsuzaka, Kenichi ; Inoue, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c623t-4d0e51200c1b55e11779d1822b5fd07cf5837ed92ecf2239c14a77586021c9bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Collagen - pharmacology</topic><topic>Dentistry</topic><topic>Disease Models, Animal</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Gels</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medicine</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original</topic><topic>original-article</topic><topic>Orthopedics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration - drug effects</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Submandibular Gland - surgery</topic><topic>Surgical Orthopedics</topic><topic>Wound Healing - drug effects</topic><topic>伤口</topic><topic>动物模型</topic><topic>大鼠</topic><topic>手术</topic><topic>碱性成纤维细胞生长因子</topic><topic>组织再生</topic><topic>胶原凝胶</topic><topic>颌下腺</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Fumitaka</creatorcontrib><creatorcontrib>Matsuzaka, Kenichi</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of oral science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Fumitaka</au><au>Matsuzaka, Kenichi</au><au>Inoue, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands</atitle><jtitle>International journal of oral science</jtitle><stitle>Int J Oral Sci</stitle><addtitle>International Journal of Oral Science</addtitle><date>2016-03-30</date><risdate>2016</risdate><volume>8</volume><issue>1</issue><spage>16</spage><epage>23</epage><pages>16-23</pages><issn>1674-2818</issn><eissn>2049-3169</eissn><abstract>This study developed an animal model of surgically wounded submandibular glands （SMGs） and investigated the effects of collagen gel with basic fibroblast growth factor （bFGF） on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF （bFGF group） or without bFGF （control group）. In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day （AOD） 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, g-smooth muscle actin （αSMA）, CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin 19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27025261</pmid><doi>10.1038/ijos.2015.36</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Collagen - pharmacology Dentistry Disease Models, Animal Fibroblast Growth Factor 2 - pharmacology Gels Immunohistochemistry Male Medicine Oral and Maxillofacial Surgery Original original-article Orthopedics Rats Rats, Sprague-Dawley Regeneration - drug effects Reverse Transcriptase Polymerase Chain Reaction Submandibular Gland - surgery Surgical Orthopedics Wound Healing - drug effects 伤口 动物模型 大鼠 手术 碱性成纤维细胞生长因子 组织再生 胶原凝胶 颌下腺 |
| title | The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands |
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