3β-hydroxysteroid dehydrogenase isoforms in human aldosterone-producing adenoma

•The level of HSD3B2 expression was significantly higher than that of HSD3B1 in APA.•The level of HSD3Β1 mRNA was positively correlated with that of CYP11B2 in APA.•The level of HSD3B1 mRNA was significantly correlated with PAC in APA.•H-score of HSD3Β1 was significantly correlated with that of CYP1...

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Veröffentlicht in:	Molecular and cellular endocrinology 2015-06, Vol.408, p.205-212
Hauptverfasser:	Konosu-Fukaya, Sachiko, Nakamura, Yasuhiro, Satoh, Fumitoshi, Felizola, Saulo J.A., Maekawa, Takashi, Ono, Yoshikiyo, Morimoto, Ryo, Ise, Kazue, Takeda, Ken-ichiro, Katsu, Koshin, Fujishima, Fumiyoshi, Kasajima, Atsuko, Watanabe, Mika, Arai, Yoichi, Gomez-Sanchez, Elise P., Gomez-Sanchez, Celso E., Doi, Masao, Okamura, Hitoshi, Sasano, Hironobu
Format:	Artikel
Sprache:	eng
Schlagworte:	3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) 3β-hydroxysteroid dehydrogenase type 2 (HSD3B2) adenoma Adenoma - enzymology Adenoma - genetics Adenoma - pathology aldosterone Aldosterone - biosynthesis Aldosterone-producing adenoma (APA) CYP11B1 (11β-hydroxylase) and KCNJ5 CYP11B2 (aldosterone synthase) Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Isoenzymes - genetics Isoenzymes - metabolism messenger RNA Multienzyme Complexes - genetics Multienzyme Complexes - metabolism mutation patients Progesterone Reductase - genetics Progesterone Reductase - metabolism quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction Steroid Isomerases - genetics Steroid Isomerases - metabolism
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Zusammenfassung:	•The level of HSD3B2 expression was significantly higher than that of HSD3B1 in APA.•The level of HSD3Β1 mRNA was positively correlated with that of CYP11B2 in APA.•The level of HSD3B1 mRNA was significantly correlated with PAC in APA.•H-score of HSD3Β1 was significantly correlated with that of CYP11B2 in APA.•The level of HSD3B1 mRNA was higher in KCNJ5 mutated APA than in wild type cases. It has become important to evaluate the possible involvement of 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) and 2 (HSD3B2) isoforms in aldosterone-producing adenoma (APA). In this study, we studied 67 and 100 APA cases using real-time quantitative PCR (qPCR) and immunohistochemistry, respectively. Results of qPCR analysis demonstrated that HSD3B2 mRNA was significantly more abundant than HSD3B1 mRNA (P
ISSN:	0303-7207 1872-8057
DOI:	10.1016/j.mce.2014.10.008