Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection

Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired mutation, several experimental options exist in the isolation of clonal lines, such as selec...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	BMC molecular biology 2016-04, Vol.17 (9), p.9-9, Article 9
Hauptverfasser:	Estep, Jason A, Sternburg, Erin L, Sanchez, Gissell A, Karginov, Fedor V
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Base Sequence Cell Line Cells CRISPR-Cas Systems ELAV-Like Protein 1 - chemistry ELAV-Like Protein 1 - genetics Gene expression Gene Knockout Techniques Gene Targeting - methods Genotype Humans Immunoblotting Methodology Molecular Sequence Data Mutation RNA, Guide, CRISPR-Cas Systems - chemistry RNA, Guide, CRISPR-Cas Systems - genetics Sequence Alignment
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!