Deglycosylation of Tropheryma whipplei biofilm and discrepancies between diagnostic results during Whipple’s disease progression

Whipple’s disease is a systemic infectious disease associated with the bacterium Tropheryma whipplei . Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 du...

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Veröffentlicht in:	Scientific reports 2016-03, Vol.6 (1), p.23883-23883, Article 23883
Hauptverfasser:	Audoly, Gilles, Fenollar, Florence, Lagier, Jean-Christophe, Lepidi, Hubert, Raoult, Didier
Format:	Artikel
Sprache:	eng
Schlagworte:	13/51 14 14/32 14/63 38 631/326/107 692/4020/1503/1581/257 692/420/254 Alveoli Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Bacteria Bacteriolysis Biofilms Biopsy Bronchus Deglycosylation Deoxyribonucleic acid Disease Progression DNA Duodenum - microbiology Duodenum - pathology Epithelial cells Fluorescence in situ hybridization Glycoside Hydrolases - chemistry Glycoside Hydrolases - pharmacology Glycosylation Human health and pathology Humanities and Social Sciences Humans Hydrolysis Immunofluorescence Infectious diseases Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Life Sciences Localization Lymph Lymph nodes Lymph Nodes - microbiology Lymph Nodes - pathology Macrophages Microscopy, Confocal multidisciplinary Ribonucleic acid RNA Science Tropheryma - physiology Tropheryma whipplei Whipple Disease - diagnosis Whipple Disease - drug therapy Whipple Disease - microbiology Whipple's disease
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creator	Audoly, Gilles Fenollar, Florence Lagier, Jean-Christophe Lepidi, Hubert Raoult, Didier
description	Whipple’s disease is a systemic infectious disease associated with the bacterium Tropheryma whipplei . Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and 1 lymph node biopsy from Whipple’s patients. We showed the presence of bacteria in both CK20 + epithelial cells and CD68 + macrophages. Bacteria are found embedded in a biofilm hindering the detection of T. whipplei . Only after treatment of biopsies by glycosidases, co-localization of T. whipplei RNA/DNA with bacterial proteins was restored. Moreover, using 13 bronchoalveolar lavages and 7 duodenal biopsies, we found that hydrolysis of the biofilm weakened the bacteria, facilitated bacterial DNA extraction and improved the sensitivity of qPCR detection by up to 1000x opening new perspectives for diagnostic and scientific approaches.
doi_str_mv	10.1038/srep23883
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Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and 1 lymph node biopsy from Whipple’s patients. We showed the presence of bacteria in both CK20 + epithelial cells and CD68 + macrophages. Bacteria are found embedded in a biofilm hindering the detection of T. whipplei . Only after treatment of biopsies by glycosidases, co-localization of T. whipplei RNA/DNA with bacterial proteins was restored. 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Audoly, Gilles</au><au>Fenollar, Florence</au><au>Lagier, Jean-Christophe</au><au>Lepidi, Hubert</au><au>Raoult, Didier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deglycosylation of Tropheryma whipplei biofilm and discrepancies between diagnostic results during Whipple’s disease progression</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-03-30</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>23883</spage><epage>23883</epage><pages>23883-23883</pages><artnum>23883</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Whipple’s disease is a systemic infectious disease associated with the bacterium Tropheryma whipplei . Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and 1 lymph node biopsy from Whipple’s patients. We showed the presence of bacteria in both CK20 + epithelial cells and CD68 + macrophages. Bacteria are found embedded in a biofilm hindering the detection of T. whipplei . Only after treatment of biopsies by glycosidases, co-localization of T. whipplei RNA/DNA with bacterial proteins was restored. 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subjects	13/51 14 14/32 14/63 38 631/326/107 692/4020/1503/1581/257 692/420/254 Alveoli Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Bacteria Bacteriolysis Biofilms Biopsy Bronchus Deglycosylation Deoxyribonucleic acid Disease Progression DNA Duodenum - microbiology Duodenum - pathology Epithelial cells Fluorescence in situ hybridization Glycoside Hydrolases - chemistry Glycoside Hydrolases - pharmacology Glycosylation Human health and pathology Humanities and Social Sciences Humans Hydrolysis Immunofluorescence Infectious diseases Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Life Sciences Localization Lymph Lymph nodes Lymph Nodes - microbiology Lymph Nodes - pathology Macrophages Microscopy, Confocal multidisciplinary Ribonucleic acid RNA Science Tropheryma - physiology Tropheryma whipplei Whipple Disease - diagnosis Whipple Disease - drug therapy Whipple Disease - microbiology Whipple's disease
title	Deglycosylation of Tropheryma whipplei biofilm and discrepancies between diagnostic results during Whipple’s disease progression
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