Random transposon mutagenesis of the Saccharopolyspora erythraea genome reveals additional genes influencing erythromycin biosynthesis

A single cycle of strain improvement was performed in Saccharopolyspora erythraea mutB and 15 genotypes influencing erythromycin production were found. Genotypes generated by transposon mutagenesis appeared in the screen at a frequency of ∼3%. Mutations affecting central metabolism and regulatory ge...

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Veröffentlicht in:	FEMS microbiology letters 2015-11, Vol.362 (22), p.fnv180
Hauptverfasser:	Fedashchin, Andrij, Cernota, William H., Gonzalez, Melissa C., Leach, Benjamin I., Kwan, Noelle, Wesley, Roy K., Weber, J. Mark
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - biosynthesis Antibiotics Biosynthesis Biosynthetic Pathways - genetics Biotechnology & Synthetic Biology Cell culture DNA Transposable Elements Erythromycin Erythromycin - biosynthesis Flasks Genes Genes, Bacterial Genome, Bacterial Genomes Genotypes Hydrolase Metabolic Engineering - methods Microbiology Mutagenesis Mutagenesis, Insertional Mutation Peptidases Research Letter Saccharopolyspora - genetics Saccharopolyspora - metabolism Saccharopolyspora erythraea Transposon mutagenesis Transposons
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