Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer
Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis is the key event in SCLC progression, but its mechanism has not been fully elucidated. MicroRNAs (miRNAs) have been proven to participate in cancer processes, but their...
Gespeichert in:
| Veröffentlicht in: | Oncotarget 2015-12, Vol.6 (42), p.44609-44622 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 44622 |
|---|---|
| container_issue | 42 |
| container_start_page | 44609 |
| container_title | Oncotarget |
| container_volume | 6 |
| creator | Zhou, Rui Zhou, Xiaoshu Yin, Zhongyuan Guo, Jing Hu, Ting Jiang, Shun Liu, Li Dong, Xiaorong Zhang, Sheng Wu, Gang |
| description | Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis is the key event in SCLC progression, but its mechanism has not been fully elucidated. MicroRNAs (miRNAs) have been proven to participate in cancer processes, but their function in SCLC has not been thoroughly studied either. Here, we performed microarray and quantitative real-time PCR (qRT-PCR) analyses to identify the miRNAs associated with metastasis and prognosis in SCLC as well as the correlation between serum and tissue. We also explored these miRNAs' promising molecular mechanisms by 3'UTR reporter assay and immunoblotting. We showed that miR-184 significantly attenuated the metastasis of SCLC, whereas miR-574-5p enhanced it. Both miRNAs were found to participate in β-catenin signaling by suppressing protein tyrosine phosphatase receptor type U (PTPRU) or endothelial PAS domain protein 1 (EPAS1). Furthermore, miR-574-5p was verified as an independent prognostic risk factor for SCLC. Taken together, our findings provide a comprehensive analysis of the miRNA expression pattern in SCLC and indicate that miRNAs may serve as potential therapeutic and prognostic predictors in SCLC. |
| doi_str_mv | 10.18632/oncotarget.6338 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4792579</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1770883873</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-bc1da6030c740d6cfc1597c2d738971195faa989078ad5e77189dc62426abdef3</originalsourceid><addsrcrecordid>eNpVkctLxDAQxoMorqh3T5Kjl2oeTZNchGXxBYuCrOcwm6RrpU3WpBX87-26PsNAwuSbLzP5IXRCyTlVFWcXMdjYQ1r5_rziXO2gA6pLXTAh-O6f8wQd5_xCxiVKqZjeRxNWCSUVJwfIL4YuJtyEN8hNDBiCw53vIY_RZJz8amih9w4v33HX2BQf76cFVeVW-J0QsizEenTBuYO2LaxvW9wOYYUtBOvTEdqroc3--Gs_RE_XV4vZbTF_uLmbTeeFLRnpi6WlDirCiZUlcZWtLRVaWuYkV1pSqkUNoJUmUoETXkqqtLMVK1kFS-drfogut77rYdl5Z33oE7RmnZoO0ruJ0Jj_N6F5Nqv4ZkqpmZB6NDj7MkjxdfC5N12TN9NA8HHIhkpJlOJK8lFKttLxD3JOvv55hhLzCcj8AjIbQGPJ6d_2fgq-cfAPjZ2PRQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770883873</pqid></control><display><type>article</type><title>Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer</title><source>Freely Accessible Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Zhou, Rui ; Zhou, Xiaoshu ; Yin, Zhongyuan ; Guo, Jing ; Hu, Ting ; Jiang, Shun ; Liu, Li ; Dong, Xiaorong ; Zhang, Sheng ; Wu, Gang</creator><creatorcontrib>Zhou, Rui ; Zhou, Xiaoshu ; Yin, Zhongyuan ; Guo, Jing ; Hu, Ting ; Jiang, Shun ; Liu, Li ; Dong, Xiaorong ; Zhang, Sheng ; Wu, Gang</creatorcontrib><description>Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis is the key event in SCLC progression, but its mechanism has not been fully elucidated. MicroRNAs (miRNAs) have been proven to participate in cancer processes, but their function in SCLC has not been thoroughly studied either. Here, we performed microarray and quantitative real-time PCR (qRT-PCR) analyses to identify the miRNAs associated with metastasis and prognosis in SCLC as well as the correlation between serum and tissue. We also explored these miRNAs' promising molecular mechanisms by 3'UTR reporter assay and immunoblotting. We showed that miR-184 significantly attenuated the metastasis of SCLC, whereas miR-574-5p enhanced it. Both miRNAs were found to participate in β-catenin signaling by suppressing protein tyrosine phosphatase receptor type U (PTPRU) or endothelial PAS domain protein 1 (EPAS1). Furthermore, miR-574-5p was verified as an independent prognostic risk factor for SCLC. Taken together, our findings provide a comprehensive analysis of the miRNA expression pattern in SCLC and indicate that miRNAs may serve as potential therapeutic and prognostic predictors in SCLC.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.6338</identifier><identifier>PMID: 26587830</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>3' Untranslated Regions ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; beta Catenin - genetics ; beta Catenin - metabolism ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Cell Line, Tumor ; Cell Movement ; Disease-Free Survival ; Female ; Gene Expression Regulation, Neoplastic ; HEK293 Cells ; Humans ; Kaplan-Meier Estimate ; Lung Neoplasms - blood ; Lung Neoplasms - genetics ; Lung Neoplasms - mortality ; Lung Neoplasms - pathology ; Lung Neoplasms - therapy ; Male ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; Neoplasm Invasiveness ; Proportional Hazards Models ; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics ; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism ; Research Paper ; Signal Transduction ; Small Cell Lung Carcinoma - blood ; Small Cell Lung Carcinoma - genetics ; Small Cell Lung Carcinoma - mortality ; Small Cell Lung Carcinoma - secondary ; Small Cell Lung Carcinoma - therapy ; Time Factors ; Transfection ; Treatment Outcome</subject><ispartof>Oncotarget, 2015-12, Vol.6 (42), p.44609-44622</ispartof><rights>Copyright: © 2015 Zhou et al. 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-bc1da6030c740d6cfc1597c2d738971195faa989078ad5e77189dc62426abdef3</citedby><cites>FETCH-LOGICAL-c420t-bc1da6030c740d6cfc1597c2d738971195faa989078ad5e77189dc62426abdef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792579/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792579/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26587830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Rui</creatorcontrib><creatorcontrib>Zhou, Xiaoshu</creatorcontrib><creatorcontrib>Yin, Zhongyuan</creatorcontrib><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Hu, Ting</creatorcontrib><creatorcontrib>Jiang, Shun</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Dong, Xiaorong</creatorcontrib><creatorcontrib>Zhang, Sheng</creatorcontrib><creatorcontrib>Wu, Gang</creatorcontrib><title>Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis is the key event in SCLC progression, but its mechanism has not been fully elucidated. MicroRNAs (miRNAs) have been proven to participate in cancer processes, but their function in SCLC has not been thoroughly studied either. Here, we performed microarray and quantitative real-time PCR (qRT-PCR) analyses to identify the miRNAs associated with metastasis and prognosis in SCLC as well as the correlation between serum and tissue. We also explored these miRNAs' promising molecular mechanisms by 3'UTR reporter assay and immunoblotting. We showed that miR-184 significantly attenuated the metastasis of SCLC, whereas miR-574-5p enhanced it. Both miRNAs were found to participate in β-catenin signaling by suppressing protein tyrosine phosphatase receptor type U (PTPRU) or endothelial PAS domain protein 1 (EPAS1). Furthermore, miR-574-5p was verified as an independent prognostic risk factor for SCLC. Taken together, our findings provide a comprehensive analysis of the miRNA expression pattern in SCLC and indicate that miRNAs may serve as potential therapeutic and prognostic predictors in SCLC.</description><subject>3' Untranslated Regions</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - metabolism</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Disease-Free Survival</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Lung Neoplasms - blood</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - mortality</subject><subject>Lung Neoplasms - pathology</subject><subject>Lung Neoplasms - therapy</subject><subject>Male</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Proportional Hazards Models</subject><subject>Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics</subject><subject>Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism</subject><subject>Research Paper</subject><subject>Signal Transduction</subject><subject>Small Cell Lung Carcinoma - blood</subject><subject>Small Cell Lung Carcinoma - genetics</subject><subject>Small Cell Lung Carcinoma - mortality</subject><subject>Small Cell Lung Carcinoma - secondary</subject><subject>Small Cell Lung Carcinoma - therapy</subject><subject>Time Factors</subject><subject>Transfection</subject><subject>Treatment Outcome</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctLxDAQxoMorqh3T5Kjl2oeTZNchGXxBYuCrOcwm6RrpU3WpBX87-26PsNAwuSbLzP5IXRCyTlVFWcXMdjYQ1r5_rziXO2gA6pLXTAh-O6f8wQd5_xCxiVKqZjeRxNWCSUVJwfIL4YuJtyEN8hNDBiCw53vIY_RZJz8amih9w4v33HX2BQf76cFVeVW-J0QsizEenTBuYO2LaxvW9wOYYUtBOvTEdqroc3--Gs_RE_XV4vZbTF_uLmbTeeFLRnpi6WlDirCiZUlcZWtLRVaWuYkV1pSqkUNoJUmUoETXkqqtLMVK1kFS-drfogut77rYdl5Z33oE7RmnZoO0ruJ0Jj_N6F5Nqv4ZkqpmZB6NDj7MkjxdfC5N12TN9NA8HHIhkpJlOJK8lFKttLxD3JOvv55hhLzCcj8AjIbQGPJ6d_2fgq-cfAPjZ2PRQ</recordid><startdate>20151229</startdate><enddate>20151229</enddate><creator>Zhou, Rui</creator><creator>Zhou, Xiaoshu</creator><creator>Yin, Zhongyuan</creator><creator>Guo, Jing</creator><creator>Hu, Ting</creator><creator>Jiang, Shun</creator><creator>Liu, Li</creator><creator>Dong, Xiaorong</creator><creator>Zhang, Sheng</creator><creator>Wu, Gang</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151229</creationdate><title>Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer</title><author>Zhou, Rui ; Zhou, Xiaoshu ; Yin, Zhongyuan ; Guo, Jing ; Hu, Ting ; Jiang, Shun ; Liu, Li ; Dong, Xiaorong ; Zhang, Sheng ; Wu, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-bc1da6030c740d6cfc1597c2d738971195faa989078ad5e77189dc62426abdef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3' Untranslated Regions</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - metabolism</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Disease-Free Survival</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Lung Neoplasms - blood</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - mortality</topic><topic>Lung Neoplasms - pathology</topic><topic>Lung Neoplasms - therapy</topic><topic>Male</topic><topic>MicroRNAs - blood</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Proportional Hazards Models</topic><topic>Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics</topic><topic>Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism</topic><topic>Research Paper</topic><topic>Signal Transduction</topic><topic>Small Cell Lung Carcinoma - blood</topic><topic>Small Cell Lung Carcinoma - genetics</topic><topic>Small Cell Lung Carcinoma - mortality</topic><topic>Small Cell Lung Carcinoma - secondary</topic><topic>Small Cell Lung Carcinoma - therapy</topic><topic>Time Factors</topic><topic>Transfection</topic><topic>Treatment Outcome</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Rui</creatorcontrib><creatorcontrib>Zhou, Xiaoshu</creatorcontrib><creatorcontrib>Yin, Zhongyuan</creatorcontrib><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Hu, Ting</creatorcontrib><creatorcontrib>Jiang, Shun</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Dong, Xiaorong</creatorcontrib><creatorcontrib>Zhang, Sheng</creatorcontrib><creatorcontrib>Wu, Gang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Rui</au><au>Zhou, Xiaoshu</au><au>Yin, Zhongyuan</au><au>Guo, Jing</au><au>Hu, Ting</au><au>Jiang, Shun</au><au>Liu, Li</au><au>Dong, Xiaorong</au><au>Zhang, Sheng</au><au>Wu, Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2015-12-29</date><risdate>2015</risdate><volume>6</volume><issue>42</issue><spage>44609</spage><epage>44622</epage><pages>44609-44622</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis is the key event in SCLC progression, but its mechanism has not been fully elucidated. MicroRNAs (miRNAs) have been proven to participate in cancer processes, but their function in SCLC has not been thoroughly studied either. Here, we performed microarray and quantitative real-time PCR (qRT-PCR) analyses to identify the miRNAs associated with metastasis and prognosis in SCLC as well as the correlation between serum and tissue. We also explored these miRNAs' promising molecular mechanisms by 3'UTR reporter assay and immunoblotting. We showed that miR-184 significantly attenuated the metastasis of SCLC, whereas miR-574-5p enhanced it. Both miRNAs were found to participate in β-catenin signaling by suppressing protein tyrosine phosphatase receptor type U (PTPRU) or endothelial PAS domain protein 1 (EPAS1). Furthermore, miR-574-5p was verified as an independent prognostic risk factor for SCLC. Taken together, our findings provide a comprehensive analysis of the miRNA expression pattern in SCLC and indicate that miRNAs may serve as potential therapeutic and prognostic predictors in SCLC.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>26587830</pmid><doi>10.18632/oncotarget.6338</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1949-2553 |
| ispartof | Oncotarget, 2015-12, Vol.6 (42), p.44609-44622 |
| issn | 1949-2553 1949-2553 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4792579 |
| source | Freely Accessible Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | 3' Untranslated Regions Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism beta Catenin - genetics beta Catenin - metabolism Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Cell Line, Tumor Cell Movement Disease-Free Survival Female Gene Expression Regulation, Neoplastic HEK293 Cells Humans Kaplan-Meier Estimate Lung Neoplasms - blood Lung Neoplasms - genetics Lung Neoplasms - mortality Lung Neoplasms - pathology Lung Neoplasms - therapy Male MicroRNAs - blood MicroRNAs - genetics Middle Aged Neoplasm Invasiveness Proportional Hazards Models Receptor-Like Protein Tyrosine Phosphatases, Class 2 - genetics Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism Research Paper Signal Transduction Small Cell Lung Carcinoma - blood Small Cell Lung Carcinoma - genetics Small Cell Lung Carcinoma - mortality Small Cell Lung Carcinoma - secondary Small Cell Lung Carcinoma - therapy Time Factors Transfection Treatment Outcome |
| title | Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T17%3A21%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tumor%20invasion%20and%20metastasis%20regulated%20by%20microRNA-184%20and%20microRNA-574-5p%20in%20small-cell%20lung%20cancer&rft.jtitle=Oncotarget&rft.au=Zhou,%20Rui&rft.date=2015-12-29&rft.volume=6&rft.issue=42&rft.spage=44609&rft.epage=44622&rft.pages=44609-44622&rft.issn=1949-2553&rft.eissn=1949-2553&rft_id=info:doi/10.18632/oncotarget.6338&rft_dat=%3Cproquest_pubme%3E1770883873%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770883873&rft_id=info:pmid/26587830&rfr_iscdi=true |