Notch Signaling in Meibomian Gland Epithelial Cell Differentiation
Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro. An immortalized human meibomian gland epithelial cell (HMGEC) lin...
Gespeichert in:
| Veröffentlicht in: | Investigative ophthalmology & visual science 2016-03, Vol.57 (3), p.859-865 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 865 |
|---|---|
| container_issue | 3 |
| container_start_page | 859 |
| container_title | Investigative ophthalmology & visual science |
| container_volume | 57 |
| creator | Gidfar, Sanaz Afsharkhamseh, Neda Sanjari, Sara Djalilian, Ali R |
| description | Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro.
An immortalized human meibomian gland epithelial cell (HMGEC) line was cultured under proliferative and differentiative conditions. Expression of Notch receptors and ligands were evaluated by quantitative PCR and Western blot. The effect of Notch inhibition and induction on oil production was also assessed.
Human meibomian gland epithelial cell expressed Notch1, Notch2, Notch3, Jagged1, Jagged2, Delta-like 1, and Delta-like 3. The level of cleaved (activated) Notch1 strongly increased with differentiation. The expression of Notch3 was inversely correlated with proliferation. Induction and inhibition of Notch1 led to an increase and decrease in the amount of oil production, respectively.
Notch signaling appears to play an important role in human meibomian gland epithelial differentiation and oil production. This may provide a potential therapeutic pathway for treating meibomian gland dysfunction. |
| doi_str_mv | 10.1167/iovs.15-18319 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4782822</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1771447188</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-564da7e59a68b47cb7a25779de8d03ea5c4876270d21297d985fbb023b2366033</originalsourceid><addsrcrecordid>eNpVkD1PwzAQhi0EoqUwsqKMLCk-f8TOggSlFKQCAzBbTuK0Rq5d4rQS_56UQlWmO-kevXf3IHQOeAiQiSsb1nEIPAVJIT9AfeCcpFxIerjX99BJjB8YEwCCj1GPZDmjwGQf3T6Htpwnr3bmtbN-llifPBlbhIXVPpk47atkvLTt3DirXTIyziV3tq5NY3xrdWuDP0VHtXbRnP3WAXq_H7-NHtLpy-RxdDNNS8Zpm_KMVVoYnutMFkyUhdCEC5FXRlaYGs1LJkVGBK4IkFxUueR1UWBCC0KzDFM6QNfb3OWqWJiq7A5otFPLxi5086WCtur_xNu5moW1YkISSUgXcPkb0ITPlYmtWthYdh9pb8IqKhACGBMgZYemW7RsQoyNqXdrAKuNd7XxroCrH-8df7F_247-E02_AZRufu0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1771447188</pqid></control><display><type>article</type><title>Notch Signaling in Meibomian Gland Epithelial Cell Differentiation</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Gidfar, Sanaz ; Afsharkhamseh, Neda ; Sanjari, Sara ; Djalilian, Ali R</creator><creatorcontrib>Gidfar, Sanaz ; Afsharkhamseh, Neda ; Sanjari, Sara ; Djalilian, Ali R</creatorcontrib><description>Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro.
An immortalized human meibomian gland epithelial cell (HMGEC) line was cultured under proliferative and differentiative conditions. Expression of Notch receptors and ligands were evaluated by quantitative PCR and Western blot. The effect of Notch inhibition and induction on oil production was also assessed.
Human meibomian gland epithelial cell expressed Notch1, Notch2, Notch3, Jagged1, Jagged2, Delta-like 1, and Delta-like 3. The level of cleaved (activated) Notch1 strongly increased with differentiation. The expression of Notch3 was inversely correlated with proliferation. Induction and inhibition of Notch1 led to an increase and decrease in the amount of oil production, respectively.
Notch signaling appears to play an important role in human meibomian gland epithelial differentiation and oil production. This may provide a potential therapeutic pathway for treating meibomian gland dysfunction.</description><identifier>ISSN: 1552-5783</identifier><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.15-18319</identifier><identifier>PMID: 26943148</identifier><language>eng</language><publisher>United States: The Association for Research in Vision and Ophthalmology</publisher><subject>Biochemistry and Molecular Biology ; Blotting, Western ; Cell Differentiation - genetics ; Cells, Cultured ; Epithelial Cells - pathology ; Eyelid Diseases - genetics ; Eyelid Diseases - metabolism ; Eyelid Diseases - pathology ; Gene Expression Regulation ; Humans ; Meibomian Glands - pathology ; Real-Time Polymerase Chain Reaction ; Receptor, Notch1 - biosynthesis ; Receptor, Notch1 - genetics ; RNA, Messenger - genetics ; Signal Transduction</subject><ispartof>Investigative ophthalmology & visual science, 2016-03, Vol.57 (3), p.859-865</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-564da7e59a68b47cb7a25779de8d03ea5c4876270d21297d985fbb023b2366033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782822/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782822/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26943148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gidfar, Sanaz</creatorcontrib><creatorcontrib>Afsharkhamseh, Neda</creatorcontrib><creatorcontrib>Sanjari, Sara</creatorcontrib><creatorcontrib>Djalilian, Ali R</creatorcontrib><title>Notch Signaling in Meibomian Gland Epithelial Cell Differentiation</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro.
An immortalized human meibomian gland epithelial cell (HMGEC) line was cultured under proliferative and differentiative conditions. Expression of Notch receptors and ligands were evaluated by quantitative PCR and Western blot. The effect of Notch inhibition and induction on oil production was also assessed.
Human meibomian gland epithelial cell expressed Notch1, Notch2, Notch3, Jagged1, Jagged2, Delta-like 1, and Delta-like 3. The level of cleaved (activated) Notch1 strongly increased with differentiation. The expression of Notch3 was inversely correlated with proliferation. Induction and inhibition of Notch1 led to an increase and decrease in the amount of oil production, respectively.
Notch signaling appears to play an important role in human meibomian gland epithelial differentiation and oil production. This may provide a potential therapeutic pathway for treating meibomian gland dysfunction.</description><subject>Biochemistry and Molecular Biology</subject><subject>Blotting, Western</subject><subject>Cell Differentiation - genetics</subject><subject>Cells, Cultured</subject><subject>Epithelial Cells - pathology</subject><subject>Eyelid Diseases - genetics</subject><subject>Eyelid Diseases - metabolism</subject><subject>Eyelid Diseases - pathology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Meibomian Glands - pathology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptor, Notch1 - biosynthesis</subject><subject>Receptor, Notch1 - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Signal Transduction</subject><issn>1552-5783</issn><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkD1PwzAQhi0EoqUwsqKMLCk-f8TOggSlFKQCAzBbTuK0Rq5d4rQS_56UQlWmO-kevXf3IHQOeAiQiSsb1nEIPAVJIT9AfeCcpFxIerjX99BJjB8YEwCCj1GPZDmjwGQf3T6Htpwnr3bmtbN-llifPBlbhIXVPpk47atkvLTt3DirXTIyziV3tq5NY3xrdWuDP0VHtXbRnP3WAXq_H7-NHtLpy-RxdDNNS8Zpm_KMVVoYnutMFkyUhdCEC5FXRlaYGs1LJkVGBK4IkFxUueR1UWBCC0KzDFM6QNfb3OWqWJiq7A5otFPLxi5086WCtur_xNu5moW1YkISSUgXcPkb0ITPlYmtWthYdh9pb8IqKhACGBMgZYemW7RsQoyNqXdrAKuNd7XxroCrH-8df7F_247-E02_AZRufu0</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Gidfar, Sanaz</creator><creator>Afsharkhamseh, Neda</creator><creator>Sanjari, Sara</creator><creator>Djalilian, Ali R</creator><general>The Association for Research in Vision and Ophthalmology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160301</creationdate><title>Notch Signaling in Meibomian Gland Epithelial Cell Differentiation</title><author>Gidfar, Sanaz ; Afsharkhamseh, Neda ; Sanjari, Sara ; Djalilian, Ali R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-564da7e59a68b47cb7a25779de8d03ea5c4876270d21297d985fbb023b2366033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biochemistry and Molecular Biology</topic><topic>Blotting, Western</topic><topic>Cell Differentiation - genetics</topic><topic>Cells, Cultured</topic><topic>Epithelial Cells - pathology</topic><topic>Eyelid Diseases - genetics</topic><topic>Eyelid Diseases - metabolism</topic><topic>Eyelid Diseases - pathology</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Meibomian Glands - pathology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptor, Notch1 - biosynthesis</topic><topic>Receptor, Notch1 - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gidfar, Sanaz</creatorcontrib><creatorcontrib>Afsharkhamseh, Neda</creatorcontrib><creatorcontrib>Sanjari, Sara</creatorcontrib><creatorcontrib>Djalilian, Ali R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gidfar, Sanaz</au><au>Afsharkhamseh, Neda</au><au>Sanjari, Sara</au><au>Djalilian, Ali R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Notch Signaling in Meibomian Gland Epithelial Cell Differentiation</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>57</volume><issue>3</issue><spage>859</spage><epage>865</epage><pages>859-865</pages><issn>1552-5783</issn><issn>0146-0404</issn><eissn>1552-5783</eissn><abstract>Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro.
An immortalized human meibomian gland epithelial cell (HMGEC) line was cultured under proliferative and differentiative conditions. Expression of Notch receptors and ligands were evaluated by quantitative PCR and Western blot. The effect of Notch inhibition and induction on oil production was also assessed.
Human meibomian gland epithelial cell expressed Notch1, Notch2, Notch3, Jagged1, Jagged2, Delta-like 1, and Delta-like 3. The level of cleaved (activated) Notch1 strongly increased with differentiation. The expression of Notch3 was inversely correlated with proliferation. Induction and inhibition of Notch1 led to an increase and decrease in the amount of oil production, respectively.
Notch signaling appears to play an important role in human meibomian gland epithelial differentiation and oil production. This may provide a potential therapeutic pathway for treating meibomian gland dysfunction.</abstract><cop>United States</cop><pub>The Association for Research in Vision and Ophthalmology</pub><pmid>26943148</pmid><doi>10.1167/iovs.15-18319</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-5783 |
| ispartof | Investigative ophthalmology & visual science, 2016-03, Vol.57 (3), p.859-865 |
| issn | 1552-5783 0146-0404 1552-5783 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4782822 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biochemistry and Molecular Biology Blotting, Western Cell Differentiation - genetics Cells, Cultured Epithelial Cells - pathology Eyelid Diseases - genetics Eyelid Diseases - metabolism Eyelid Diseases - pathology Gene Expression Regulation Humans Meibomian Glands - pathology Real-Time Polymerase Chain Reaction Receptor, Notch1 - biosynthesis Receptor, Notch1 - genetics RNA, Messenger - genetics Signal Transduction |
| title | Notch Signaling in Meibomian Gland Epithelial Cell Differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T13%3A33%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Notch%20Signaling%20in%20Meibomian%20Gland%20Epithelial%20Cell%20Differentiation&rft.jtitle=Investigative%20ophthalmology%20&%20visual%20science&rft.au=Gidfar,%20Sanaz&rft.date=2016-03-01&rft.volume=57&rft.issue=3&rft.spage=859&rft.epage=865&rft.pages=859-865&rft.issn=1552-5783&rft.eissn=1552-5783&rft_id=info:doi/10.1167/iovs.15-18319&rft_dat=%3Cproquest_pubme%3E1771447188%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771447188&rft_id=info:pmid/26943148&rfr_iscdi=true |