Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms

Imaging of fully hydrated, vitrified biological samples by electron tomography yields structural information about cellular protein complexes in situ. Here we present a computational procedure that removes artifacts of three-dimensional reconstruction caused by contamination present in samples durin...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biophysical journal 2016-02, Vol.110 (4), p.850-859
Hauptverfasser:	Fernandez, Jose-Jesus, Laugks, Ulrike, Schaffer, Miroslava, Bäuerlein, Felix J.B., Khoshouei, Maryam, Baumeister, Wolfgang, Lucic, Vladan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Biophysics Cells Contamination Electron Microscope Tomography - methods Electron microscopes Fiducial Markers HeLa Cells Humans Image Processing, Computer-Assisted Male Neurons - cytology Phantoms, Imaging Proteins Rats Rats, Wistar Tomography Vitrification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!