Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study
The predominant characteristic of malignant glioma is the presence of invading tumor cells in the peritumoral zone. Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements...
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| Veröffentlicht in: | Oncology letters 2016-03, Vol.11 (3), p.2106-2110 |
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| creator | NAKAJIMA, SYOICHI MORII, KEN TAKAHASHI, HITOSHI FUJII, YUKIHIKO YAMANAKA, RYUYA |
| description | The predominant characteristic of malignant glioma is the presence of invading tumor cells in the peritumoral zone. Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas. |
| doi_str_mv | 10.3892/ol.2016.4205 |
| format | Article |
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Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2016.4205</identifier><identifier>PMID: 26998130</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Brain cancer ; Brain research ; Care and treatment ; Cell cycle ; Complications and side effects ; Deoxyribonucleic acid ; DNA ; Glioma ; Gliomas ; high grade glioma ; invading zone ; laser scanning cytometry ; Lasers in medicine ; Medical prognosis ; Nervous system ; Neurosurgery ; NMR ; Nuclear magnetic resonance ; Oncology ; Patients ; prognostic significance ; S phase ; Software ; Standard deviation ; Studies ; Tumors</subject><ispartof>Oncology letters, 2016-03, Vol.11 (3), p.2106-2110</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><rights>Copyright © 2016, Spandidos Publications 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-bd187f0155ed0d9571b8b8ae1701850fb20ab446470cc6104c86f43a67f9f8443</citedby><cites>FETCH-LOGICAL-c539t-bd187f0155ed0d9571b8b8ae1701850fb20ab446470cc6104c86f43a67f9f8443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774597/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774597/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,5572,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26998130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAKAJIMA, SYOICHI</creatorcontrib><creatorcontrib>MORII, KEN</creatorcontrib><creatorcontrib>TAKAHASHI, HITOSHI</creatorcontrib><creatorcontrib>FUJII, YUKIHIKO</creatorcontrib><creatorcontrib>YAMANAKA, RYUYA</creatorcontrib><title>Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>The predominant characteristic of malignant glioma is the presence of invading tumor cells in the peritumoral zone. Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas.</description><subject>Brain cancer</subject><subject>Brain research</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Complications and side effects</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Glioma</subject><subject>Gliomas</subject><subject>high grade glioma</subject><subject>invading zone</subject><subject>laser scanning cytometry</subject><subject>Lasers in medicine</subject><subject>Medical prognosis</subject><subject>Nervous system</subject><subject>Neurosurgery</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Oncology</subject><subject>Patients</subject><subject>prognostic significance</subject><subject>S phase</subject><subject>Software</subject><subject>Standard deviation</subject><subject>Studies</subject><subject>Tumors</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkl2P1CAUhhujcTfr3nltSDTGCztCS4F6YbLZ-JVMsibqNaEUWjYUKtA1sz_I3ykzs447Rs4FH-c5L4fDKYqnCK5q1lZvvF1VEJEVrmDzoDhFtK1KBFn18LCm-KQ4j_Ea5tEQxBh5XJxUpG0ZquFp8etL8IPzMRkJohmc0UYKJxXwGnwt51FEBXQQMhnvIjAOzCqYtEw-CJu3N6I3bgC33ikgnLCbW9WDbgNsjgsgZim39ctN8pNKYbNTEMkolyL4adIIRjOM5RBEr8BgjZ_EW3AB5qCsmYwTOSKmpd88KR5pYaM6v5vPiu8f3n-7_FSurz5-vrxYl7Kp21R2PWJUQ9Q0qod921DUsY4JhShErIG6q6DoMCaYQikJglgyonEtCNWtZhjXZ8W7ve68dJPqZc4zP5TPwUw5F-6F4cceZ0Y--BuOKcVNS7PAqzuB4H8sKiY-mSiVtcIpv0SOWEUIqShGGX3-D3rtl5CLmKm2rkiWY_AvNQiruHHa53vlVpRfYExJzRpcZWr1HypbryYj8-9ok8-PAl7eCxiVsGmM3i67fz4GX-9BGXyMQelDMRDk2x7k3vJtD_JtD2b82f0CHuA_HZeBF3sgzsL1pvfxwFytS5htp_MbOXrkiQ</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>NAKAJIMA, SYOICHI</creator><creator>MORII, KEN</creator><creator>TAKAHASHI, HITOSHI</creator><creator>FUJII, YUKIHIKO</creator><creator>YAMANAKA, RYUYA</creator><general>D.A. 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Distinguishing between tumor cells and normal cells in a peritumoral lesion is challenging. Therefore, the aim of the present study was to investigate the cell-cycle phase measurements of fixed paraffin-embedded specimens from the peritumoral invading zone of high-grade gliomas using laser scanning cytometry. A total of 12 high-grade gliomas (2 anaplastic astrocytomas and 10 glioblastomas) were studied. The tumor core and peritumoral invading zone of each tumor specimen were investigated. Tissue sections (50 µm) from the paraffin blocks were deparaffinized, rehydrated and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide and placed on microscope slides. A slight trend for an increased S-phase fraction in the peritumoral invading zone compared with the tumor core was observed (P=0.24). Additionally, there was a trend for a decrease in the overall survival time of patients with increasing peritumoral invading zone S-phase fraction (P=0.12). These data suggest that laser scanning cytometry is a powerful and clinically relevant tool for the objective analysis of the cell cycle in malignant gliomas.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>26998130</pmid><doi>10.3892/ol.2016.4205</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology letters, 2016-03, Vol.11 (3), p.2106-2110 |
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| language | eng |
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| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Brain cancer Brain research Care and treatment Cell cycle Complications and side effects Deoxyribonucleic acid DNA Glioma Gliomas high grade glioma invading zone laser scanning cytometry Lasers in medicine Medical prognosis Nervous system Neurosurgery NMR Nuclear magnetic resonance Oncology Patients prognostic significance S phase Software Standard deviation Studies Tumors |
| title | Prognostic significance of S-phase fractions in peritumoral invading zone analyzed by laser scanning cytometry in patients with high-grade glioma: A preliminary study |
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