Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma
The present study aimed to determine the expression levels and biological functions of microRNA-202 (miR-202) in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain...
Gespeichert in:
| Veröffentlicht in: | Experimental and therapeutic medicine 2016-03, Vol.11 (3), p.951-956 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 956 |
|---|---|
| container_issue | 3 |
| container_start_page | 951 |
| container_title | Experimental and therapeutic medicine |
| container_volume | 11 |
| creator | MA, GUOLIANG ZHANG, FENGMEI DONG, XUEGUANG WANG, XIAOLI REN, YUGUO |
| description | The present study aimed to determine the expression levels and biological functions of microRNA-202 (miR-202) in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain reaction was performed to measure the expression levels of miR-202. In order to investigate the effects of miR-202 expression levels on the proliferative, migratory and invasive abilities of ESCC cells, methylthiazolyl-tetrazolium bromide proliferation, in vitro scratch and Transwell® chamber assays were performed. Expression levels of miR-202 were significantly decreased in the peripheral blood of patients with ESCC, which is associated with the degree of cell differentiation and lymph node metastasis (P |
| doi_str_mv | 10.3892/etm.2016.3014 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4774355</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A448338656</galeid><sourcerecordid>A448338656</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-910f90179e3f205f90a7d8869b644639732a38ab5c0d4499f64d4767e7118c0e3</originalsourceid><addsrcrecordid>eNqFkttrFDEUhwdRbKl99FUCIvgya26Ty4uwlHqBVUHqc8hmzuymzEy2kxnb_veecdfVimAuJOR85-Sc5FcUzxldCGP5Gxi7BadMLQRl8lFxyrTlJaOsenzYU2vYSXGe8zXFVilmTPW0OOHKWkOZOS2aVbolcLcbIOeYepIa0sUwpK-flyWnnMRMfM4pRD9CTW7juCXjFkgHo8840IwekNNu6zfgW5JvJt-lKZMAbUuCH0LsU-efFU8a32Y4P6xnxbd3l1cXH8rVl_cfL5arMlSSjqVltLEUMwfRcFrh3uvaGGXXSkolrBbcC-PXVaC1lNY2StZSKw2aMRMoiLPi7T7ublp3UAfox8G3bjfEzg_3LvnoHlr6uHWb9N1JraWoKgzw-hBgSDcT5NF1Mc-1-B6wLMcMV0rh1Ii-_Au9TtPQY3mOWcE4pkvF_yjKhRbsN7XxLbjYNwmzC_PVbimlEcKoSiG1-AeFvQb8tNRDE_H8gUO5d8AfzXmA5vgSjLpZQQ4V5GYFuVlByL_48_mO9C-9IPBqD-Sd7-tYp3xkLq8-lRT7z0A_AJVOypo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1931023731</pqid></control><display><type>article</type><title>Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma</title><source>PubMed Central</source><creator>MA, GUOLIANG ; ZHANG, FENGMEI ; DONG, XUEGUANG ; WANG, XIAOLI ; REN, YUGUO</creator><creatorcontrib>MA, GUOLIANG ; ZHANG, FENGMEI ; DONG, XUEGUANG ; WANG, XIAOLI ; REN, YUGUO</creatorcontrib><description>The present study aimed to determine the expression levels and biological functions of microRNA-202 (miR-202) in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain reaction was performed to measure the expression levels of miR-202. In order to investigate the effects of miR-202 expression levels on the proliferative, migratory and invasive abilities of ESCC cells, methylthiazolyl-tetrazolium bromide proliferation, in vitro scratch and Transwell® chamber assays were performed. Expression levels of miR-202 were significantly decreased in the peripheral blood of patients with ESCC, which is associated with the degree of cell differentiation and lymph node metastasis (P<0.05). Following miR-202 transfection, cell proliferation was significantly inhibited (P<0.05). Cell migration and invasion was also significantly inhibited by miR-202 transfection (P<0.05). The results of the present study demonstrated that the expression of miR-202 inhibited the proliferation, migration and invasion of ESCC cells. Furthermore, low expression levels of miR-202 were detected in the peripheral blood of patients with ESCC, which is associated with the development, invasion and metastasis of ESCC.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2016.3014</identifier><identifier>PMID: 26998018</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; Chemotherapy ; Complications and side effects ; Esophageal cancer ; esophageal squamous cell carcinoma ; Gene expression ; Genetic aspects ; Health aspects ; invasion ; Lymphatic system ; Medical prognosis ; Metastasis ; MicroRNA ; microRNA-202 ; MicroRNAs ; Properties ; Risk factors ; Squamous cell carcinoma ; Tumors</subject><ispartof>Experimental and therapeutic medicine, 2016-03, Vol.11 (3), p.951-956</ispartof><rights>Copyright © 2016, Spandidos Publications</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><rights>Copyright © 2016, Spandidos Publications 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-910f90179e3f205f90a7d8869b644639732a38ab5c0d4499f64d4767e7118c0e3</citedby><cites>FETCH-LOGICAL-c540t-910f90179e3f205f90a7d8869b644639732a38ab5c0d4499f64d4767e7118c0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774355/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774355/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26998018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MA, GUOLIANG</creatorcontrib><creatorcontrib>ZHANG, FENGMEI</creatorcontrib><creatorcontrib>DONG, XUEGUANG</creatorcontrib><creatorcontrib>WANG, XIAOLI</creatorcontrib><creatorcontrib>REN, YUGUO</creatorcontrib><title>Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The present study aimed to determine the expression levels and biological functions of microRNA-202 (miR-202) in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain reaction was performed to measure the expression levels of miR-202. In order to investigate the effects of miR-202 expression levels on the proliferative, migratory and invasive abilities of ESCC cells, methylthiazolyl-tetrazolium bromide proliferation, in vitro scratch and Transwell® chamber assays were performed. Expression levels of miR-202 were significantly decreased in the peripheral blood of patients with ESCC, which is associated with the degree of cell differentiation and lymph node metastasis (P<0.05). Following miR-202 transfection, cell proliferation was significantly inhibited (P<0.05). Cell migration and invasion was also significantly inhibited by miR-202 transfection (P<0.05). The results of the present study demonstrated that the expression of miR-202 inhibited the proliferation, migration and invasion of ESCC cells. Furthermore, low expression levels of miR-202 were detected in the peripheral blood of patients with ESCC, which is associated with the development, invasion and metastasis of ESCC.</description><subject>Angiogenesis</subject><subject>Chemotherapy</subject><subject>Complications and side effects</subject><subject>Esophageal cancer</subject><subject>esophageal squamous cell carcinoma</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>invasion</subject><subject>Lymphatic system</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>microRNA-202</subject><subject>MicroRNAs</subject><subject>Properties</subject><subject>Risk factors</subject><subject>Squamous cell carcinoma</subject><subject>Tumors</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkttrFDEUhwdRbKl99FUCIvgya26Ty4uwlHqBVUHqc8hmzuymzEy2kxnb_veecdfVimAuJOR85-Sc5FcUzxldCGP5Gxi7BadMLQRl8lFxyrTlJaOsenzYU2vYSXGe8zXFVilmTPW0OOHKWkOZOS2aVbolcLcbIOeYepIa0sUwpK-flyWnnMRMfM4pRD9CTW7juCXjFkgHo8840IwekNNu6zfgW5JvJt-lKZMAbUuCH0LsU-efFU8a32Y4P6xnxbd3l1cXH8rVl_cfL5arMlSSjqVltLEUMwfRcFrh3uvaGGXXSkolrBbcC-PXVaC1lNY2StZSKw2aMRMoiLPi7T7ublp3UAfox8G3bjfEzg_3LvnoHlr6uHWb9N1JraWoKgzw-hBgSDcT5NF1Mc-1-B6wLMcMV0rh1Ii-_Au9TtPQY3mOWcE4pkvF_yjKhRbsN7XxLbjYNwmzC_PVbimlEcKoSiG1-AeFvQb8tNRDE_H8gUO5d8AfzXmA5vgSjLpZQQ4V5GYFuVlByL_48_mO9C-9IPBqD-Sd7-tYp3xkLq8-lRT7z0A_AJVOypo</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>MA, GUOLIANG</creator><creator>ZHANG, FENGMEI</creator><creator>DONG, XUEGUANG</creator><creator>WANG, XIAOLI</creator><creator>REN, YUGUO</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160301</creationdate><title>Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma</title><author>MA, GUOLIANG ; ZHANG, FENGMEI ; DONG, XUEGUANG ; WANG, XIAOLI ; REN, YUGUO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-910f90179e3f205f90a7d8869b644639732a38ab5c0d4499f64d4767e7118c0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Angiogenesis</topic><topic>Chemotherapy</topic><topic>Complications and side effects</topic><topic>Esophageal cancer</topic><topic>esophageal squamous cell carcinoma</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>invasion</topic><topic>Lymphatic system</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>microRNA-202</topic><topic>MicroRNAs</topic><topic>Properties</topic><topic>Risk factors</topic><topic>Squamous cell carcinoma</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MA, GUOLIANG</creatorcontrib><creatorcontrib>ZHANG, FENGMEI</creatorcontrib><creatorcontrib>DONG, XUEGUANG</creatorcontrib><creatorcontrib>WANG, XIAOLI</creatorcontrib><creatorcontrib>REN, YUGUO</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MA, GUOLIANG</au><au>ZHANG, FENGMEI</au><au>DONG, XUEGUANG</au><au>WANG, XIAOLI</au><au>REN, YUGUO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>951</spage><epage>956</epage><pages>951-956</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The present study aimed to determine the expression levels and biological functions of microRNA-202 (miR-202) in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain reaction was performed to measure the expression levels of miR-202. In order to investigate the effects of miR-202 expression levels on the proliferative, migratory and invasive abilities of ESCC cells, methylthiazolyl-tetrazolium bromide proliferation, in vitro scratch and Transwell® chamber assays were performed. Expression levels of miR-202 were significantly decreased in the peripheral blood of patients with ESCC, which is associated with the degree of cell differentiation and lymph node metastasis (P<0.05). Following miR-202 transfection, cell proliferation was significantly inhibited (P<0.05). Cell migration and invasion was also significantly inhibited by miR-202 transfection (P<0.05). The results of the present study demonstrated that the expression of miR-202 inhibited the proliferation, migration and invasion of ESCC cells. Furthermore, low expression levels of miR-202 were detected in the peripheral blood of patients with ESCC, which is associated with the development, invasion and metastasis of ESCC.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>26998018</pmid><doi>10.3892/etm.2016.3014</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-0981 |
| ispartof | Experimental and therapeutic medicine, 2016-03, Vol.11 (3), p.951-956 |
| issn | 1792-0981 1792-1015 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4774355 |
| source | PubMed Central |
| subjects | Angiogenesis Chemotherapy Complications and side effects Esophageal cancer esophageal squamous cell carcinoma Gene expression Genetic aspects Health aspects invasion Lymphatic system Medical prognosis Metastasis MicroRNA microRNA-202 MicroRNAs Properties Risk factors Squamous cell carcinoma Tumors |
| title | Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T01%3A01%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low%20expression%20of%20microRNA-202%20is%20associated%20with%20the%20metastasis%20of%20esophageal%20squamous%20cell%20carcinoma&rft.jtitle=Experimental%20and%20therapeutic%20medicine&rft.au=MA,%20GUOLIANG&rft.date=2016-03-01&rft.volume=11&rft.issue=3&rft.spage=951&rft.epage=956&rft.pages=951-956&rft.issn=1792-0981&rft.eissn=1792-1015&rft_id=info:doi/10.3892/etm.2016.3014&rft_dat=%3Cgale_pubme%3EA448338656%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1931023731&rft_id=info:pmid/26998018&rft_galeid=A448338656&rfr_iscdi=true |