Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria
The diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulne...
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| Veröffentlicht in: | Journal of negative results in biomedicine 2016-03, Vol.15 (3), p.3-3, Article 3 |
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| creator | Herrera, Mariana Aguilar, Yudy Alexandra Rueda, Zulma Vanessa Muskus, Carlos Vélez, Lázaro Agustín |
| description | The diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulness of these techniques. We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specific PCR commercial kits, paired serology, and urinary antigen.
A total of 225 persons were included. Of these, 10 patients showed serological evidence of L. pneumophila infection, 30 of M. pneumoniae, and 18 of C. pneumoniae; 20 individuals showed no CAP. The sensitivities were mPCR-NPS = 23.1%, mPCR-IS = 57.1%, Seeplex®-IS = 52.4%, and Speed-oligo®-NPA/NPS = 11.1%, and the specificities were mPCR-NPS = 97.1%, mPCR-IS = 77.8%, Seeplex®-IS = 92.6%, and Speed-oligo®-NPA/NPS = 96.1%. The concordance between tests was poor (kappa |
| doi_str_mv | 10.1186/s12952-016-0047-y |
| format | Article |
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A total of 225 persons were included. Of these, 10 patients showed serological evidence of L. pneumophila infection, 30 of M. pneumoniae, and 18 of C. pneumoniae; 20 individuals showed no CAP. The sensitivities were mPCR-NPS = 23.1%, mPCR-IS = 57.1%, Seeplex®-IS = 52.4%, and Speed-oligo®-NPA/NPS = 11.1%, and the specificities were mPCR-NPS = 97.1%, mPCR-IS = 77.8%, Seeplex®-IS = 92.6%, and Speed-oligo®-NPA/NPS = 96.1%. The concordance between tests was poor (kappa <0.4), except for the concordance between mPCR and the commercial kit in IS (0.67). In individuals with no evidence of CAP, positive reactions were observed in paired serology and in all PCRs.
All PCRs had good specificity but low sensitivity in nasopharyngeal samples. The sensitivity of mPCR and Seeplex® in IS was approximately 60%; thus, better diagnostic techniques for these three bacteria are required.</description><identifier>ISSN: 1477-5751</identifier><identifier>EISSN: 1477-5751</identifier><identifier>DOI: 10.1186/s12952-016-0047-y</identifier><identifier>PMID: 26932735</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Aged ; Bacterial infections ; Care and treatment ; Chlamydophila pneumoniae - isolation & purification ; Community-Acquired Infections - diagnosis ; Community-Acquired Infections - microbiology ; Complications and side effects ; Diagnosis ; Female ; Genetic aspects ; Humans ; Legionella pneumophila ; Legionella pneumophila - isolation & purification ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Mycoplasma pneumoniae - isolation & purification ; Pneumonia, Bacterial - diagnosis ; Pneumonia, Bacterial - microbiology ; Polymerase Chain Reaction - methods ; Prevention</subject><ispartof>Journal of negative results in biomedicine, 2016-03, Vol.15 (3), p.3-3, Article 3</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>Herrera et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-ebffa47604eadfacbdc15133ed2b3dd0480a8950b805f9ff700eac30fdd48e493</citedby><cites>FETCH-LOGICAL-c494t-ebffa47604eadfacbdc15133ed2b3dd0480a8950b805f9ff700eac30fdd48e493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774004/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774004/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26932735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herrera, Mariana</creatorcontrib><creatorcontrib>Aguilar, Yudy Alexandra</creatorcontrib><creatorcontrib>Rueda, Zulma Vanessa</creatorcontrib><creatorcontrib>Muskus, Carlos</creatorcontrib><creatorcontrib>Vélez, Lázaro Agustín</creatorcontrib><title>Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria</title><title>Journal of negative results in biomedicine</title><addtitle>J Negat Results Biomed</addtitle><description>The diagnosis of community-acquired pneumonia (CAP) caused by Legionella pneumophila, Mycoplasma pneumoniae, and Chlamydophila pneumoniae is traditionally based on cultures and serology, which have special requirements, are time-consuming, and offer delayed results that limit their clinical usefulness of these techniques. We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specific PCR commercial kits, paired serology, and urinary antigen.
A total of 225 persons were included. Of these, 10 patients showed serological evidence of L. pneumophila infection, 30 of M. pneumoniae, and 18 of C. pneumoniae; 20 individuals showed no CAP. The sensitivities were mPCR-NPS = 23.1%, mPCR-IS = 57.1%, Seeplex®-IS = 52.4%, and Speed-oligo®-NPA/NPS = 11.1%, and the specificities were mPCR-NPS = 97.1%, mPCR-IS = 77.8%, Seeplex®-IS = 92.6%, and Speed-oligo®-NPA/NPS = 96.1%. The concordance between tests was poor (kappa <0.4), except for the concordance between mPCR and the commercial kit in IS (0.67). In individuals with no evidence of CAP, positive reactions were observed in paired serology and in all PCRs.
All PCRs had good specificity but low sensitivity in nasopharyngeal samples. The sensitivity of mPCR and Seeplex® in IS was approximately 60%; thus, better diagnostic techniques for these three bacteria are required.</description><subject>Adult</subject><subject>Aged</subject><subject>Bacterial infections</subject><subject>Care and treatment</subject><subject>Chlamydophila pneumoniae - isolation & purification</subject><subject>Community-Acquired Infections - diagnosis</subject><subject>Community-Acquired Infections - microbiology</subject><subject>Complications and side effects</subject><subject>Diagnosis</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Legionella pneumophila</subject><subject>Legionella pneumophila - isolation & purification</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Multiplex Polymerase Chain Reaction</subject><subject>Mycoplasma pneumoniae - isolation & purification</subject><subject>Pneumonia, Bacterial - diagnosis</subject><subject>Pneumonia, Bacterial - microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prevention</subject><issn>1477-5751</issn><issn>1477-5751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptktGK1TAQhoso7rr6AN5IwRtvuk7atGlvhOXgrsKCInodpsnknCxtcjZplb6Az23qWY-7IgkkJN__DzP8WfaSwTljbfM2srKrywJYUwBwUSyPslPGhShqUbPH9-4n2bMYbwBKEI14mp2UTVeVoqpPs58bP-4x2Ohd7k0eKfjBb63CIR9p2nkd8x922uWfN1-KHiPp47PxIZ92lGuLW-ejjate-XGcnZ2WAtXtbEPi947m0TuLucJ5NeiXHKdl_7tGj2qiYPF59sTgEOnF3XmWfbt8_3Xzobj-dPVxc3FdKN7xqaDeGOSiAU6oDapeK1azqiJd9pXWwFvAtquhb6E2nTECgFBVYLTmLfGuOsveHXz3cz-SVuSmgIPcBztiWKRHKx_-OLuTW_9dpknyNONk8ObOIPjbmeIkRxsVDQM68nOUTAgoSyhFndDX_6A3fg4utZeoTrRt3QH7S21xIGmd8amuWk3lBee8TbsRiTr_D5WWptEq78jY9P5AwA4CFXyMgcyxRwZyDY88hEem8Mg1PHJJmlf3h3NU_ElL9QuYJMMQ</recordid><startdate>20160302</startdate><enddate>20160302</enddate><creator>Herrera, Mariana</creator><creator>Aguilar, Yudy Alexandra</creator><creator>Rueda, Zulma Vanessa</creator><creator>Muskus, Carlos</creator><creator>Vélez, Lázaro Agustín</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160302</creationdate><title>Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria</title><author>Herrera, Mariana ; 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We sought to develop a multiplex PCR (mPCR) method to diagnosis these bacterial infections in CAP patients and to compare the diagnostic yields obtained from mPCR of nasopharyngeal aspirates (NPAs), nasopharyngeal swabs (NPSs), and induced sputum (IS) with those obtained with specific PCR commercial kits, paired serology, and urinary antigen.
A total of 225 persons were included. Of these, 10 patients showed serological evidence of L. pneumophila infection, 30 of M. pneumoniae, and 18 of C. pneumoniae; 20 individuals showed no CAP. The sensitivities were mPCR-NPS = 23.1%, mPCR-IS = 57.1%, Seeplex®-IS = 52.4%, and Speed-oligo®-NPA/NPS = 11.1%, and the specificities were mPCR-NPS = 97.1%, mPCR-IS = 77.8%, Seeplex®-IS = 92.6%, and Speed-oligo®-NPA/NPS = 96.1%. The concordance between tests was poor (kappa <0.4), except for the concordance between mPCR and the commercial kit in IS (0.67). In individuals with no evidence of CAP, positive reactions were observed in paired serology and in all PCRs.
All PCRs had good specificity but low sensitivity in nasopharyngeal samples. The sensitivity of mPCR and Seeplex® in IS was approximately 60%; thus, better diagnostic techniques for these three bacteria are required.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26932735</pmid><doi>10.1186/s12952-016-0047-y</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Bacterial infections Care and treatment Chlamydophila pneumoniae - isolation & purification Community-Acquired Infections - diagnosis Community-Acquired Infections - microbiology Complications and side effects Diagnosis Female Genetic aspects Humans Legionella pneumophila Legionella pneumophila - isolation & purification Male Middle Aged Multiplex Polymerase Chain Reaction Mycoplasma pneumoniae - isolation & purification Pneumonia, Bacterial - diagnosis Pneumonia, Bacterial - microbiology Polymerase Chain Reaction - methods Prevention |
| title | Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria |
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