A New Automated Method and Sample Data Flow for Analysis of Volatile Nitrosamines in Human Urine

A New Automated Method and Sample Data Flow for Analysis of Volatile Nitrosamines in Human Urine

Volatile nitrosamines (VNAs) are a group of compounds classified as probable (group 2A) and possible (group 2B) carcinogens in humans. Along with certain foods and contaminated drinking water, VNAs are detected at high levels in tobacco products and in both mainstream and sidestream smoke. Our labor...

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Veröffentlicht in:American journal of analytical chemistry 2016-02, Vol.7 (2), p.165-178
Hauptverfasser: Hodgson, James A, Seyler, Tiffany H, McGahee, Ernest, Arnstein, Stephen, Wang, Lanqing
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container_issue 2
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container_title American journal of analytical chemistry
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creator Hodgson, James A
Seyler, Tiffany H
McGahee, Ernest
Arnstein, Stephen
Wang, Lanqing
description Volatile nitrosamines (VNAs) are a group of compounds classified as probable (group 2A) and possible (group 2B) carcinogens in humans. Along with certain foods and contaminated drinking water, VNAs are detected at high levels in tobacco products and in both mainstream and sidestream smoke. Our laboratory monitors six urinary VNAs-N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosopiperidine (NPIP), N-nitrosopyrrolidine (NPYR), and N-nitrosomorpholine (NMOR)-using isotope dilution GC-MS/MS (QQQ) for large population studies such as the National Health and Nutrition Examination Survey (NHANES). In this paper, we report for the first time a new automated sample preparation method to more efficiently quantitate these VNAs. Automation is done using Hamilton STAR and Caliper Staccato workstations. This new automated method reduces sample preparation time from 4 hours to 2.5 hours while maintaining precision (inter-run CV < 10%) and accuracy (85% - 111%). More importantly this method increases sample throughput while maintaining a low limit of detection (
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title A New Automated Method and Sample Data Flow for Analysis of Volatile Nitrosamines in Human Urine
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