Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival
Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithel...
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| Veröffentlicht in: | Oncotarget 2015-11, Vol.6 (36), p.38681-38694 |
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| creator | Punt, Simone Corver, Willem E van der Zeeuw, Sander A J Kielbasa, Szymon M Osse, Elisabeth M Buermans, Henk P J de Kroon, Cornelis D Jordanova, Ekaterina S Gorter, Arko |
| description | Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithelial cells and CD45+ tumor-infiltrating immune cells obtained from squamous cell cervical cancer samples (n = 24). Total RNA was sequenced and differential gene expression analysis of the CD45+ immune cell fractions identified TCL1A as a novel marker for predicting improved survival (p = 0.007). This finding was validated using qRT-PCR (p = 0.005) and partially validated using immunohistochemistry (p = 0.083). Importantly, TCL1A was found to be expressed in a subpopulation of B cells (CD3-/CD19+/CD10+/CD34-) using multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined in total tumor samples from a separate patient cohort using qRT-PCR (n = 52), was also correlated with improved survival (p = 0.027). This is the first study demonstrating the prognostic value of separating tumor epithelial cells from tumor-infiltrating immune cells and determining their RNA expression profile for identifying putative cancer biomarkers. Our results suggest that intratumoral TCL1A+ B cells are important for controlling cervical cancer development. |
| doi_str_mv | 10.18632/oncotarget.4526 |
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Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithelial cells and CD45+ tumor-infiltrating immune cells obtained from squamous cell cervical cancer samples (n = 24). Total RNA was sequenced and differential gene expression analysis of the CD45+ immune cell fractions identified TCL1A as a novel marker for predicting improved survival (p = 0.007). This finding was validated using qRT-PCR (p = 0.005) and partially validated using immunohistochemistry (p = 0.083). Importantly, TCL1A was found to be expressed in a subpopulation of B cells (CD3-/CD19+/CD10+/CD34-) using multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined in total tumor samples from a separate patient cohort using qRT-PCR (n = 52), was also correlated with improved survival (p = 0.027). This is the first study demonstrating the prognostic value of separating tumor epithelial cells from tumor-infiltrating immune cells and determining their RNA expression profile for identifying putative cancer biomarkers. Our results suggest that intratumoral TCL1A+ B cells are important for controlling cervical cancer development.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.4526</identifier><identifier>PMID: 26299617</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Adult ; Aged ; Aged, 80 and over ; B-Lymphocytes - metabolism ; Female ; Gene Expression Profiling ; Humans ; Middle Aged ; Prognosis ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Research Paper ; Survival Analysis ; Tumor Microenvironment ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - mortality ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Oncotarget, 2015-11, Vol.6 (36), p.38681-38694</ispartof><rights>Copyright: © 2015 Punt et al. 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-1c18f7a8895c2dbe4d67f90a44549f11ec91211ceb5e9ef0b164fa5532287d853</citedby><cites>FETCH-LOGICAL-c396t-1c18f7a8895c2dbe4d67f90a44549f11ec91211ceb5e9ef0b164fa5532287d853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770729/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770729/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26299617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Punt, Simone</creatorcontrib><creatorcontrib>Corver, Willem E</creatorcontrib><creatorcontrib>van der Zeeuw, Sander A J</creatorcontrib><creatorcontrib>Kielbasa, Szymon M</creatorcontrib><creatorcontrib>Osse, Elisabeth M</creatorcontrib><creatorcontrib>Buermans, Henk P J</creatorcontrib><creatorcontrib>de Kroon, Cornelis D</creatorcontrib><creatorcontrib>Jordanova, Ekaterina S</creatorcontrib><creatorcontrib>Gorter, Arko</creatorcontrib><title>Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithelial cells and CD45+ tumor-infiltrating immune cells obtained from squamous cell cervical cancer samples (n = 24). Total RNA was sequenced and differential gene expression analysis of the CD45+ immune cell fractions identified TCL1A as a novel marker for predicting improved survival (p = 0.007). This finding was validated using qRT-PCR (p = 0.005) and partially validated using immunohistochemistry (p = 0.083). Importantly, TCL1A was found to be expressed in a subpopulation of B cells (CD3-/CD19+/CD10+/CD34-) using multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined in total tumor samples from a separate patient cohort using qRT-PCR (n = 52), was also correlated with improved survival (p = 0.027). This is the first study demonstrating the prognostic value of separating tumor epithelial cells from tumor-infiltrating immune cells and determining their RNA expression profile for identifying putative cancer biomarkers. Our results suggest that intratumoral TCL1A+ B cells are important for controlling cervical cancer development.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>B-Lymphocytes - metabolism</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Prognosis</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Research Paper</subject><subject>Survival Analysis</subject><subject>Tumor Microenvironment</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - mortality</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9vFSEUxYnR2KZ278qwdDN1LsMMsDGpL_5LXuKmxiXhMZc-DDOMwJva7-CHlmdrrWy4hHN-l8sh5CW0FyCHjr2Js43FpGssF7xnwxNyCoqrhvV99_RRfULOc_7e1tVzIZl6Tk7YwJQaQJySX9_2MWBTkpmzTX4pcUJqZhNus880OupCvGlyTAVHajGt3ppArZlrTbOZloCZJlzRhEzL3hT6rspCoPhzSZhzdV1ttnBJK83GlDCYI-nGlz3105LiWk_5ULmrCS_IM1c5eH6_n5GvH95fbT412y8fP28ut43t1FAasCCdMFKq3rJxh3wchFOt4bznygGgVcAALO56VOjaHQzcmfoTjEkxyr47I2_vuMthN-Foca7zB70kP5l0q6Px-v-b2e_1dVw1F6IVTFXA63tAij8OmIuefD6ObWaMh6xBdFKCaoFXaXsntSnmnNA9tIFW_8lR_8tRH3OsllePn_dg-Jta9xssWqA4</recordid><startdate>20151117</startdate><enddate>20151117</enddate><creator>Punt, Simone</creator><creator>Corver, Willem E</creator><creator>van der Zeeuw, Sander A J</creator><creator>Kielbasa, Szymon M</creator><creator>Osse, Elisabeth M</creator><creator>Buermans, Henk P J</creator><creator>de Kroon, Cornelis D</creator><creator>Jordanova, Ekaterina S</creator><creator>Gorter, Arko</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151117</creationdate><title>Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival</title><author>Punt, Simone ; Corver, Willem E ; van der Zeeuw, Sander A J ; Kielbasa, Szymon M ; Osse, Elisabeth M ; Buermans, Henk P J ; de Kroon, Cornelis D ; Jordanova, Ekaterina S ; Gorter, Arko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-1c18f7a8895c2dbe4d67f90a44549f11ec91211ceb5e9ef0b164fa5532287d853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>B-Lymphocytes - metabolism</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Prognosis</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Research Paper</topic><topic>Survival Analysis</topic><topic>Tumor Microenvironment</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - mortality</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Punt, Simone</creatorcontrib><creatorcontrib>Corver, Willem E</creatorcontrib><creatorcontrib>van der Zeeuw, Sander A J</creatorcontrib><creatorcontrib>Kielbasa, Szymon M</creatorcontrib><creatorcontrib>Osse, Elisabeth M</creatorcontrib><creatorcontrib>Buermans, Henk P J</creatorcontrib><creatorcontrib>de Kroon, Cornelis D</creatorcontrib><creatorcontrib>Jordanova, Ekaterina S</creatorcontrib><creatorcontrib>Gorter, Arko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Punt, Simone</au><au>Corver, Willem E</au><au>van der Zeeuw, Sander A J</au><au>Kielbasa, Szymon M</au><au>Osse, Elisabeth M</au><au>Buermans, Henk P J</au><au>de Kroon, Cornelis D</au><au>Jordanova, Ekaterina S</au><au>Gorter, Arko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2015-11-17</date><risdate>2015</risdate><volume>6</volume><issue>36</issue><spage>38681</spage><epage>38694</epage><pages>38681-38694</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithelial cells and CD45+ tumor-infiltrating immune cells obtained from squamous cell cervical cancer samples (n = 24). Total RNA was sequenced and differential gene expression analysis of the CD45+ immune cell fractions identified TCL1A as a novel marker for predicting improved survival (p = 0.007). This finding was validated using qRT-PCR (p = 0.005) and partially validated using immunohistochemistry (p = 0.083). Importantly, TCL1A was found to be expressed in a subpopulation of B cells (CD3-/CD19+/CD10+/CD34-) using multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined in total tumor samples from a separate patient cohort using qRT-PCR (n = 52), was also correlated with improved survival (p = 0.027). 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| source | Freely Accessible Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Adult Aged Aged, 80 and over B-Lymphocytes - metabolism Female Gene Expression Profiling Humans Middle Aged Prognosis Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Research Paper Survival Analysis Tumor Microenvironment Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - mortality Uterine Cervical Neoplasms - pathology |
| title | Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival |
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