19F NMR and DFT Analysis Reveal Structural and Electronic Transition State Features for RhoA-Catalyzed GTP Hydrolysis

Molecular details for RhoA/GAP catalysis of the hydrolysis of GTP to GDP are poorly understood. We use 19F NMR chemical shifts in the MgF3− transition state analogue (TSA) complex as a spectroscopic reporter to indicate electron distribution for the γ‐PO3− oxygens in the corresponding TS, implying t...

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Veröffentlicht in:	Angewandte Chemie International Edition 2016-03, Vol.55 (10), p.3318-3322
Hauptverfasser:	Jin, Yi, Molt Jr, Robert W., Waltho, Jonathan P., Richards, Nigel G. J., Blackburn, G. Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	19F NMR Communication Communications enzyme catalysis GTP hydrolases phosphoryl transfer reaction mechanisms
Online-Zugang:	Volltext
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