A combined binary interaction and phenotypic map of C. elegans cell polarity proteins
The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underli...
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| Veröffentlicht in: | Nature cell biology 2016-03, Vol.18 (3), p.337-346 |
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| creator | Koorman, Thijs Klompstra, Diana van der Voet, Monique Lemmens, Irma Ramalho, João J. Nieuwenhuize, Susan van den Heuvel, Sander Tavernier, Jan Nance, Jeremy Boxem, Mike |
| description | The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode
Caenorhabditis elegans
. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the
C. elegans
embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.
Boxem and colleagues perform a yeast two-hybrid screen to identify interactions between
C. elegans
polarity genes, followed by an RNAi screen to identify the functions of interaction pairs in the establishment and maintenance of cell polarity in various tissues. |
| doi_str_mv | 10.1038/ncb3300 |
| format | Article |
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Caenorhabditis elegans
. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the
C. elegans
embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.
Boxem and colleagues perform a yeast two-hybrid screen to identify interactions between
C. elegans
polarity genes, followed by an RNAi screen to identify the functions of interaction pairs in the establishment and maintenance of cell polarity in various tissues.</description><identifier>ISSN: 1465-7392</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb3300</identifier><identifier>PMID: 26780296</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/106 ; 13/89 ; 14/35 ; 14/63 ; 49/111 ; 49/47 ; 631/136/334/1582/712 ; 631/337/505 ; 631/80/85 ; 64/11 ; Animals ; Caenorhabditis elegans ; Caenorhabditis elegans - cytology ; Caenorhabditis elegans - embryology ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - metabolism ; Cancer Research ; Cell Biology ; Cell Polarity - physiology ; Developmental Biology ; Embryo, Nonmammalian - metabolism ; Genetic aspects ; GTPase-Activating Proteins - genetics ; GTPase-Activating Proteins - metabolism ; Identification and classification ; Life Sciences ; Observations ; Phenotype ; Protein-protein interactions ; resource ; RNA Interference - physiology ; Stem Cells ; Yeasts</subject><ispartof>Nature cell biology, 2016-03, Vol.18 (3), p.337-346</ispartof><rights>Springer Nature Limited 2016</rights><rights>COPYRIGHT 2016 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-2a550d158a3ad60eb65b13d2dc963929b7157ab909fe0fe35f496592a50e30a3</citedby><cites>FETCH-LOGICAL-c601t-2a550d158a3ad60eb65b13d2dc963929b7157ab909fe0fe35f496592a50e30a3</cites><orcidid>0000-0003-3966-4173</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ncb3300$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ncb3300$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26780296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koorman, Thijs</creatorcontrib><creatorcontrib>Klompstra, Diana</creatorcontrib><creatorcontrib>van der Voet, Monique</creatorcontrib><creatorcontrib>Lemmens, Irma</creatorcontrib><creatorcontrib>Ramalho, João J.</creatorcontrib><creatorcontrib>Nieuwenhuize, Susan</creatorcontrib><creatorcontrib>van den Heuvel, Sander</creatorcontrib><creatorcontrib>Tavernier, Jan</creatorcontrib><creatorcontrib>Nance, Jeremy</creatorcontrib><creatorcontrib>Boxem, Mike</creatorcontrib><title>A combined binary interaction and phenotypic map of C. elegans cell polarity proteins</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode
Caenorhabditis elegans
. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the
C. elegans
embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.
Boxem and colleagues perform a yeast two-hybrid screen to identify interactions between
C. elegans
polarity genes, followed by an RNAi screen to identify the functions of interaction pairs in the establishment and maintenance of cell polarity in various tissues.</description><subject>13/1</subject><subject>13/106</subject><subject>13/89</subject><subject>14/35</subject><subject>14/63</subject><subject>49/111</subject><subject>49/47</subject><subject>631/136/334/1582/712</subject><subject>631/337/505</subject><subject>631/80/85</subject><subject>64/11</subject><subject>Animals</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - cytology</subject><subject>Caenorhabditis elegans - embryology</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Cell Polarity - physiology</subject><subject>Developmental Biology</subject><subject>Embryo, Nonmammalian - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koorman, Thijs</au><au>Klompstra, Diana</au><au>van der Voet, Monique</au><au>Lemmens, Irma</au><au>Ramalho, João J.</au><au>Nieuwenhuize, Susan</au><au>van den Heuvel, Sander</au><au>Tavernier, Jan</au><au>Nance, Jeremy</au><au>Boxem, Mike</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A combined binary interaction and phenotypic map of C. elegans cell polarity proteins</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>18</volume><issue>3</issue><spage>337</spage><epage>346</epage><pages>337-346</pages><issn>1465-7392</issn><eissn>1476-4679</eissn><abstract>The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode
Caenorhabditis elegans
. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the
C. elegans
embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.
Boxem and colleagues perform a yeast two-hybrid screen to identify interactions between
C. elegans
polarity genes, followed by an RNAi screen to identify the functions of interaction pairs in the establishment and maintenance of cell polarity in various tissues.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26780296</pmid><doi>10.1038/ncb3300</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3966-4173</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Nature cell biology, 2016-03, Vol.18 (3), p.337-346 |
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| subjects | 13/1 13/106 13/89 14/35 14/63 49/111 49/47 631/136/334/1582/712 631/337/505 631/80/85 64/11 Animals Caenorhabditis elegans Caenorhabditis elegans - cytology Caenorhabditis elegans - embryology Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Cancer Research Cell Biology Cell Polarity - physiology Developmental Biology Embryo, Nonmammalian - metabolism Genetic aspects GTPase-Activating Proteins - genetics GTPase-Activating Proteins - metabolism Identification and classification Life Sciences Observations Phenotype Protein-protein interactions resource RNA Interference - physiology Stem Cells Yeasts |
| title | A combined binary interaction and phenotypic map of C. elegans cell polarity proteins |
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